The University of Minnesota's Medical Discovery Team (MDT) on Optical Imaging and Brain Science is a multi-disciplinary effort focused on mapping the detailed circuits that underlie sensation, perception and complex behaviors in the developing and mature brain. State-of-the-art optical imaging and optical stimulation techniques that provide sub-micron spatial resolution will be used in experimental model systems of health, injury and disease. These techniques include two-photon and three-photon imaging and holographic stimulation of individual cells using two-photon optogenetics. Recent advances in optics help preserve the high spatial resolution even when examining multiple brain regions simultaneously. Thus, the activity from large ensembles of cells will provide near complete wiring diagrams underlying specific behaviors in the healthy brain as well as in diseases such as Alzheimer's, epilepsy, autism, stroke, and vascular dementia. Moreover, to create synergy across the MDT’s, researchers in the Optical Imaging MDT may collaborate and lend their technologies to the Addiction MDT and the Nikon University Imaging Center (UIC) core facilitates.