Perlingeiro Lab
Our laboratory has a long-term interest in understanding the molecular mechanisms controlling lineage-specific differentiation of pluripotent stem cells (i.e. embryonic and adult reprogrammed stem cells), and applying this information to efficiently generate tissue-specific stem/progenitor cells endowed with in vivo regenerative potential. Our ultimate goal is to develop safe strategies to enable their future therapeutic application.
- Media
- Current Lab Projects
- Selected Publications
- Lab Members
- Lab Life
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Media
"Creating New Muscle by Using Stem Cells" (Duchenne UK)
"UMN Researchers Discover How Three-Dimensional Organization of the Genome Regulates Cell Differentiation" (UMN Med School)
"UMN Medical School Study Provides New Insight Into the Use of Cell Replacement Therapies to Treat Muscular Dystrophies" (UMN Med School)
"Feature Researcher: Rita Perlingeiro" (Stem Cell Institute)
"U Of M Makes Muscular Dystrophy Breakthrough" (WCCO video)
"U researchers use stem cells to regenerate muscles in diseased mice" (MN Public Radio)
"U of M researchers develop new muscular dystrophy treatment approach using human stem cells" (Science NewsLine)
"U of M researchers utilize genetically corrected stem cells to spark muscle regeneration" -
Current Lab Projects
- Transcriptional mechanisms and signaling pathways controlling mesodermal cell fate
- Strategies to enable translational application of iPS cells to treat muscular dystrophies
- Genetic correction of disease- and patient-specific iPS cells
- Dissecting the mechanisms associated with stem cell self-renewal and long-term engraftment
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Selected Publications
Full list of publications at Experts@Minnesota.
- Dhoke N, Kim H, Selvaraj S, Oliveira NAJ, Azzag K, Tungtur S, Ortiz-Cordero C, Kiley J, Lu QL, Bang A, & Perlingeiro RCR, (2021), “A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy”. Cell Reports. (in press).
- Ortiz-Cordero C, Magli A, Dhoke N, Kuebler T, Selvaraj S, Oliveira NA, Zhou H, Sham YY, Bang AG, & Perlingeiro RCR, (2021), “NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes”. Elife, 2021 10:e65443. PMCID: PMC7924940
- Ortiz-Cordero C, Azzag K, & Perlingeiro RCR, (2021), “Fukutin-Related Protein: from Pathology to Treatments”. Trends in Cell Biology, 31:197-210. PMID: 33272829 (cover article).
- Kim H, Selvaraj S, Kiley J, Azzag K, Garay BI, & Perlingeiro RCR, (2021), “Genomic safe harbor expression of PAX7 for the generation of engraftable myogenic progenitors”. Stem Cell Reports, 16:10-19.PMCID: PMC7815936
- BaikJ, Felices M, Yingst A, Theuer, CP, Verneris MR, Miller JS, & Perlingeiro RCR,(2020), “Therapeutic effect of TRC105 and decitabine combination in AML xenografts”. Heliyon, 6(10):e05242. PMCID: PMC7566100.
- Incitti T, Magli A, Jenkins J, Lin K, Yamamoto A and Perlingeiro RCR, (2020), “Pluripotent stem cell-derived skeletal muscle fibers preferentially express oxidative myosin heavy-chain isoforms: new implications for Duchenne Muscular Dystrophy”. Skeletal Muscle, 10(1):17. PMCID: PMC7268645
- Azzag K, Ortiz-Cordero C, Oliveira NAJ, Magli A, Selvaraj S, Tungtur S, Upchurch W, Iaizzo PA, Lu QL and Perlingeiro RCR, (2020) “Efficient Engraftment of Pluripotent Stem Cell-Derived Myogenic Progenitors in a Novel Immunodeficient Mouse Model of Limb Girdle Muscular Dystrophy 2I”. Skeletal Muscle, 10(1):10. PMCID: PMC7175515.
- Selvaraj S, Mondragon-Gonzalez R, Xu B, Magli A, Kim H, Lainé J, Kiley J, McKee H, Rinaldi F, Aho J, Tabti N, Shen W, & Perlingeiro RCR, (2019) “Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes”. eLIFE, 8. pii: e47970. PMCID: PMC6845233.
- Selvaraj S, Dhoke N, Kiley J, Aierdi AJM, Mondragon-Gonzalez R, Killeen G, Oliveira VKP, Tungtur S, Munain AL & Perlingeiro RCR, (2019) “Gene Correction of Limb Girdle Muscular Dystrophy Type 2A Patient-Specific iPS Cells for the Development of Targeted Autologous Cell Therapy”. Molecular Therapy,27:2147-2157. PMCID: PMC6904833.
- Selvaraj S, Kyba M & Perlingeiro RCR, (2019) “Pluripotent Stem Cell-Based Therapeutics for Muscular Dystrophies” Trends in Molecular Medicine. 25:803-816. PMCID: PMC6721995. (cover article)
- Mondragon-Gonzalez R, Azzag K, Selvaraj S, Yamamoto A & Perlingeiro RCR, (2019) “Transplantation studies reveal internuclear transfer of toxic RNA in engrafted muscles of myotonic dystrophy 1 mice”. eBioMedicine. 47:553-562. PMCID: PMC6796515.
- Magli A, Baik J, Pota P, Ortiz Cordero C, Kwak IY, Garry DJ, Love PE, Dynlacht BD and Perlingeiro RCR, (2019) “Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification”. Nature Communications, 10:2316. PMCID: PMC6534668.
