Perlingeiro Lab
Our laboratory has a long-term interest in understanding the molecular mechanisms controlling lineage-specific differentiation of pluripotent stem cells (i.e. embryonic and adult reprogrammed stem cells), and applying this information to efficiently generate tissue-specific stem/progenitor cells endowed with in vivo regenerative potential. Our ultimate goal is to develop safe strategies to enable their future therapeutic application.
- Media
- Current Lab Projects
- Selected Publications
- Lab Members
- Lab Life
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Media
"Creating New Muscle by Using Stem Cells" (Duchenne UK)
"UMN Researchers Discover How Three-Dimensional Organization of the Genome Regulates Cell Differentiation" (UMN Med School)
"UMN Medical School Study Provides New Insight Into the Use of Cell Replacement Therapies to Treat Muscular Dystrophies" (UMN Med School)
"Feature Researcher: Rita Perlingeiro" (Stem Cell Institute)
"U Of M Makes Muscular Dystrophy Breakthrough" (WCCO video)
"U researchers use stem cells to regenerate muscles in diseased mice" (MN Public Radio)
"U of M researchers develop new muscular dystrophy treatment approach using human stem cells" (Science NewsLine)
"U of M researchers utilize genetically corrected stem cells to spark muscle regeneration"Webinars, Podcasts, and Other Streaming Talks
May 23, 2022: LGMD Global Advocacy Summit, Zoom presentation
April 2021: Muscular Dystrophy Research Day, Minneapolis, MN, Zoom presentation
Feb 21, 2021: “iPSC-Derived Myogenic Progenitors” (Culturing knowledge in stem cell research; Ep 141 Featuring Dr. Rita Perlingeiro)
Dec 21, 2020: Webinar on Muscle Regeneration, French FSHD Society, Zoom presentation -
Current Lab Projects
- Dissecting the molecular mechanisms controlling in vitro and in vivo maturation of PSC-derivatives
- Understanding how the muscle environment influences the engraftment of myogenic progenitors
- Preclinical studies of PSC-derived myogenic progenitors to enable clinical translation
- Disease modeling of DMD and BMD using patient-specific iPSC-derived skeletal and cardiac muscle cells
- Understanding the impact of HLA mismatch on muscle engraftment
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Selected Publications
Full list of publications at Experts@Minnesota or PubMed.
- Garay BI, Givens S, Stanis N, Magli A, Yücel D, Abrahante JE, Goloviznina NA, Soliman HAN, Dhoke NR, Baik J, Kyba M, van Berlo JH, Ogle B, & Perlingeiro RCR, (2022) Inhibition of mitogen-activated protein kinase pathway enhances maturation of human iPSC-derived cardiomyocytes. Stem Cell Reports. in press.
- Kim H, & Perlingeiro RCR, (2022) Generation of human myogenic progenitors from pluripotent stem cells for in vivo regeneration. Cellular and Molecular Life Sciences. 8;79(8):406. doi: 10.1007/s00018-022-04434-8. PMCID: PMC9270264.
- Ortiz-Cordero C, Bincoletto, C, Dhoke N, Selvaraj S, Magli A, Zhou H, Kim D-H, Bang AG, & Perlingeiro RCR, (2021), Defective autophagy and increased apoptosis contribute toward the pathogenesis of FKRP-associated muscular dystrophies. Stem Cell Reports. 16(11):2752-2767. PMCID: PMC8581053.
- Dhoke N, Kim H, Selvaraj S, Oliveira NAJ, Azzag K, Tungtur S, Ortiz-Cordero C, Kiley J, Lu QL, Bang A, & Perlingeiro RCR, (2021), A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapy. Cell Reports. 36(2):109360. PMCID: PMC8327854.
- Ortiz-Cordero C, Magli A, Dhoke N, Kuebler T, Selvaraj S, Oliveira NA, Zhou H, Sham YY, Bang AG, & Perlingeiro RCR, (2021), “NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes”. Elife, 2021 10:e65443. PMCID: PMC7924940
- Ortiz-Cordero C, Azzag K, & Perlingeiro RCR, (2021), “Fukutin-Related Protein: from Pathology to Treatments”. Trends in Cell Biology, 31:197-210. PMID: 33272829 (cover article).
