Next generation In Situ technology
Spatially decode up to hundreds of genes simultaneously, within morphologically intact tissue samples at single cell resolution.
In Situ analysis is an innovative method of nucleic acid analysis performed directly in situ on the tissue section, preserving spatial information.
In Situ technology allows researchers to analyze either fresh/fixed frozen or FFPE samples and rapidly create single-cell gene expression maps of up to hundreds of genes. The technology ensures high performance (high specificity and high throughput) and reliable, reproducible data.
The highly specific padlock probes will hybridize to the RNA corresponding to your genes of interest.
Imaging cycles & Decoding
Barcodes are decoded from target-specific spots generated during the library preparation.
- Decoding starts with the hybridization of the 1st adapter probe pool to the spots and binding of the fluorescently labeled sequencing pool probes to the adapter probes.
- After imaging, the signal is removed to perform the next cycle.
Once all cycles are analyzed, each spot provides a unique fluorescent barcode identifying the targeted RNA.
Our In Situ technology results in a map of gene expression of selected genes while preserving tissue morphology.
In Situ analysis of 31 transcripts in fresh frozen human breast cancer samples using In Situ technology.