Daniel Garry Lab
Dan’s laboratory has a long-standing interest in regenerative and stem cell biology with a focus on the heart and skeletal muscle. In their studies of the heart and skeletal muscle, the Garry laboratory utilizes an array of technologies including gene disruption strategies, transgenesis, single cell genome analysis, gene editing (TALEN and CRISPR technologies), inducible ES/EB model systems, hiPSC technologies, FACS and other cellular, biochemical and molecular biological techniques.
In addition, their use of lower organisms such as zebrafish and newt, which are highly regenerative model systems have successfully uncovered critical regenerative factors. Human iPSCs are another important model for cardiovascular disease investigation for the lab. Using these technologies, the Garry lab was among the first to discover the molecular markers of stem cell populations that regulate critical networks during heart and skeletal muscle development and regeneration. For example, their studies have uncovered novel Ets and Forkhead transcription factors, microRNAs and signaling pathways that direct fate determination of stem cell populations. The manipulation of these pathways using chemical genetics and molecular technologies has provided a platform focused on rebuilding and repairing the injured heart and skeletal muscle.
Regulatory Mechanisms of Endothelial Development and Regeneration
The overall goal of this project is to define the regulatory cascades that govern stem cells and progenitors to promote cardiovascular regeneration.
Transcriptional Regulation of Cardiac Progenitors
(AHA DeHaan Cardiac Myogenesis Award)
This project is part of the University of Minnesota AHA-Jon Holden DeHaan Cardiac Myogenesis Research Center: Moving myogenesis from molecules to man. The major objectives of this project are the focus on Nkx2-5 transcriptional networks and the definition of signaling pathways that promote a cardiomyocyte fate using hiPSC and adult cardiac progenitors.
Full list of publications at Experts@Minnesota.
- Garry DJ, Das S, Gong W. Decoding DMD transcriptional networks using single-nucleus RNA sequencing. Proc Natl Acad Sci U S A. 2020 Dec 2;117(51):32192–4. doi: 10.1073/pnas.2022205117. Epub ahead of print. PMID: 33268493; PMCID: PMC7768731.
- Garry DJ, Maeng G, Garry MG. Foxk1 regulates cancer progression. Ann Transl Med. 2020 Sep;8(17):1041. doi: 10.21037/atm-2020-94. PMID: 33145260; PMCID: PMC7575999.
- Singh BN, Sierra-Pagan JE, Gong W, Das S, Theisen JWM, Skie E, Garry MG, Garry DJ. ETV2 (Ets Variant Transcription Factor 2)-Rhoj Cascade Regulates Endothelial Progenitor Cell Migration During Embryogenesis. Arterioscler Thromb Vasc Biol. 2020 Dec;40(12):2875-2890. doi: 10.1161/ATVBAHA.120.314488. Epub 2020 Oct 29. PMID: 33115267.
- Tao H, Lambert JP, Yung TM, Zhu M, Hahn NA, Li D, Lau K, Sturgeon K, Puviindran V, Zhang X, Gong W, Chen XX, Anderson G, Garry DJ, Henkelman RM, Sun Y, Iulianella A, Kawakami Y, Gingras AC, Hui CC, Hopyan S. IRX3/5 regulate mitotic chromatid segregation and limb bud shape. Development. 2020 Oct 5;147(19):dev180042. doi: 10.1242/dev.180042. PMID: 32907847; PMCID: PMC7561487.
- Garry DJ, Caplan AL, Garry MG. Chimeric Humanized Vasculature and Blood: The Intersection of Science and Ethics. Stem Cell Reports. 2020 Apr 14;14(4):538-540. doi: 10.1016/j.stemcr.2020.03.016. PMID: 32294412; PMCID: PMC7160389.
- Kamdar F, Das S, Gong W, Klaassen Kamdar A, Meyers TA, Shah P, Ervasti JM, Townsend D, Kamp TJ, Wu JC, Garry MG, Zhang J, Garry DJ. Stem Cell-Derived Cardiomyocytes and Beta-Adrenergic Receptor Blockade in Duchenne Muscular Dystrophy Cardiomyopathy. J Am Coll Cardiol. 2020 Mar 17;75(10):1159-1174. doi: 10.1016/j.jacc.2019.12.066. PMID: 32164890.
- Yellamilli A, Ren Y, McElmurry RT, Lambert JP, Gross P, Mohsin S, Houser SR, Elrod JW, Tolar J, Garry DJ, van Berlo JH. Abcg2-expressing side population cells contribute to cardiomyocyte renewal through fusion. FASEB J. 2020 Apr;34(4):5642-5657. doi: 10.1096/fj.201902105R. Epub 2020 Feb 25. PMID: 32100368; PMCID: PMC7136149.
- Das S, Koyano-Nakagawa N, Gafni O, Maeng G, Singh BN, Rasmussen T, Pan X, Choi KD, Mickelson D, Gong W, Pota P, Weaver CV, Kren S, Hanna JH, Yannopoulos D, Garry MG, Garry DJ. Generation of human endothelium in pig embryos deficient in ETV2. Nat Biotechnol. 2020 Mar;38(3):297-302. doi: 10.1038/s41587-019-0373-y. Epub 2020 Feb 24. PMID: 32094659.
- Garry DJ, Alexy T. Emerging Therapies for Dystrophic Cardiomyopathy. JACC Basic Transl Sci. 2019 Nov 25;4(7):792-794. doi: 10.1016/j.jacbts.2019.10.003. PMID: 31999276; PMCID: PMC6978550.
- Maeng G, Gong W, Das S, Yannopoulos D, Garry DJ, Garry MG. ETV2-null porcine embryos survive to post-implantation following incomplete enucleation. Reproduction. 2020 May;159(5):539-547. doi: 10.1530/REP-19-0382. PMID: 31990674; PMCID: PMC7409374.
Faculty
Daniel Garry, MD, PhD
Professor and Abbott Endowed Chair in Cardiovascular Sciences
garry@umn.edu
Cyprian Weaver, PhD, MS, BA
Associate Professor of Medicine
cyprian@umn.edu
Satyabrata Das, PhD
Assistant Professor of Medicine
sdas@umn.edu
Wuming Gong, PhD
Assistant Professor of Medicine
gongx030@umn.edu
Guenho Maeng
Assistant Professor of Medicine
gmaeng@umn.edu
Postdoctoral Associates
Yong-ho Choe, Post-Doctoral Associate
Joined the Lab: May, 2022
Education: 2021 Ph.D. - Veterinary Obstetrics - Gyeongsang National University (Republic of Korea)
Research Interests: Pig cloning using somatic cell nuclear transfer (SCNT). Generation of Human-Pig chimera using Blastocyst complementation technology.
Accomplishments: Abstract for oral presentation accepted for 2023 CardioPalooza at the University of Minnesota.
Contact: choe0038@umn.edu
Joshua Theisen, MD, PhD, Post-Doctoral Associate
Contact: theisenj@umn.edu
Scientists
Erik Skie
Student Tech
skiex003@umn.edu
Other Lab Members
Young Geun Choi, PhD Student
Joined the Lab: February, 2023
Education:
- 2022 M.S. - Biomedical Engineering - Johns Hopkins University
- 2017 B.S. - Chemical Engineering - UCLA
Research Interests: Cellular reprogramming, Cardiovascular system
Contact: choi0889@umn.edu
Javier Sierra-Pagan, Medical Student
Contact: sierr025@umn.edu
Matthew Jacobson, Student
Contact: jaco2000@umn.edu
Mateo Taddeini, Student
Contact: tadde013@umn.edu