As part of our ongoing commitment to the University of Minnesota research community and external investigators, the University Imaging Centers hosts technical workshops for hands-on training in imaging.
While we are constantly evolving and expanding our curriculum, past workshops have included: Core Microscopy Concepts, Sample Preparation, Microscopy Instrumentation & Software, Quantitative Analysis, and 3D Reconstruction.
Registration is Full
Both the October and November Confocal Workshops are now at capacity.
If you are interested in being added to a waitlist for potential openings or future opportunities please send us a message at firstname.lastname@example.org. Thank you!
Confocal Workshop - November 2-4, 2021.
The UMN University Imaging Centers (UIC) will be holding a three-day hands on workshop on confocal microscopy November 2-4 at our Jackson Hall facility on the University of Minnesota-Twin Cities East Bank campus.
|Registration Level||University of Minnesota (Internal)||External Investigators|
The goal of the workshop is to provide a clear conceptual framework for the operation of multiple modalities of confocal microscopes and instruct the participants on the practical aspects of image acquisition on multiple systems. The workshop will consist of lectures from UIC staff and invited instructors and over 15 hours of microscope time. Participants will receive training in laser scanning and spinning disk confocal microscopes, with special emphasis on the basics of image acquisition.
There will be time and equipment available for special techniques such as spectral imaging and FRAP. Participants are encouraged to provide their own samples and the instructors will be available to help in image acquisition optimization.
The workshop lectures will take place in 3-103 Nils Hasselmo Hall on the Minneapolis East Bank campus of the U. of Minnesota. Lectures will cover from the basics of fluorescence to the different instrumentation configurations and applications in the newest laser scanning confocal microscopes.
Hands on training and practice with user samples will take place in the Jackson Hall site of the UIC, with a Nikon TiE A1Rsi, an Olympus IX2 Fluoview 1000, two Olympus BX2 Fluoview 1000, a Nikon FN1 A1R MP, and a Zeiss Axio Observer Spinning Disk Confocal. In addition to the microscope systems, users will also be able to utilize UIC data analysis workstations. UIC staff will be available during the workshop for training and consultation.
- The PSF and resolution, NA and magnification, out of focus blur in thick samples and the confocal aperture. Point illumination.
- Fluorescence basics: The Jabloski diagram, spectra, Stokes’ shift, QY and brightness, quenching, photobleaching. Filters, sources and dye selection. Fluorescent proteins.
- Components and operation of a confocal microscope: what is what, how it works and the light path in a LSCM. Spectral acquisition.
- Components and operation of a confocal microscope: Spinning Disc confocal principles and applications.
- Acquiring a digital image: sampling and quantization. Nyquist sampling, resolution and aliasing. Bit depth.
- Confocal applications: fluorescence and reflectance, XY, XYZ, XYZT, FRAP, FRET (sensitized emission, acceptor photobleaching).
- Specimen preparation: labeling strategies and protocols.
- Image visualization, processing and analysis: 3D reconstruction and deconvolution (Elements and FIJI).
Participants will be divided in pairs for the hands-on part of the workshop (a total of 16 hours of scope time). Everyone in the class will work in a filter based CLSM (galvo and resonant), a spectral CLSM, and the SDCLM. UIC staff will train, demonstrate, and help attendees. The last day of the workshop (8 hours of scope time) is meant for attendees to work with their own samples, with help of UIC staff.
|Midwest Association of Core Directors (MWACD) (TCF Bank Stadium)||150||Wednesday, 16 October 2019 to Friday, 18 October 2019|
|UIC Nikon SoRa Workshop (CCRB)||30||Wednesday, 16 October 2019|
|Confocal Workshop (JH)||11||Tuesday, 16 July 2019 to Thursday, 18 July 2019|
|Confocal Workshop (JH)||12||Tuesday, 18 June 2019 to Thursday, 20 June 2019|
|Live Animal Image Acquisition and Analysis Workshop (JH)||4||Wednesday, 10 April 2019|
|Confocal Workshop (JH)||10||Tuesday, 11 July 2017 to Thursday, 13 July 2017|
|Confocal Workshop (JH)||10||Tuesday, 6 June to Thursday, 8 June 2017|
|Live Cell Workshop (CCRB)||8||Wednesday, 8 March 2017 to Thursday, 9 March 2017|
|Confocal Workshop (SH)||5||Tuesday, 13 December 2016 to Thursday, 15 December 2016|
|UIC & UMII Image Analysis Workshop (CCRB)||15||Thursday, 1 December 2016|
|Confocal Workshop (CCRB)||9||Tuesday, 1 November 2016 to Thursday, 3 November 2016|
|Confocal Workshop (JH)||24||Tuesday, 17 May 2016 to Thursday, 19 May 2016|
|UIC & UMII Workshop: An Introduction to Image Analysis (CCRB)||21||Friday, 23 October 2015|
|UIC & UMII Workshop: A Hands-On Introduction to FIJI (SH)||13||Wednesday, 5 August 2015|
|UIC & UMII Workshop: A Hands-On Introduction to FIJI (SH)||18||Wednesday, 29 July 2015|
|UIC & UMII Workshop: A Hands-On Introduction to FIJI (SH)||15||Wednesday, 22 July 2015|