Resources & Data Transfer
The University Imaging Centers provide drive space on our Transfer Servers so that users of the facility have a method to transfer files from our equipment to other computers or offline storage devices. The purpose of this or any storage space in the UIC is not for long-term storage or the safekeeping of your data. Please remove your files from any local computer or our servers as soon as possible so that others can use the space. Per policy, files left on the UIC equipment for over 90 days will be deleted as needed. If you need longer-term storage or assistance transferring your files, please contact UIC staff to make arrangements. Thank you.
Data Transfer Servers
NAS UIC Transfer Server
An easy way to migrate files, NOT store them.
To connect to our UIC transfer server please follow the directions for your OS.
OSX (MAC): From Finder select "Go" in the menu bar then select "Connect to Server.." in the drop down menu. Enter the server address, smb://nas.uic.umn.edu/user, and click connect. Connect as a Registered User with the Name: user and Password: transfer.
Windows (PC): From Windows Explorer select "Tools" from the menu bar then select "Map network drive..." in the drop down menu. Enter the server address, \\nas.uic.umn.edu\user, check "Connect using different credentials" and click Finish. Connect to the server using the Username: user and the Password: transfer
Place all data (folders or files) within the folder named "User Data Goes Here".
Remote Access through VPN
If you are not using the University network you will need to connect via VPN to access our internal UMN transfer servers.
A VPN connection is not required to access Google Drive storage.
UMN Google Drive
With Google Drive, you can create and share files and folders online and collaborate with others on group projects. Files in Google Drive can be accessed from any smartphone, tablet, or computer.
UIC micro PET/CT Transfer Server
An easy way to move files, NOT store them.
The UIC µPET/CT Transfer Server is for µPET/CT data transfer ONLY. Any unrelated files will be removed without notification.
To connect to the µPET/CT NAS transfer server from any University networked computer.
OSX (MAC): From Finder select "Go" in the menu bar then select "Connect to Server.." in the drop down menu. Enter the server address, smb://petnas-1260f-ccrb.ahc.umn.edu, and click connect. Connect as a Registered User using your UMN login and password.
Windows (PC): From Windows Explorer select "Tools" from the menu bar then select "Map network drive..." in the drop down menu. Enter the server address, \\petnas-1260F-ccrb.ahc.umn.edu\petdata, when prompted for a user name and password, you will want to use your UMN login and password.
- If you are on a Windows machine not connected to the AD system you will need to modify your username as follows:
- ad\your UMN internet ID (your password will be your umn login password).
Electron Microscopy Transfer Server
This server is intended for transferring data from our TEM or SEM instruments. If you have acquired data using a different instrument please use a different server option.
- Username: emuser
- Password: emtransfer
- Username: emuser
- Password: emtransfer
Fiji (Fiji is just Imagej)
- A powerful free downloadable open source image processing package based on ImageJ.
Nikon NIS-Elements Viewer
- NIS Elements Viewer for PC and Mac is a free, "light" proprietary software for viewing images collected on several of our Nikon microscopes.
- Application for viewing and converting (.tif) Nikon .nd2 files.
Zeiss ZEN Lite for TIRF and Spinning Disk Confocal
- Application for viewing, enhancing and processing Zeiss .czi files.
- To install, register with Zeiss and download and install ZEN.
- A free 3D/4D microscopy image viewer for viewing raw images as well as those analysis within Imaris.
Leica Application Suite X (LAS X)
- Application to open images acquired on the Leica SP5.
- QuPath is an open, powerful, flexible, extensible software platform for whole slide image analysis.
Olympus Fluoview Viewer
- This software will read the data captured by a FV1000/FV500/FV300. Images saved with the FV file format (OIF, OIB, FV Multi-Tiff) can be read.
- This software is intended for image reading. Image capture, measurement, analysis, 3D function, etc. can not be performed using this software.
- For Windows operating systems.
HuronViewer for TissueScope LE
- Application for viewing and converting Huron TissueScope LE pyramidal tiff files.
- Compatible with Window operating systems.
Nyquist Rate & PSF Calculator
Remembering all of the steps required to set Koehler illumination can be tricky, and this app will serve as a useful reminder whenever you wish to check that the configuration of your instrument is correct.
Setting Koehler illumination generates an evenly illuminated field of view, reduces stray light, and allows you to set the balance between resolution and contrast.
Specimen Preparation Protocols
- Adhering suspended samples onto coverslips
- Coating coverslips with poly-lysine for cell culture
- Embedding of cell monolayers
- Embedding of cells in suspension
- Embedding of tissue samples
- Embedding of yeast in spurr resin
- Immunolabelling of LR white-gold sections
- Immunolabelling of ultrathin cryosections
- Preparation of cryosamples
- Immunofluorescence Mountant
- Blocking buffer for Immuno LM and EM
- Correlative LM/EM probes (Tsien Youtube video)
- Neuroglancer: WebGL-based viewer for volumetric data
- CATMAID (Collaborative Annotation Toolkit for Massive Amounts of Image Data)
- Napari: A fast, interactive, multi-dimensional image viewer for Python
- BigDataViewer: re-slicing browser for terabyte-sized multi-view image sequences
- Paintera: A general visualization tool for 3D volumetric data
- Publication: Visualizing and Interacting with Large Imaging Data
- Publication: Antibody Conjugated Oligonucleotides as a Platform for Cyclic Immunofluorescent Staining
- Publication: VIPER: A Genetically-Encoded Tag for Fluorescence and Electron Microscopy
- Publication: Simple Chemical Stains for Feature-Rish Super-Resolution and Cleared-Tissue Microscopy
External Microscopy & Imaging Links
- Invitrogen / Molecular Probes Spectra Viewer
- Invitrogen / Molecular Probes Fluorescence Tutorials
- Nikon Objective Specifications
- Nikon MicroscopyU
- What's in the picture? The temptation of image manipulation
- The JCB will let your data shine in RGB
- Interactive Focusing and Astigmatism Adventures on the Web
- Biological Buffers Reference, from Sigma-Aldrich
- Fluorescence Recovery After Photobleaching
- Biopolis Dresden Imaging Platform (BioDIP)
- Nyquist Rate and PSF Calculator
- Selected Works of George McNamara
- Nikon Learn & Explore
- The Cell: An Image Library
- Cell Painting Wiki
University of Minnesota Core Resources
- Biorepository and Laboratory Services (BLS)
- University of Minnesota Genomics Center (UMGC)
- Biotechnology Resource Center (BRC)
- University Flow Cytometry Resource (UFCR)
- Center for Mass Spectrometry and Proteomics (CMSP)
- The Characterization Facility (CharFac)
- The Minnesota Nano Center (MNC)
- Minnesota NMR Center (MNMR)