Stem Cell Institute Research

Our faculty research initiatives are broadly based on the study of stem cells themselves, as well as the potential to use stem cells as models of human development and diseases, and for cell-based therapies. Learn more about our faculty members’ research.

Resources for Researchers

STEM CELL CORE

Provides resources and assistance for using stem cell and reprogramming technologies for research and translation.

LABORATORY FOR STEM CELL AUTOMATION

State-of-the-art facility for automating stem cell culture and technologies.

STEM CELL BUSINESS INCUBATOR

Infrastructure supporting early-stage stem cell businesses.

SHARED EQUIPMENT

CONFOCAL MICROSCOPE

This is a Zeiss Axioskop 2 upright microscope with a Biorad 2000 laser head with several objective options, including:

  • a Zeiss 20x Plan Apochromat (N.A. 0.6),
  • a Zeiss Plan Neofluar 40x oil phase (N.A. 1.3),
  • the world class Zeiss 63x oil Apochromat (N.A. 1.4), and
  • a Zeiss Plan Neofluar 100x oil phase (N.A. 1.3).

The scope has 3 lasers and is capable of excitation in a number of wavelengths:

  1. tunable 15 mW argon laser can excite at 514, 488, 476, and 457 nm;
  2. 10mW He/Ne that excites at 543nm
  3. Red 637 nm for APC. 

The PMTs are selectable with the appropriate mirror and band pass filter. Experiments can use all three PMTs in a single pass.

This instrument has the capability of the standard FITC, PE, Texas Red, PerCP, Cy2, Cy3, ToPro3, APC, and numerous other fluorophores.

Fluorophores: Filters and mirrors

Mirrors

PMT-1 Mirror PMT-2 Mirror PMT-3
100% Det-1
500 DCLPxR
560 DCLPxR
100% Det 2/3
100% Det-2
560 DCLPxR
650 DCLPxR
100% Det-3

Emission or band pass filter PMT-2
E 570 LP
E 600 LP
HQ660 LP (BLD)


Filters

Emission or band pass filter PMT-1 Emission or band pass filter PMT-2
HQ 442/45
HQ 476/48
HQ 485/30
D 488/10
HQ500 LP
HQ 515/30
HQ 530/60
HQ 515/30
HQ 550/60
HQ 545/40
E 570 LP
HQ 590/70 (BLD)
HQ 600/50 (BLD)
E 560 LP


Consultations on applications are available.

Training is required and is available by appointment.

For information, scheduling, or training please contact:

Sue Keirstead
612-626-2290
sakeir@umn.edu

INVERTED FLUORESCENCE MICROSCOPE

This microscope has a camera attached for taking pictures from culture dishes, plates, and flasks. The main scope is a Zeiss Axiovert 200m fitted with a matched Axiocam 4 mega-pixel camera. The scope is bright field, dark field, and phase capable and can take pictures of DAPI, FITC, PE, and PerCP in sequence with the automated setup.

The following objectives are available:

  • 2.5x Plan Neofluar (N.A. 0.075)
  • 10x A Plan Phase 1 var. (N.A. 0.25)
  • 20x long working distance A Plan (N.A. 0.3)
  • 40x Plan Neofluar phase 2 oil (N.A. 1.3), and
  • Long working distance 40x Achroplan phase 2 (N.A. 0.6).


With training the scope is easy to use. Training is by appointment only.

Contact for training:
Sue Keirstead 
612-626-2290
sakeir@umn.edu

Exiqon miR Inhibitor Libraries

The Stem Cell Institute has acquired miR inhibitor libraries from Exiqon as a shared resource for cell-based screens. The libraries contain 1972 miRCURY LNA human miR inhibitors and 1624 LNA mouse miR inhibitors.

Learn more about the technology

If you are interested in using this resource, please contact:

Michael Kyba
612-626-5869
kyba@umn.edu

Mandatory Lab Safety Training

It is important that everyone who enters a laboratory knows and understands certain safety procedures. There are different levels of required training:

  1. to have access to the McGuire Translational Research Facility (MTRF),
  2. to work in a laboratory, and 
  3. to work on projects requiring specialized knowledge.

Before commencing work in the lab, all lab personnel must complete:

  1. Bloodborne Pathogens Annual OSHA  Requirement (print a copy of your final quiz to bring along when you get your UCard verified for building access)
  2. Radiation Safety Orientation (print a copy of your final quiz to bring along when you get your UCard verified for building access)
  3. Introduction to Research Safety
  4. Chemical Safety
  5. Chemical Waste Management
  6. Lab-specific training from your principal investigator or laboratory supervisor within the first week of work/rotation. This training should be reported by enrolling and indicating completion of "SCI Lab-specific training" in the Training Hub.  Annual updates are required for this training and can also be reported in Training Hub.

Principal Investigators will assign additional training as required for projects working with:

Biohazardous material - Biological Safety in the Laboratory
Animals - Animal Use Training
Human samples - Bloodborne Pathogens: Advanced
Radioactive material - Radiation Safety for Radioactive Material Users
Controlled substances - Controlled Substances

Things change! If you find that any of these links are not working, please contact Sue Keirstead.