- Kim H, Selvaraj S, Kiley J, Azzag K, Garay BI, & Perlingeiro RCR, (2021), “Genomic safe harbor expression of PAX7 for the generation of engraftable myogenic progenitors”. Stem Cell Reports, 16:10-19.PMCID: PMC7815936
- Baik J, Felices M, Yingst A, Theuer, CP, Verneris MR, Miller JS, & Perlingeiro RCR,(2020), “Therapeutic effect of TRC105 and decitabine combination in AML xenografts”. Heliyon, 6(10):e05242. PMCID: PMC7566100.
- Incitti T, Magli A, Jenkins J, Lin K, Yamamoto A and Perlingeiro RCR, (2020), “Pluripotent stem cell-derived skeletal muscle fibers preferentially express oxidative myosin heavy-chain isoforms: new implications for Duchenne Muscular Dystrophy”. Skeletal Muscle, 10(1):17. PMCID: PMC7268645
- Azzag K, Ortiz-Cordero C, Oliveira NAJ, Magli A, Selvaraj S, Tungtur S, Upchurch W, Iaizzo PA, Lu QL and Perlingeiro RCR, (2020) “Efficient Engraftment of Pluripotent Stem Cell-Derived Myogenic Progenitors in a Novel Immunodeficient Mouse Model of Limb Girdle Muscular Dystrophy 2I”. Skeletal Muscle, 10(1):10. PMCID: PMC7175515.
- Selvaraj S, Mondragon-Gonzalez R, Xu B, Magli A, Kim H, Lainé J, Kiley J, McKee H, Rinaldi F, Aho J, Tabti N, Shen W, & Perlingeiro RCR, (2019) “Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes”. eLIFE, 8. pii: e47970. PMCID: PMC6845233.
- Selvaraj S, Dhoke N, Kiley J, Aierdi AJM, Mondragon-Gonzalez R, Killeen G, Oliveira VKP, Tungtur S, Munain AL & Perlingeiro RCR, (2019) “Gene Correction of Limb Girdle Muscular Dystrophy Type 2A Patient-Specific iPS Cells for the Development of Targeted Autologous Cell Therapy”. Molecular Therapy,27:2147-2157. PMCID: PMC6904833.
- Selvaraj S, Kyba M & Perlingeiro RCR, (2019) “Pluripotent Stem Cell-Based Therapeutics for Muscular Dystrophies” Trends in Molecular Medicine. 25:803-816. PMCID: PMC6721995. (cover article)
- Mondragon-Gonzalez R, Azzag K, Selvaraj S, Yamamoto A & Perlingeiro RCR, (2019) “Transplantation studies reveal internuclear transfer of toxic RNA in engrafted muscles of myotonic dystrophy 1 mice”. eBioMedicine. 47:553-562. PMCID: PMC6796515.
- Magli A, Baik J, Pota P, Ortiz Cordero C, Kwak IY, Garry DJ, Love PE, Dynlacht BD and Perlingeiro RCR, (2019) “Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification”. Nature Communications, 10:2316. PMCID: PMC6534668.
Full list of publications at Experts@Minnesota or PubMed.
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Lab Members
Aaron AhlquistPosition: Researcher
Joined the lab: October 2021
Education: 2017 BS, Biology, University of Minnesota
Research Interests: Muscular dystrophy and cardiology
Contact: ahlqu043@umn.edu
Karim Azzag, Ph.D.Position: Researcher
Joined the lab: January 2018
Education: 2017 PhD, cellular biology, Montpellier II University
Research Interests: Molecular Mechanisms behind Cell Differentiation and iPSC-based cell therapy for muscular dystrophies
Accomplishments:
2022 - Abstract selected for oral presentation for the 2022 Stem Cell Institute Spring Conference: State of the Art Approaches for Cell, Tissue & Organ Transplantation.
