Our laboratory has a long-term interest in understanding the molecular mechanisms controlling lineage-specific differentiation of pluripotent stem cells (i.e. embryonic and adult reprogrammed stem cells), and applying this information to efficiently generate tissue-specific stem/progenitor cells endowed with in vivo regenerative potential. Our ultimate goal is to develop safe strategies to enable their future therapeutic application.
- Current Lab Projects
- Selected Publications
- Lab Members
- Lab Life
"Creating New Muscle by Using Stem Cells" (Duchenne UK)
"UMN Researchers Discover How Three-Dimensional Organization of the Genome Regulates Cell Differentiation" (UMN Med School)
"UMN Medical School Study Provides New Insight Into the Use of Cell Replacement Therapies to Treat Muscular Dystrophies" (UMN Med School)
"Feature Researcher: Rita Perlingeiro" (Stem Cell Institute)
"U Of M Makes Muscular Dystrophy Breakthrough" (WCCO video)
"U researchers use stem cells to regenerate muscles in diseased mice" (MN Public Radio)
"U of M researchers develop new muscular dystrophy treatment approach using human stem cells" (Science NewsLine)
"U of M researchers utilize genetically corrected stem cells to spark muscle regeneration"
Current Lab Projects
- Transcriptional mechanisms and signaling pathways controlling mesodermal cell fate
- Strategies to enable translational application of iPS cells to treat muscular dystrophies
- Genetic correction of disease- and patient-specific iPS cells
- Dissecting the mechanisms associated with stem cell self-renewal and long-term engraftment
Full list of publications at Experts@Minnesota.
- Magli A, Baik J, Kwak IY, Stafford D, Swanson SA, Stewart R, Thomson JA, Garry DJ and Perlingeiro RCR, (2019) Time-dependent Pax3-mediated chromatin remodeling and cooperation with Six4 and Tead2 specify the skeletal myogenic lineage in developing mesoderm. PLOS Biology, 17(2): e3000153. https://doi.org/10.1371/journal.pbio.3000153.
- Incitti T, Magli A, Darabi R, Arpke RJ, Kyba M and Perlingeiro RCR, (2019) Pluripotent stem cell-derived myogenic progenitors remodel their molecular signature upon in vivo engraftment. PNAS, Mar 2019, 116 (10) 4346-4351; DOI: 10.1073/pnas.1808303116.
- Borges L, Oliveira VKP, Baik J, Bendall SC and Perlingeiro RCR, (2018) Serial transplantation reveals a critical role for endoglin in hematopoietic stem cell quiescence. Blood, 133:688-696; doi: https://doi.org/10.1182/blood-2018-09-874677.
- Mondragon-Gonzalez R, Perlingeiro RCR, (2018) Recapitulating muscle disease phenotypes with myotonic dystrophy 1 iPS cells: a tool for disease modeling and drug discovery. Dis Model Mech. 2018 Jun 13. pii: dmm.034728. doi: 10.1242/dmm.034728. [Epub ahead of print]
- Magli A and Perlingeiro RCR, (2017) Myogenic Progenitor Specification from Pluripotent Stem Cells. Invited Review for Seminars in Cell and Developmental Biology, 2017:72:87-98.
- Kim, J, Oliveira VKP, Yamamoto A, Perlingeiro RCR, (2017) Generation of Skeletal Myogenic Progenitors from Human Pluripotent Stem Cells Using Non-Viral Delivery of Minicircle DNA. Stem Cell Research 23:87-94. PMCID: PMC5582001.
- Magli A, Incitti T, Kiley J, Swanson SA, Darabi R, Rinaldi F, Selvaraj S, Yamamoto A, Tolar J, Yuan C, Stewart R, Thomson JA, Perlingeiro RCR, (2017) PAX7 Targets, CD54, Integrin α9β1, and SDC2, Allow Isolation of Human ESC/iPSC-Derived Myogenic Progenitors. Cell Reports 19:2867-2877. PMID: 28658631.
- Kim J, Magli A, Chan SSK, Oliveira VKP, Wu J, Darabi R, Kyba M, Perlingeiro RCR, (2017) Expansion and Purification Are Critical for the Therapeutic Application of Pluripotent Stem Cell-Derived Myogenic Progrenitors. Stem Cell Reports 9:12-22. PMID: 28528701.
- Dourado KC, Baik J, Oliveira VKP, Beltrame M, Yamamoto A, Theuer, CP, Figueiredo CAV, Verneris MR & Perlingeiro RCR, (2017) Endoglin: a Novel Target for Therapeutic Intervention in Acute Leukemias Revealed in Xenograft Mouse Models. Blood 129:2526-2536. PMID: 28351936.
