In response to the COVID-19 pandemic, the University Imaging Centers are continuing to provide status updates, and have implemented mandatory cleaning and safety protocols for all users using UIC resources. Please visit our sunrise safety protocols page to learn more 

Electron Microscopes

EM & LM Sample Preparation

Along with SBFSEM, the UIC can provide sample processing for electron and light microscopy samples.

Veterinary Diagnostic Lab (VDL)


Jeol 1440 Plus

The JEM-1400Plus features high resolution/high contrast imaging, outstanding S/TEM analytical performance, 3D tomography, and montaging. This compact, easy-to-use TEM is suitable for biological, polymer, and materials science applications.

Snyder Hall

Hitachi S3500N Variable Pressure SEM


Location: 37D Snyder Hall

The Hitachi S3500N Variable Pressure Scanning Electron Microscope (SEM). Quartz PCI digital imaging, Stage with motor driven X & Y axes, Environmental Secondary Electron Detector (ESED), Absorbed Electron EBIC detector, Robinson Backscattered Electron Detector (BSE), standard Secondary Electron Detector (SED), Infrared ChamberScope, Windows NT Operating System, Emitech K-1150 Cryogenic System, with cryo-prep unit, airlock interface to SEM, sputter coater.

Serial Block-Face SEM

The University Imaging Centers in collaboration with the Mayo Clinic Microscopy and Cell Analysis Core(link is external), and the UMN's Research Computing(link is external) are excited to offer Minnesota researchers access to serial block face electron microscopy and data sharing, thanks to funding from the Minnesota Partnership for Biotechnology and Medical Genomics(link is external).


Recent advances in microscopic imaging are bringing game-changing progress toward understanding public health problems such as aging, neurodegenerative disease, cancer, diabetes, kidney and liver disease and developmental disorders. Scientists can examine molecular features and cell- and tissue-specific relationships at high resolution in three-dimensional (3-D) and large overall volumes. This information can be digitally sampled, offering an unparalleled view of macromolecular complexes, cells and tissues of interest.

The award provides electron microscopy instrumentation, cyberinfrastructure and expertise to researchers at Mayo Clinic, the University of Minnesota and the Hormel Institute. This unique microscopy allows investigators to visualize biological structures at high resolution in 3-D space, bridging the gap between super-resolution and multiphoton optical microscopy and standard transmission electron microscopy.

UIC SBFSEM Sample Preparation

The UIC can prepare the samples and get them into the imaging queue at the Mayo Clinic Microscopy and Cell Analysis Core. We consult with each project, but the general workflow and protocols are available in PDF format below.  We provide all reagents and expertise to help towards a successful outcome.

Mayo Clinic Microscopy and Cell Analysis Core

You can discuss your project with:

Development Tech Coordinator
Microscopy and Cell Analysis Core (MCAC)
RM 1426 Guggenheim
Phone: 507-284-3148
  1. Overview of Preparing and Sending SBFSEM samples to Mayo Clinic
  2. General Overview of SBFSEM Sample Preparation