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MIBI

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Take the MIBI Challenge. Unlock your tissue’s story!

The UIC is pleased to announce the 2020- 2021 IONPath MIBI Seed Grant Awards. The projects selected will span a breadth of spatial proteomic applications that take advantage of the MIBIscope’s ability to map 40+ antibody targets in sectioned materials in the micron to 200nm resolution ranges. These one-year seed grants serve to facilitate innovative research solutions at the pilot scale which bring state-of-the-art technology to UMN Faculty, while developing expertise, external funding opportunities, and publications.

UICs’ MIBI Pilot Programs (ongoing)

  • UIC/Ionpath Seminar Speaker Support (on demand)

    • The UIC will provide introductory seminars to Departments, centers, and other research units to increase awareness of the newest proteomics imaging tools available to university researchers.

  • MIBI on the Horizon - provides seed funding to PIs to convene and prepare for major anticipated funding opportunities with proof of concept data. Funds can be used for staff and instrument time and for offsetting reagent costs. 

    • PI must supply a brief project description along with funding and publication plans. Up to $15,000. 

  • MIBI Updraft - enables researchers to reach a higher "altitude" by addressing immediate and short-term needs for publication or proof-of-concept for new direction. Funds can be used for staff and instrument time. Up to $5,000. 


The UIC will now accept seed grant applications to assist faculty in the preparation of publications or new directions (MIBI Updraft) and larger-scale research initiatives (MIBI on the Horizon).

MIBI on the Horizon

MIBI on the Horizon proposals should be no more than 3 pages in length. Collaborative efforts by multiple PIs will be prioritized. Priority will also be given to proposals that target federal funding at the level of center grants (NIH PXX and similar). All proposals should include application timeline along with specific aims that include how MIBI on the Horizon data will strengthen the proposal. Activities to be performed during the planning grant period such as workshops, symposia, and round-table discussions can be included as useful for the targeted RFP.

MIBI Updraft

MIBI Updraft proposals should be no more than 2 pages in length, and individual lab proposals will be prioritized. Proposals should clearly identify the experimental goals and timeline for results. Publications resulting from these efforts should be treated as a collaborative effort with UIC staff. Instrument and staff time will be covered and some UIC stocked reagents can be made available. Additional or custom reagents will need to be supplied by the investigator.


 

  • Proposals can be submitted at any time to the forms below, and will be evaluated upon receipt by an ad hoc committee. 
  • Proposals will be evaluated, in part, on how well the activities are likely to ready the research team for the targeted RFP or publication goals.
  • Funding for MIBI on the Horizon planned activities is capped at $15,000 per proposal and is for one year in duration.
  • Funding for MIBI Updraft planned activities is capped at $5,000 per proposal and is for six months in duration.
  • Funding is intended to support UIC expenses and administrative support to help with the actual proposal development/writing.  Funding is not intended for non-MIBI activities or to support salaries outside the UIC.

 

Multiplexed Ion Beam imaging (MIBI)

The MIBIscope System is a revolutionary imaging platform, enabling comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment. The MIBIscope allows researchers to visualize over 40 markers simultaneously with higher sensitivity, resolution, and throughput than existing methods.

MIBI

MIBI Technology is based on secondary ion mass spectrometry or SIMS. With SIMS, a primary ion beam is rastered across the surface of a sample, liberating reporter ions that are then simultaneously recorded on a pixel-by-pixel basis by Time-of-Flight detection. An ion beam, unlike a laser, enables resolution to be tuned over a broad range—in the case of the MIBIScope, from a few hundred nanometers to 1 micron. Once liberated, the reporter ions, or “secondary ions,” travel uninterrupted at super sonic speed from the sample to the detector, leading to fast acquisition and extraordinary sensitivity.

UIC Stocked Antibodies

Product Code Antibody Isotope
711301-100 b-tubulin (D3U1W) 113In
714401-100 CD11c (EP1347Y) 144Nd
716701-100 CD20 (L26) 167Er
715901-100 CD3 (D7A6E) 159Tb
715201-100 CD31 (EP3095)  152Sm
714301-100 CD4 (EPR6855)  143Nd
717501-100 CD45 (2B11 & PD7/26) 175Lu
715101-100 CD56 (MRQ-42) 151Eu
715601-100 CD68 (D4B9C) 156Gd
715801-100 CD8 (C8/144B) 158Gd
708901-100 dsDNA (35I9 DNA) 89Y
717602-100 HLA class 1 A, B, and C (EMR8-5) 176Yb
717201-100 HLA DR (EPR3692) 172Yb
716501-100 Keratin (AE1/AE3) 165Ho
717601-100 Na-K-ATPase (D4Y7E) 176Yb
716301-100 Vimentin (D21H3) 163Dy
Cell Type Phenotype
Tumor cells Keratin+, PAX5+, or SOX10+
Blood vessels CD31+
Fibroblasts Vimentin+
Immune cells CD45+
T cells CD3+
Helper T lymphocytes CD3+CD4+
Cytotoxic T lymphocytes CD3+CD8+
B cells CD20+
NK cells CD3-CD56+
Macrophages CD68+
M1 macrophage CD68+HLA-DR+
Dendritic cells CD11c+HLA-DR+

Protocols

MIBI Tissue Staining Protocol

Description: Protocol for tissue staining for multiplexed ion beam imaging.

Click here to Download

 

MIBItag Conjugation Protocol

Description: Protocol for labeling antibodies with MIBItags. Optimized for conjugation of 100 μg of antibody with one tube of metal-loaded MIBItag.

Click here to Download

MIBItracker

MIBItracker cloud-based repository lets you easily review slide and image data, and it’s easily accessible through a web browser. Users can customize the display for each image or image set including selecting channels for overlay, setting channel relative intensity, pixel smoothing, and channel pseudo-color. Images can also be exported as raw grayscale multi-layer TIFF files for subsequent analysis in third-party packages such as Fiji, HALO®, VisioPharm® and QuPath.