- Baik J, Magli A, Tahara N, Swanson SA, Borges L, Koyano-Nakagawa N, Stewart R, Garry DG, Kawakami Y, Thomson JA & Perlingeiro RCR, (2016) Endoglin Integrates BMP and Wnt Signaling to Induce Hematopoiesis through JDP2. Nature Communications 7:13101. PMID: 27713415.
- Carrió E, Magli A, Muñoz M, Peinado MA, Perlingeiro RCR, & Suelves M, (2016) Muscle cell identity requires Pax7-mediated lineage-specific DNA demethylation. BMC Biology 14:30. PMID: 27075038.
- Filareto A, Rinaldi F, Arpke RW, Darabi R, Belanto JJ, Toso EA, Miller AZ, Ervasti JM, McIvor RS, Kyba M, & Perlingeiro RCR, (2015) Pax3-induced expansion enables the genetic correction of dystrophic satellite cells. Skeletal Muscle 5:36. PMID: 26504514.
- McCullagh KJA & Perlingeiro RCR, (2015) Coaxing stem cells for skeletal muscle repair. Advanced Drug Delivery Reviews 84:198-207. PMID: 25049085.
- Skoglund G, Laine J, Darabi R, Fournier E, Perlingeiro RCR, Tabti N, (2014) Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. Proc Natl Acad Sci USA 111:8275-8280.
- Magli A, Schnettler E, Swanson SA, Borges L, Hoffman K, Stewart R, Thomson JA, Perlingeiro RCR, (2014) Pax3 and Tbx5 specify whether PDGFRα+ cells assume skeletal or cardiac muscle fate in differentiating ES cells. Stem Cells 32:2072-2083. PMID: 24677751.
- Filareto A, Parker S, Darabi R, Borges L, Iacovino M, Schaaf T, Mayerhofer T, Chamberlain JS, Ervasti JM, McIvor RS, Kyba M, Perlingeiro RCR, (2013) An Ex Vivo Gene Therapy Approach to Treat Muscular Dystrophy Using Inducible Pluripotent Stem Cells. Nat Communication 4:1549. PMID: 23462992.
- Magli A, Schnettler E, Rinaldi F, Brember P, Perlingeiro RCR, (2013) Functional Dissection of Pax3 in Paraxial Mesoderm Development and Myogenesis. Stem Cells 31(1):59-60. PMID: 23081715.
- Borges L, Iacovino M, Mayerhofer T, Koyano-Nakagawa N, Baik J, Garry DJ, Kyba M, Letarte M, Perlingeiro RCR, (2012) A Critical Role for Endoglin in the Emergence of Blood During Embryonic Development. Blood 119(23):5417-5428. PMID: 22535663.
- Darabi R, Arpke RW, Irion S, Dimos JT, Grskovic M, Kyba M, Perlingeiro RCR, (2012) Human ES- and iPS-Derived Myogenic Progenitors Restore DYSTROPHIN and Improve Contractility upon Transplantation in Dystrophic Mice. Cell Stem Cell 10(5):610-9. PMID:22560081.
- Darabi R, Santos FNC, Filareto A, Pan W, Koene R, Rudnicki M, Kyba M, Perlingeiro RCR, (2011) Assessment of the Myogenic Stem Cell Compartment Following Transplantation of Pax3/Pax7-Induced Embryonic Stem Cell-Derived Progenitor. Stem Cells 29:777-790. PMID: 21374762.
- Perlingeiro RCR, (2007) Endoglin is required for hemangioblast and early hematopoietic development. Development 134:3041-3048. PMID: 17634194.
Full list of publications at Experts@Minnesota
Alessandro Magli, Ph.D.
Position: Assistant Professor of Medicine
Joined the Lab: April 2009
Education: 2008 PhD - Molecular & Regenerative Medicine - University of Modena and Reggio Emilia (Italy)
Research Interests: Molecular Mechanisms behind Cell Differentiation, specifically Various Transcription Factor Contribution to Cell Fate Determination
2015 - Recipient of the Regenerative Medicine Minnesota Educational Grant
2010 - FASEB Summer Research Conferences - Skeletal Muscle Satellite and Stem Cells. July 18 – 23 - Carefree, AZ, USA. Magli A. (oral presentation). "Molecular analysis of Pax3-dependent gene regulation in the embryonic skeletal myogenic program."
2010 - Visscher symposium – June 3rd, Minneapolis (MN) USA Magli A (oral presentation). "Insights on mesoderm formation: a new role for Pax3."
Karim Azzag, Ph.D.
Position: Post-Doctoral Fellow
Joined the lab: January 2018
Education: 2017 PhD, cellular biology, Montpellier II University
Research Interests: Molecular Mechanisms behind Cell Differentiation and iPSC-based cell therapy for muscular dystrophies
June Baik, Ph.D.
