Regenerative Medicine Laboratory
Dr. Singh's laboratory focuses on strategies to repair and recover the failing heart. Our laboratory is immersed in studying the molecular regulation involved in heart regeneration and repair. Additionally, we utilize an array of cutting-edge technologies including iPSC-technology, gene manipulation (CRISPR/Cas9), nucleic acid-based therapy development, tissue engineering approaches to model cardiovascular disease for novel therapeutics. Our laboratory aims to integrate these tools to develop newer cardiovascular disease models for drug discovery. Additionally, we intend to generate mature cardiac tissue for understanding cardiovascular development and disease progression.
Our laboratory collaborates with multiple institutions, departments, and centers to achieve our goals. We aim to nurture the young generation with mentorship, logical thinking, and guidance.
See below for more details about the Regenerative Medicine Lab.
Developing technologies to cryopreserve cardiovascular systems.
Deciphering molecular networks involved in cardiomyocyte maturation and regeneration.
Deciphering mechanism of Hypertrophic Cardiomyopathy disease progression for novel drug discovery.
Nucleic Acid based approaches to promote heart regeneration.
Select Publications
Waldron, C.J., Kelly, L.A., Kawakami, Y., Abrahante Lloréns, J.E., Magli, A., Ogle, B.M.*, & Singh, B.N.* (2023). The Hh-GLI2-CKS1B network regulates the proliferation-to-maturation transition of human cardiomyocytes. (Accepted; Stem Cells Translational Medicine
Singh, B.N., Yucel, D., Garay, B.I., Tolkacheva, E.G., Kyba, M., Perlingeiro, R.C.R., van Berlo, J.H., & Ogle, B.M. (2023). Proliferation and Maturation: Janus and the Art of Cardiac Tissue Engineering. Circulation Research, 132(4), 519-540. PMID: 36795845.
Hsieh, J., Becklin, K., Givens, S., Komosa, E.R., Abrahante Llorens, J.E., Kamdar, F., Moriarity, B.S., Webber, B.R., Singh, B.N.*, & Ogle, B.M.* (2022). Myosin Heavy Chain Converter Domain Mutations Drive Early-Stage Changes in Extracellular Matrix Dynamics in Hypertrophic Cardiomyopathy. Frontiers in Stem Cell and Developmental Biology, 10, 894635. PMID: 35784482. * corresponding authors
Singh, B.N., Koyano-Nakagawa, N., Gong, W., Moskowitz, I.P., Weaver, C.V., Braunlin, E., Das, S., van Berlo, J.H., Garry, M.G.*, & Garry, D.J.* (2018). A conserved HH-Gli-Mycn network regulates heart regeneration from newt to human. Nature Communications, 9(1), 4237. PMID: 30315164.
Additional publications from Dr. Singh's lab can be found below.
Mohammad Shameem
Mohammad Shameem is a new member in the Singh Lab. He joined this lab as a Researcher at the University of Minnesota in Dec. 2023. His work is focused on understanding the mechanism of cardiovascular regeneration and disease modeling. Shameem has strong training in cell and molecular biology with an interest in discoveries having translational impact. He has been investigating mutations that give rise to cardiomyopathy in human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs).
Casey Kraft
Casey is a 4th year PhD student at the University of Minnesota in the Singh Lab and Bioheat and Mass Transfer (Bischof) Lab. His thesis work is focused on cryopreserving cardiac systems. In collaboration with the Finger Lab and Visible Heart (Iaizzo) Lab, he has worked on cryopreservation solution screening in rat hearts and CPA exposure profile optimization in skeletal muscle tissue. In the Singh Lab, his work is focused on developing a cardiac-specific cryopreservation solution for application in engineered heart tissue cryopreservation. Casey graduated with a B.S. in Chemical Engineering from CU Boulder and his undergraduate research involved protein engineering and biomaterials. At the University of Minnesota, he was selected as a trainee for the two-year NIH T32 Cardiovascular Engineering Program and his current career goal is professor (research or teaching). Casey’s non-research interests include: running, creative writing, board games, piano, and foosball.
Ahmed Sharara
Ahmed is a talented undergraduate student in the Department of Biomedical Engineering. He joined the Singh lab in 2023. His focus is on developing new techniques to induce in vitro maturation in hiPSC derived cardiomyocytes, through cell culture work, statistical RNA analysis, and 3D engineered tissue models. In the future, he plans to utilize these developed techniques to derive computational models of cardiomyocyte maturation to aid in disease and drug modeling.
The Singh lab aims to nurture young generation and researchers/explores in the field of cardiac systems. We are looking for highly motivated, and dedicated individuals to work closely with current lab members. Currently available positions are listed below.
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Volunteer Opportunity
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Research Scientist
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Post-Doctoral Position(s)
Please reach out to Dr. Singh with inquiries.
Contact
Laboratory Director:
Dr. Bhairab Singh
bnsingh@umn.edu
Bhairab N. Singh , PhD
Assistant Professor
Department of Rehabilitation Medicine, Division of PM&R