Perlingeiro Lab

Rita Perlingeiro

Our laboratory has a long-term interest in understanding the molecular mechanisms controlling lineage-specific differentiation of pluripotent stem cells (i.e. embryonic and adult reprogrammed stem cells), and applying this information to efficiently generate tissue-specific stem/progenitor cells endowed with in vivo regenerative potential. Our ultimate goal is to develop safe strategies to enable their future therapeutic application.

Rita Perlingeiro faculty profile

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Current Lab Projects
  • Dissecting the molecular mechanisms controlling in vitro and in vivo maturation of PSC-derivatives
  • Understanding how the muscle environment influences the engraftment of myogenic progenitors
  • Preclinical studies of PSC-derived myogenic progenitors to enable clinical translation
  • Disease modeling of DMD and BMD using patient-specific iPSC-derived skeletal and cardiac muscle cells
  • Understanding the impact of HLA mismatch on muscle engraftment
Selected Publications

Full list of publications at Experts@Minnesota or PubMed.

  1. Garay BI, Givens S, Stanis N, Magli A, Yücel D, Abrahante JE, Goloviznina NA, Soliman HAN, Dhoke NR, Baik J, Kyba M, van Berlo JH, Ogle B, & Perlingeiro RCR, (2022) Inhibition of mitogen-activated protein kinase pathway enhances maturation of human iPSC-derived cardiomyocytes. Stem Cell Reports. in press.
  2. Kim H, & Perlingeiro RCR, (2022) Generation of human myogenic progenitors from pluripotent stem cells for in vivo regeneration. Cellular and Molecular Life Sciences. 8;79(8):406. doi: 10.1007/s00018-022-04434-8. PMCID: PMC9270264.
  3. Ortiz-Cordero C, Bincoletto, C, Dhoke N, Selvaraj S, Magli A, Zhou H, Kim D-H, Bang AG, & Perlingeiro RCR, (2021), Defective autophagy and increased apoptosis contribute toward the pathogenesis of FKRP-associated muscular dystrophies. Stem Cell Reports. 16(11):2752-2767. PMCID: PMC8581053.
  4. Dhoke N, Kim H, Selvaraj S, Oliveira NAJ, Azzag K, Tungtur S, Ortiz-Cordero C, Kiley J, Lu QL, Bang A, & Perlingeiro RCR, (2021), A universal gene correction approach for FKRP-associated dystroglycanopathies to enable autologous cell therapyCell Reports. 36(2):109360. PMCID: PMC8327854.
  5. Ortiz-Cordero C, Magli A, Dhoke N, Kuebler T, Selvaraj S, Oliveira NA, Zhou H, Sham YY, Bang AG, & Perlingeiro RCR, (2021), “NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubes”Elife, 2021 10:e65443. PMCID: PMC7924940
  6. Ortiz-Cordero C, Azzag K, & Perlingeiro RCR, (2021), “Fukutin-Related Protein: from Pathology to Treatments”. Trends in Cell Biology, 31:197-210. PMID: 33272829 (cover article).
  7. Kim H, Selvaraj S, Kiley J, Azzag K, Garay BI, & Perlingeiro RCR, (2021), “Genomic safe harbor expression of PAX7 for the generation of engraftable myogenic progenitors”Stem Cell Reports, 16:10-19.PMCID: PMC7815936
  8. Baik J, Felices M, Yingst A, Theuer, CP, Verneris MR, Miller JS, & Perlingeiro RCR,(2020), “Therapeutic effect of TRC105 and decitabine combination in AML xenografts”. Heliyon, 6(10):e05242. PMCID: PMC7566100.
  9. Incitti T, Magli A, Jenkins J, Lin K, Yamamoto A and Perlingeiro RCR, (2020), “Pluripotent stem cell-derived skeletal muscle fibers preferentially express oxidative myosin heavy-chain isoforms: new implications for Duchenne Muscular Dystrophy”. Skeletal Muscle, 10(1):17. PMCID: PMC7268645
  10. Azzag K, Ortiz-Cordero C, Oliveira NAJ, Magli A, Selvaraj S, Tungtur S, Upchurch W, Iaizzo PA, Lu QL and Perlingeiro RCR, (2020) “Efficient Engraftment of Pluripotent Stem Cell-Derived Myogenic Progenitors in a Novel Immunodeficient Mouse Model of Limb Girdle Muscular Dystrophy 2I”Skeletal Muscle, 10(1):10. PMCID: PMC7175515.
  11. Selvaraj S, Mondragon-Gonzalez R, Xu B, Magli A, Kim H, Lainé J, Kiley J, McKee H, Rinaldi F, Aho J, Tabti N, Shen W, & Perlingeiro RCR, (2019) “Screening identifies small molecules that enhance the maturation of human pluripotent stem cell-derived myotubes”eLIFE, 8. pii: e47970. PMCID: PMC6845233.
  12. Selvaraj S, Dhoke N, Kiley J, Aierdi AJM, Mondragon-Gonzalez R, Killeen G, Oliveira VKP, Tungtur S, Munain AL & Perlingeiro RCR, (2019) “Gene Correction of Limb Girdle Muscular Dystrophy Type 2A Patient-Specific iPS Cells for the Development of Targeted Autologous Cell Therapy”Molecular Therapy,27:2147-2157. PMCID: PMC6904833.
  13. Selvaraj S, Kyba M & Perlingeiro RCR, (2019) “Pluripotent Stem Cell-Based Therapeutics for Muscular Dystrophies” Trends in Molecular Medicine. 25:803-816. PMCID: PMC6721995. (cover article)
  14. Mondragon-Gonzalez R, Azzag K, Selvaraj S, Yamamoto A & Perlingeiro RCR, (2019) “Transplantation studies reveal internuclear transfer of toxic RNA in engrafted muscles of myotonic dystrophy 1 mice”eBioMedicine. 47:553-562. PMCID: PMC6796515.
  15. Magli A, Baik J, Pota P, Ortiz Cordero C, Kwak IY, Garry DJ, Love PE, Dynlacht BD and Perlingeiro RCR, (2019) “Pax3 cooperates with Ldb1 to direct local chromosome architecture during myogenic lineage specification”. Nature Communications, 10:2316. PMCID: PMC6534668.

