Daniel Garry Lab

 

Dan’s laboratory has a long-standing interest in regenerative and stem cell biology with a focus on the heart and skeletal muscle. In their studies of the heart and skeletal muscle, the Garry laboratory utilizes an array of technologies including gene disruption strategies, transgenesis, single cell genome analysis, gene editing (TALEN and CRISPR technologies), inducible ES/EB model systems, hiPSC technologies, FACS and other cellular, biochemical and molecular biological techniques.

In addition, their use of lower organisms such as zebrafish and newt, which are highly regenerative model systems have successfully uncovered critical regenerative factors. Human iPSCs are another important model for cardiovascular disease investigation for the lab. Using these technologies, the Garry lab was among the first to discover the molecular markers of stem cell populations that regulate critical networks during heart and skeletal muscle development and regeneration. For example, their studies have uncovered novel Ets and Forkhead transcription factors, microRNAs and signaling pathways that direct fate determination of stem cell populations. The manipulation of these pathways using chemical genetics and molecular technologies has provided a platform focused on rebuilding and repairing the injured heart and skeletal muscle.

Dan Garry faculty profile

Current Lab Projects

Regulatory Mechanisms of Endothelial Development and Regeneration

The overall goal of this project is to define the regulatory cascades that govern stem cells and progenitors to promote cardiovascular regeneration.

Transcriptional Regulation of Cardiac Progenitors

(AHA DeHaan Cardiac Myogenesis Award)
This project is part of the University of Minnesota AHA-Jon Holden DeHaan Cardiac Myogenesis Research Center: Moving myogenesis from molecules to man. The major objectives of this project are the focus on Nkx2-5 transcriptional networks and the definition of signaling pathways that promote a cardiomyocyte fate using hiPSC and adult cardiac progenitors.

Selected Publications

Full list of publications at Experts@Minnesota.

  1. Garry DJ, Das S, Gong W. Decoding DMD transcriptional networks using single-nucleus RNA sequencing. Proc Natl Acad Sci U S A. 2020 Dec 2;117(51):32192–4. doi: 10.1073/pnas.2022205117. Epub ahead of print. PMID: 33268493; PMCID: PMC7768731.
  2. Garry DJ, Maeng G, Garry MG. Foxk1 regulates cancer progression. Ann Transl Med. 2020 Sep;8(17):1041. doi: 10.21037/atm-2020-94. PMID: 33145260; PMCID: PMC7575999.
  3. Singh BN, Sierra-Pagan JE, Gong W, Das S, Theisen JWM, Skie E, Garry MG, Garry DJ. ETV2 (Ets Variant Transcription Factor 2)-Rhoj Cascade Regulates Endothelial Progenitor Cell Migration During Embryogenesis. Arterioscler Thromb Vasc Biol. 2020 Dec;40(12):2875-2890. doi: 10.1161/ATVBAHA.120.314488. Epub 2020 Oct 29. PMID: 33115267.
  4. Tao H, Lambert JP, Yung TM, Zhu M, Hahn NA, Li D, Lau K, Sturgeon K, Puviindran V, Zhang X, Gong W, Chen XX, Anderson G, Garry DJ, Henkelman RM, Sun Y, Iulianella A, Kawakami Y, Gingras AC, Hui CC, Hopyan S. IRX3/5 regulate mitotic chromatid segregation and limb bud shape. Development. 2020 Oct 5;147(19):dev180042. doi: 10.1242/dev.180042. PMID: 32907847; PMCID: PMC7561487.
  5. Garry DJ, Caplan AL, Garry MG. Chimeric Humanized Vasculature and Blood: The Intersection of Science and Ethics. Stem Cell Reports. 2020 Apr 14;14(4):538-540. doi: 10.1016/j.stemcr.2020.03.016. PMID: 32294412; PMCID: PMC7160389.
  6. Kamdar F, Das S, Gong W, Klaassen Kamdar A, Meyers TA, Shah P, Ervasti JM, Townsend D, Kamp TJ, Wu JC, Garry MG, Zhang J, Garry DJ. Stem Cell-Derived Cardiomyocytes and Beta-Adrenergic Receptor Blockade in Duchenne Muscular Dystrophy Cardiomyopathy. J Am Coll Cardiol. 2020 Mar 17;75(10):1159-1174. doi: 10.1016/j.jacc.2019.12.066. PMID: 32164890.
  7. Yellamilli A, Ren Y, McElmurry RT, Lambert JP, Gross P, Mohsin S, Houser SR, Elrod JW, Tolar J, Garry DJ, van Berlo JH. Abcg2-expressing side population cells contribute to cardiomyocyte renewal through fusion. FASEB J. 2020 Apr;34(4):5642-5657. doi: 10.1096/fj.201902105R. Epub 2020 Feb 25. PMID: 32100368; PMCID: PMC7136149.
  8. Das S, Koyano-Nakagawa N, Gafni O, Maeng G, Singh BN, Rasmussen T, Pan X, Choi KD, Mickelson D, Gong W, Pota P, Weaver CV, Kren S, Hanna JH, Yannopoulos D, Garry MG, Garry DJ. Generation of human endothelium in pig embryos deficient in ETV2. Nat Biotechnol. 2020 Mar;38(3):297-302. doi: 10.1038/s41587-019-0373-y. Epub 2020 Feb 24. PMID: 32094659.
  9. Garry DJ, Alexy T. Emerging Therapies for Dystrophic Cardiomyopathy. JACC Basic Transl Sci. 2019 Nov 25;4(7):792-794. doi: 10.1016/j.jacbts.2019.10.003. PMID: 31999276; PMCID: PMC6978550.
  10. Maeng G, Gong W, Das S, Yannopoulos D, Garry DJ, Garry MG. ETV2-null porcine embryos survive to post-implantation following incomplete enucleation. Reproduction. 2020 May;159(5):539-547. doi: 10.1530/REP-19-0382. PMID: 31990674; PMCID: PMC7409374.

Full list of publications at Experts@Minnesota.

Lab Members

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Faculty

 

Dan Garry

Daniel Garry, MD, PhD
Professor and Abbott Endowed Chair in Cardiovascular Sciences
garry@umn.edu

 

Cyprian Weaver

Cyprian Weaver, PhD, MS, BA
Associate Professor of Medicine
cyprian@umn.edu

 

Satya Das

Satyabrata Das, PhD
Assistant Professor of Medicine
sdas@umn.edu

 

Wuming Gong

Wuming Gong, PhD
Assistant Professor of Medicine
gongx030@umn.edu

 

Guenho Maeng
Assistant Professor of Medicine
gmaeng@umn.edu

Postdoctoral Associates

 

Joshua Thiessen

Joshua Theisen, MD, PhD
Post-Doctoral Associate
612-626-3035
theisenj@umn.edu

 

Javier Sierra-Pagan
Medical Student
sierr025@umn.edu

 

Matthew Jacobson
Student
jaco2000@umn.edu

 

Mateo Taddeini
Student
tadde013@umn.edu

Scientists

 

Erik Skie
Student Tech
skiex003@umn.edu

Jobs