Position: Post-Doctoral Fellow
Joined the Lab: November 2007
Education: 2015 Ph.D. – Molecular, Cellular, Developmental Biology and Genetics – University of Minnesota
Research Interests: The role of endoglin in leukemia
2017-2019 Recipient, Hartwell Foundation Fellowship, Hartwell Foundation, United states
2017 Recipient, Hematology Research Training Program (2T32HL007062), NIH Heart, Lung, and Blood Institute (NHLBI), United States
2014-2016 Recipient, PCBC Skills and Development Trainee Award (UMN Hub), and PCBC Jump Start Grant, NIH Heart, Lung, and Blood Institute (NHLBI), United States
2014-2015 Recipient, Doctoral Dissertation Fellowship, University of Minnesota, United States
2013-2015 Recipient, MN Muscle Training Grant (5T32AR007612-12), National Institutes of Health (NIH), United State
Neha Dhoke, Ph.D.
Position: Post-Doctoral Associate
Joined the Lab: December 2017
Education: PhD 2017 Stem Cell Biology and Regenerative Medicine, CSIR-Indian Institute of Chemical Technology
Research Interests: Targeting dystroglycanopathies using pluripotent stem cell-derived myogeneic progenitors
Accomplishments: Abstract for oral presentation accepted for 2020 annual meeting of the American Society for Gene and Cell Therapy
Aline Miyoko Sakaguchi Yamashita, Ph.D.
Position: Post-Doctoral Associate
Joined the Lab: February 2021
Education: 2019 PhD Biological Chemistry, Federal University of Rio de Janeiro
Research Interests: Molecular mechanisms during maturation of Pluripotent Stem Cell derived myogenic progenitors
Position: Lab Manager, Researcher 2
Joined the Lab: April 2013
Education: 2004 BS – Biology – University of Minnesota
Research Interests: Cardiology and Muscular Dystrophy, previous research included vascular biology and sickle cell anemia
Position: Researcher 1
Joined the lab: March 2018
Education: M.S., Biology and Computer Science, Bowling Green State University
Research Interests: In vivo studies of Muscular Dystrophies
Position: M.D.-Ph.D. Student (MSTP)
Joined the Lab: July 2018
Education: University of California, Berkeley, B.A. Molecular and Cell Biology-Neurobiology
Research Interests: My primary scientific interest is to understand the mechanisms driving cellular degeneration with an emphasis on early detection, rescue, and prevention. One of my goals is to use patient-derived and CRISPR-Cas9-corrected iPSCs in the context of striated muscle differentiation (diseased and normal stage), and the neuromuscular junction (NMJ) as a platform for drug screening, and to unravel biological processes that might not be easily accessible in vivo. My ultimate goal is to develop an in vitro model of Duchenne and Becker muscular dystrophies using hiPSCs: skeletal muscle, motor neurons, and cardiomyocytes.
Ruth L. Kirschstein National Research Service Award F30 Fellowship, NHLBI, March 5, 2020
Sping and Ying Ngoh Lin Award, March 7th, 2020
Carolina Ortiz, M.S.
Position: PhD Candidate in Integrative Biology and Physiology
Joined the Lab: October 2013
Education: B.Sc. Biology, Universidad de Costa Rica, San José, Costa Rica 2012
M.S. in Stem Cell Biology, University of Minnesota, 2015
Candidate to Professional Master's degree in Project Management, Universidad Latina, San José, Costa Rica
Research Interests: In vitro modeling of muscular dystrophies
2019 Award for Best Poster, Dept. of Medicine Hebbel Research Day, University of Minnesota, MN, United States
2015-2019 PINN fellowship, Ministry of Science and Technology, Costa Rica
2016 Allan Hemingway Scholarship, Integrated Biology and Physiology, University of Minnesota, MN, United States
2014 2nd Annual Regional Regeneration Symposium, November 2014, oral presentation: "In vitro modeling of Duchenne Muscular Dystrophy using patient-specific iPS cell-derived skeletal muscle derivatives." University of Minnesota, MN, United States
Position: Graduate Student
Joined the Lab: March 2018
Education: BS Biology, University of Wisconsin-Madison
Graduate student in Molecular, Cellular, Developmental Biology and Genetics, University of Minnesota, Expected graduation-2022
Research Interests: In vitro modeling of Duchene and Becker muscular dystrophies using skeletal muscles from patient-derived iPSCs.
Joined the lab: September 2019
Education: Expected Graduation Date 2022: B.S., Microbiology, University of Minnesota
Research Interests: Muscular Dystrophy