Full list of publications at Experts@Minnesota or PubMed.

Lab Members

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Aaron

Aaron Ahlquist

Position: Researcher

Joined the lab: October 2021

Education: 2017 BS, Biology, University of Minnesota

Research Interests: Muscular dystrophy and cardiology

Contact: ahlqu043@umn.edu


Karim

Karim Azzag, Ph.D.

Position: Researcher

Joined the lab: January 2018

Education: 2011 PhD, cellular biology, Montpellier II University

Research Interests: Molecular Mechanisms behind Cell Differentiation and iPSC-based cell therapy for muscular dystrophies

Accomplishments:
2022 - Abstract selected for oral presentation for the 2022 Stem Cell Institute Spring Conference: State of the Art Approaches for Cell, Tissue & Organ Transplantation.
2022 - Abstract selected for oral presentation for the 2022 29th annual FSHD society international research congress.
2022 - Abstract selected for oral presentation for the 2022 FASEB conference: The Skeletal Muscle Stem Cells and Regeneration Conference.

Contact: kazzag@umn.edu


Neha

Neha Dhoke, Ph.D.

Position: Researcher

Joined the Lab: December 2017

Education: PhD 2017 Stem Cell Biology and Regenerative Medicine, CSIR-Indian Institute of Chemical Technology

Research Interests: Targeting dystroglycanopathies using pluripotent stem cell-derived myogeneic progenitors

Accomplishments:
2021 - Abstract for oral presentation accepted for 2021 CardioPalooza at the University of Minnesota
2020 - Abstract for oral presentation accepted for 2020 annual meeting of the American Society for Gene and Cell Therapy

Contact: ndhoke@umn.edu


Jim

Jim Kiley

Position: Lab Manager, Researcher

Joined the Lab: April 2013

Education: 2004 BS – Biology – University of Minnesota

Research Interests: Cardiology and Muscular Dystrophy, previous research included vascular biology and sickle cell anemia

Contact: kile0003@umn.edu


Sarah

Sarah Crist, Ph.D.