Full list of publications at Experts@Minnesota
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Lab Members
Karim Azzag, Ph.D.Position: Post-Doctoral Fellow
Joined the lab: January 2018
Education: 2017 PhD, cellular biology, Montpellier II University
Research Interests: Molecular Mechanisms behind Cell Differentiation and iPSC-based cell therapy for muscular dystrophies
Contact: kazzag@umn.edu
June Baik, Ph.D.Position: Post-Doctoral Fellow
Joined the Lab: November 2007
Education: 2015 Ph.D. – Molecular, Cellular, Developmental Biology and Genetics – University of Minnesota
Research Interests: The role of endoglin in leukemia
Accomplishments:
- 2021 Recipient, T32 Training Program in Cardiac Innovation, NIH Heart, Lung, and Blood Institute (NHLBI), United States
- 2017-2019 Recipient, Hartwell Foundation Fellowship, Hartwell Foundation, United states
- 2017 Recipient, Hematology Research Training Program (2T32HL007062), NIH Heart, Lung, and Blood Institute (NHLBI), United States
- 2014-2016 Recipient, PCBC Skills and Development Trainee Award (UMN Hub), and PCBC Jump Start Grant, NIH Heart, Lung, and Blood Institute (NHLBI), United States
- 2014-2015 Recipient, Doctoral Dissertation Fellowship, University of Minnesota, United States
- 2013-2015 Recipient, MN Muscle Training Grant (5T32AR007612-12), National Institutes of Health (NIH), United State
Sarah Crist, Ph.D.Position: Post-Doctoral Fellow
Joined the Lab: November 2021
Education:
2021 Ph.D. – Molecular and Cellular Biology – University of Washington/Fred Hutchinson CRC
2013 B.S. – Biology – Haverford CollegeResearch Interests: Exploring the active role of the skeletal muscle microenvironment on iPax3 myogenic progenitor maturation, with particular interest in both endogenous extracellular matrix constituents and novel deposition by the transplanted myogenic progenitors.
Accomplishments:
2022 - Harold M. Weintraub Graduate Student AwardContact: crist156@umn.edu
Neha Dhoke, Ph.D.Position: Post-Doctoral Associate
Joined the Lab: December 2017
Education: PhD 2017 Stem Cell Biology and Regenerative Medicine, CSIR-Indian Institute of Chemical Technology
Research Interests: Targeting dystroglycanopathies using pluripotent stem cell-derived myogeneic progenitors
Accomplishments: Abstract for oral presentation accepted for 2020 annual meeting of the American Society for Gene and Cell Therapy
Contact: ndhoke@umn.edu
Phablo AbreuPosition: Post-Doctoral Associate
Joined the Lab: July 2021
Education: 2015 Ph.D. - Physiology - University of São Paulo (USP)
Research Interests: Metabolism, stem cells, and skeletal muscle regeneration
Contact: abreu030@umn.edu
Aline Miyoko Sakaguchi Yamashita, Ph.D.Position: Post-Doctoral Associate
Joined the Lab: February 2021
Education: 2019 PhD Biological Chemistry, Federal University of Rio de Janeiro
Research Interests: Molecular mechanisms during maturation of Pluripotent Stem Cell derived myogenic progenitors
Contact: sakag012@umn.edu
Jim KileyPosition: Lab Manager, Researcher 2
Joined the Lab: April 2013
Education: 2004 BS – Biology – University of Minnesota
Research Interests: Cardiology and Muscular Dystrophy, previous research included vascular biology and sickle cell anemia
Contact: kile0003@umn.edu
Bayardo Garay Position: M.D.-Ph.D. Student (MSTP)
Joined the Lab: July 2018
Education: University of California, Berkeley, B.A. Molecular and Cell Biology-Neurobiology
Research Interests: My primary scientific interest is to understand the mechanisms driving cellular degeneration with an emphasis on early detection, rescue, and prevention. One of my goals is to use patient-derived and CRISPR-Cas9-corrected iPSCs in the context of striated muscle differentiation (diseased and normal stage), and the neuromuscular junction (NMJ) as a platform for drug screening, and to unravel biological processes that might not be easily accessible in vivo. My ultimate goal is to develop an in vitro model of Duchenne and Becker muscular dystrophies using hiPSCs: skeletal muscle, motor neurons, and cardiomyocytes.
Accomplishments:
Ruth L. Kirschstein National Research Service Award F30 Fellowship, NHLBI, March 5, 2020
Sping and Ying Ngoh Lin Award, March 7th, 2020Contact: garay034@umn.edu
Chiemelie OnyebuPosition: Undergraduate
Joined the lab: March 2022
Education: Expected Graduation Date May 2023: B.S.E., Chemical Engineering, University of Minnesota
Research Interests: Improving muscle engraftment of transplanted PSC-derived myogenic progenitors through tissue engineering.
Contact: onyeb002@umn.edu
Peter SalamaPosition: Undergraduate
Joined the lab: January 2020
Education: Expected Graduation Date 2022: B.S., Neuroscience and History, University of Minnesota
Research Interests: Pluripotent Stem Cell Derived myogenic progenitors.
Contact: salam056@umn.edu
Aisha ShaikhPosition: Undergraduate
Joined the lab: February 2022
Education: Expected Graduation Date May, 2023: B.S., Biology with Islamic Studies Minor, University of Minnesota
Research Interests: Transcriptional networks and physiologic adaptations regarding Duchenne Muscular Dystrophy.
Contact: shaik096@umn.edu
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Lab Life