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Multiplexed Ion Beam imaging (MIBI)
The MIBIscope System is a revolutionary imaging platform, enabling comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment. The MIBIscope allows researchers to visualize over 40 markers simultaneously with higher sensitivity, resolution, and throughput than existing methods.
MIBI Technology is based on secondary ion mass spectrometry or SIMS. With SIMS, a primary ion beam is rastered across the surface of a sample, liberating reporter ions that are then simultaneously recorded on a pixel-by-pixel basis by Time-of-Flight detection. An ion beam, unlike a laser, enables resolution to be tuned over a broad range—in the case of the MIBIScope, from a few hundred nanometers to 1 micron. Once liberated, the reporter ions, or “secondary ions,” travel uninterrupted at super sonic speed from the sample to the detector, leading to fast acquisition and extraordinary sensitivity.
UIC Stocked Antibodies
|717501-100||CD45 (2B11 & PD7/26)||175Lu|
|708901-100||dsDNA (35I9 DNA)||89Y|
|717602-100||HLA class 1 A, B, and C (EMR8-5)||176Yb|
|717201-100||HLA DR (EPR3692)||172Yb|
|Tumor cells||Keratin+, PAX5+, or SOX10+|
|Helper T lymphocytes||CD3+CD4+|
|Cytotoxic T lymphocytes||CD3+CD8+|
MIBI Tissue Staining Protocol
Description: Protocol for tissue staining for multiplexed ion beam imaging.
MIBItag Conjugation Protocol
Description: Protocol for labeling antibodies with MIBItags. Optimized for conjugation of 100 μg of antibody with one tube of metal-loaded MIBItag.