2022 - Abstract selected for oral presentation for the 2022 29th annual FSHD society international research congress.
2022 - Abstract selected for oral presentation for the 2022 FASEB conference: The Skeletal Muscle Stem Cells and Regeneration Conference.Contact: kazzag@umn.edu
Neha Dhoke, Ph.D.Position: Researcher
Joined the Lab: December 2017
Education: PhD 2017 Stem Cell Biology and Regenerative Medicine, CSIR-Indian Institute of Chemical Technology
Research Interests: Targeting dystroglycanopathies using pluripotent stem cell-derived myogeneic progenitors
Accomplishments:
2021 - Abstract for oral presentation accepted for 2021 CardioPalooza at the University of Minnesota
2020 - Abstract for oral presentation accepted for 2020 annual meeting of the American Society for Gene and Cell TherapyContact: ndhoke@umn.edu
Jim KileyPosition: Lab Manager, Researcher
Joined the Lab: April 2013
Education: 2004 BS – Biology – University of Minnesota
Research Interests: Cardiology and Muscular Dystrophy, previous research included vascular biology and sickle cell anemia
Contact: kile0003@umn.edu
Sarah Crist, Ph.D.Position: Post-Doctoral Fellow
Joined the Lab: November 2021
Education:
2021 Ph.D. – Molecular and Cellular Biology – University of Washington/Fred Hutchinson CRC
2013 B.S. – Biology – Haverford CollegeResearch Interests: Exploring the active role of the skeletal muscle microenvironment on iPax3 myogenic progenitor maturation, with particular interest in both endogenous extracellular matrix constituents and novel deposition by the transplanted myogenic progenitors.
Accomplishments:
2022 - Harold M. Weintraub Graduate Student Award
2022 - Recipient. T32 Training Program in Cardiac Innovation, NHLBIContact: crist156@umn.edu
Phablo AbreuPosition: Post-Doctoral Associate
Joined the Lab: July 2021
Education: 2015 Ph.D. - Physiology - University of São Paulo (USP)
Research Interests: Metabolism, stem cells, and skeletal muscle regeneration
Contact: abreu030@umn.edu
Aline Miyoko Sakaguchi Yamashita, Ph.D.Position: Post-Doctoral Associate
Joined the Lab: February 2021
Education: 2019 PhD Biological Chemistry, Federal University of Rio de Janeiro
Research Interests: Molecular mechanisms during maturation of Pluripotent Stem Cell derived myogenic progenitors
Contact: sakag012@umn.edu
Bayardo Garay Position: M.D.-Ph.D. Student (MSTP)
Joined the Lab: July 2018
Education: University of California, Berkeley, B.A. Molecular and Cell Biology-Neurobiology
Research Interests: My primary scientific interest is to understand the mechanisms driving cellular degeneration with an emphasis on early detection, rescue, and prevention. One of my goals is to use patient-derived and CRISPR-Cas9-corrected iPSCs in the context of striated muscle differentiation (diseased and normal stage), and the neuromuscular junction (NMJ) as a platform for drug screening, and to unravel biological processes that might not be easily accessible in vivo. My ultimate goal is to develop an in vitro model of Duchenne and Becker muscular dystrophies using hiPSCs: skeletal muscle, motor neurons, and cardiomyocytes.
Accomplishments:
2022 - Sping and Ying Ngoh Lin Award, August 4th, 2022
2022 - Abstract selected for oral presentation for the 2022 Stem Cell Institute Spring Conference
2020 - Ruth L. Kirschstein National Research Service Award F30 Fellowship, NHLBI, March 5, 2020
2020 - Sping and Ying Ngoh Lin Award, March 7th, 2020Contact: garay034@umn.edu
Chiemelie OnyebuPosition: Undergraduate
Joined the lab: March 2022
Education: Expected Graduation Date May 2023: B.S.E., Chemical Engineering, University of Minnesota
Research Interests: Improving muscle engraftment of transplanted PSC-derived myogenic progenitors through tissue engineering.
Contact: onyeb002@umn.edu
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Lab Life