Curtis Hughey, PhD

Assistant Professor, Division of Molecular Medicine

Curtis Hughey

Contact Info

chughey@umn.edu

Mailing Address:
420 Delaware Street SE
MMC 605 Mayo
8605A (Campus Delivery Code)
Minneapolis, MN 55455

Lab Address:
Molecular and Cellular Biology Building
420 Washington Avenue SE
Rm 5-148
Minneapolis, MN, 55455

Administrative Email
chughey@umn.edu

Assistant Professor, Division of Molecular Medicine


Fellowship, Vanderbilt University, Nashville, TN

PhD, University of Calgary, Calgary, AB

Summary

Dr. Curtis Hughey, PhD, completed his doctoral studies at the University of Calgary in Biochemistry and Molecular Biology. During his doctoral training he received a Canadian Institutes of Health Research Doctoral Scholarship and the Izaak Walton Killam Memorial Scholarship to study the use of stem cell therapy to promote in vivo insulin sensitivity in mouse models of diet-induced obesity and heart failure. With an interest in the genetic and environment regulation of in vivo glucose control, Dr. Hughey then pursued a postdoctoral fellowship at Vanderbilt University. While at Vanderbilt, he received a Canadian Diabetes Association Postdoctoral Fellowship to test the role of liver energy state in the regulation of glucose production during exercise. This work employed a metabolic flux analysis technique that uses stable isotopes, mass spectrometry, and computational modeling to quantify in vivo metabolic fluxes. An emerging research focus for the Hughey Laboratory at the University of Minnesota is to define the role of transmethylation in the metabolic reprogramming that occurs in non-alcoholic fatty liver disease and hepatocellular carcinoma.

Expertise

  • In vivo glucose homeostasis
  • Hepatic intermediary metabolism
  • Non-alcoholic fatty liver disease and the progression to hepatocellular carcinoma

Awards & Recognition

  • Daryl K. Granner Scholar in Diabetes Award, Postdoctoral Fellow, PhD (2018)
  • Diabetes Canada Postdoctoral Fellowship (2014-2017)
  • Izaak Walton Killam Memorial Scholarship (2012-2014)
  • Canadian Institutes of Health Research Canada Graduate Scholarships Doctoral Award (2011-2014)

Research

Research Summary/Interests

Metabolic dysregulation underlies many public health challenges of modern society. The overarching objective of research in the Hughey Laboratory is to identify pathways within complex metabolic networks that can be used for early identification of disease or targeted to prevent or treat metabolic diseases. To accomplish this aim, studies combine the use of stable isotopes, mass spectrometry, and computational modeling to quantify in vivo metabolic fluxes in genetically-engineered mice. Many studies also employ provocative stimuli such as exercise and diet to challenge metabolic networks. Areas of focus are:

  1. The role of dysregulated hepatic transmethylation in the metabolic programming that promotes non-alcoholic fatty liver disease and hepatocellular carcinoma.
  2. Regulation of hepatic glucose formation during acute exercise and in response to habitual exercise.

Publications

  • Hughey C.C., James F.D., Wang Z., Goelzer M., Wasserman D.H. Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation. Molecular Metabolism. 2019 Mar; In press.
  • Hunter R., Hughey C.C., Lantier L., Sundelin E., Peggie M., Zeqiraj E., Sicheri F., Jessen N., Wasserman D.H., Sakamoto K. Metformin reduces liver glucose production by inhibition of fructose-1-6-biphosphatase. Nature Medicine. 2018 Sep;24(9):1395-1406. PMID: 30150719.
  • Hughey C.C., Trefts E., Bracy D.P., James, F.D., Donahue E.P., Wasserman D.H. Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates. Journal of Biological Chemistry. 2018 Jul;293(30):11944-11954. PMID: 29891549.
  • Hughey C.C., James F.D., Bracy D.P., Donahue E.P., Young J.D., Viollet B., Foretz M., Wasserman D.H. Loss of hepatic AMP-activated protein kinase impedes the rate of glycogenolysis but not gluconeogenic fluxes in exercising mice. Journal of Biological Chemistry. 2017 Dec;292(49):20125-20140. PMID: 29038293.
  • Hughey C.C., Wasserman D.H., Lee-Young R.S., Lantier L. Approach to assessing determinants of glucose homeostasis in the conscious mouse. Mammalian Genome. 2014 Oct;25(9-10):522-38. PMID: 25074441.

Teaching

Academic Interests and Focus

Metabolism in Health and Disease