Position: Post-Doctoral Fellow

Joined the Lab: November 2021

Education:
2021 Ph.D. – Molecular and Cellular Biology – University of Washington/Fred Hutchinson CRC
2013 B.S. – Biology – Haverford College

Research Interests: Exploring the active role of the skeletal muscle microenvironment on iPax3 myogenic progenitor maturation, with particular interest in both endogenous extracellular matrix constituents and novel deposition by the transplanted myogenic progenitors.

Accomplishments:
2022 - Harold M. Weintraub Graduate Student Award
2022 - Recipient. T32 Training Program in Cardiac Innovation, NHLBI
2022 - University of Washington Graduate School's 2022 Distinguished Dissertation Award (category: Biological Sciences)

Contact: crist156@umn.edu


Phablo

Phablo Abreu

Position: Post-Doctoral Associate

Joined the Lab: July 2021

Education: 2015 Ph.D. - Physiology - University of São Paulo (USP)

Research Interests: Metabolism, stem cells, and skeletal muscle regeneration

Contact: abreu030@umn.edu


Aline

Aline Miyoko Sakaguchi Yamashita, Ph.D.

Position: Post-Doctoral Associate

Joined the Lab: February 2021

Education: 2019 PhD Biological Chemistry, Federal University of Rio de Janeiro

Research Interests: Molecular mechanisms during maturation of Pluripotent Stem Cell derived myogenic progenitors

Contact: sakag012@umn.edu


Bayardo

Bayardo Garay 

Position: M.D.-Ph.D. Student (MSTP)

Joined the Lab: July 2018

Education: University of California, Berkeley, B.A. Molecular and Cell Biology-Neurobiology

Research Interests: My primary scientific interest is to understand the mechanisms driving cellular degeneration with an emphasis on early detection, rescue, and prevention. One of my goals is to use patient-derived and CRISPR-Cas9-corrected iPSCs in the context of striated muscle differentiation (diseased and normal stage), and the neuromuscular junction (NMJ) as a platform for drug screening, and to unravel biological processes that might not be easily accessible in vivo. My ultimate goal is to develop an in vitro model of Duchenne and Becker muscular dystrophies using hiPSCs: skeletal muscle, motor neurons, and cardiomyocytes.

Accomplishments: 
2022 - Sping and Ying Ngoh Lin Award, August 4th, 2022
2022 - Abstract selected for oral presentation for the 2022 Stem Cell Institute Spring Conference
2020 - Ruth L. Kirschstein National Research Service Award F30 Fellowship, NHLBI, March 5, 2020
2020 - Sping and Ying Ngoh Lin Award, March 7th, 2020

Contact: garay034@umn.edu 


Annika

Annika Bijnagte

Position: Undergraduate

Joined the lab: September 2022

Education: Expected Graduation Date May 2026: B.S., Biology, University of Minnesota

Research Interests: Muscular Dystrophy, Stem cell therapy, and Cancer cells

Contact: bijna005@umn.edu


Maggie

Maggie Huang

Position: Undergraduate

Joined the lab: September 2022

Education: Expected Graduation Date May 2025: B.S., Biochemistry, University of Minnesota

Research Interests: Cell biology, stem cells, protein expression/regulation

Contact: huan2335@umn.edu


Chiemelie

Chiemelie Onyebu

Position: Undergraduate

Joined the lab: March 2022

Education: Expected Graduation Date May 2023: B.S.E., Chemical Engineering, University of Minnesota

Research Interests: Improving muscle engraftment of transplanted PSC-derived myogenic progenitors through tissue engineering.

Contact: onyeb002@umn.edu


Kendall

Kendall Ziegler

Position: Undergraduate

Joined the lab: September 9, 2022

Education: Expected Graduation Date May 2026: B.S., Microbiology, University of Minnesota

Research Interests: Application of genome editing with CRISPR/Cas9

Contact: ziegl374@umn.edu


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