Location: 1-220H CCRB

Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. Fluorescence illumination and filters for widefield applications for DAPI, CFP, GFP, YFP, RFP, and Cy5. The scope is equipped for widefield imaging using a Hamamatsu Flash 4 V3 CMOS camera.

• A1R GaAsP Confocal System
• Laser Selection: 405nm, 445nm, 488nm, 514nm, 561nm and 640nm.
• AR1 Confocal Scanner: Nikon's exclusive hybrid scanner allows flexible switching or simultaneous use of two galvo scanners (resonant and non-resonant) this allows ultrafast imaging (up to 420fps) and photo activation imaging required to unveil cell dynamics and interaction.
• Detectors: Included are 4 confocal detectors (2 GaSaP) plus transmitted detector.

The Picoquant Fluorescence Lifetime Imaging Microscopy (FLIM) system has 405, 485 and 560 nm pulsed lasers and can perform many FLIM and FCS techniques.

Location: 1-220B CCRB

Features include Nikon’s newest motorized Ni microscope body. The newly developed CFI Plan Apochromat λ (lambda) series objectives are the key to the series’ optical performance. Nano Crystal Coat, with its ultra low refractive index, provides brighter, high-resolution and high-contrast microscopy images. The CFI 60 objectives for the Ni system are 10x, 20x, 40x oil, and 60x oil, all with DIC capabilities. An LED illumination system for fluorescence, and filters for widefield using DAPI, GFP, and Tex Red filters. The Ni stand uses Nikon’s proprietary stratum structure, used and highly acclaimed for inverted research microscopes, is now incorporated in an upright microscope, allowing a combination of components to be installed. There is a Prior motorized x/y stage and color camera for tiling of large areas of interest in brighfield, DIC, fluorescence and confocal modes.

• There are 405nm, 488nm, 561nm and 640nm laser lines for excitation.
• Dual GaSP detectors with continuous filters for spectral imaging.
• Three (3) photo-multiplier tubes (PMTs), plus transmitted light detectors.
• Color digital camera for widefield image capture, including tiling in brightfield, DIC, DAPI, AF488, AF568 and CY5 fluorescence. 
• Includes Elements modules for 2D and 3D Tracking and Measurements, Ratio and FRET imaging.

Location: 1-250D CCRB

Leica DM6000 CFS upright multiphoton microscope equipped with a TCS SP5 MP laser confocal with both galvonomic and resonance scanners and with internal spectral HyD detectors. 405, 458, 476, 488, 514, 543, 594, and 640 nm single photon laser lines and a Mai Tai eHP DS 690-1040 nm tunable multiphoton laser along with 4-channel non-descanned detectors. Objectives available include 63x/NA 1.3 oil and 25x/NA 0.95 water (optimal for intravital deep tissue imaging (up to 2.5 mm)).

Location: 1-166B Jackson Hall

Nikon A1RMP confocal is connected to an upright Nikon FN1 microscope featuring a Plan Apo LWD 25x water-immersion, NA 1.1 objective most suitable for imaging deep (it has a 2mm working distance) into a fixed or living sample. In addition to conventional and resonant visible laser confocal imaging, a Spectra Physics 15W Mai Tai eHP tunable IR laser and 4 non-descanned GaAsP detectors allow multiphoton visualization of multiple fluorophores located deep within the specimen. Visible lasers include 405, 457, 488, 514, 561 and 637 nm lines. The system has both galvonomic (4K) and resonance (720fps) scanners that can be used in concert simultaneously for photoactivation and MP imaging with enhanced hybrid descanned PMTs. An ASI piezo motorized stage(link is external) can be used for tiling or multiple-position imaging.

Location: 1-159 Jackson Hall

Nikon Ti-E Motorized Microscope featuring Nikon’s patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda coat anti-reflective coated objectives at 10x, 20x, 40x, 60x, and 100x magnifications for DIC. Fluorescence accessories for widefield applications for DAPI, GFP, RFP, and Cy5. The Nikon Elements software has the JOBS package that can automate image collection for high content screening of plates.

SIM Structured Illumination System-Using high frequency Structured Illumination, the Nikon N-SIM can achieve image resolution of 85nm, which was previously considered impossible with optical microscopes. Furthermore, with temporal resolution of up to 0.6 sec/frame, N-SIM enables super resolution time-lapse imaging capture of dynamic molecular interactions in living cells.

Location: 1-162 Jackson Hall

Olympus FluoView FV1000 is designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging, with minimal risk of damage to living specimens. The system has four fluorescence detectors plus a transmitted light detector for DIC. 

Location: 1-158 Jackson Hall

This system is equipped with pulsed 405 and 485 nm lasers for FLIM. FLIM is the acronym for "Fluorescence Lifetime Imaging Microscopy" and is the technique that maps the spatial distribution of fluorescence lifetimes within microscopic images of fixed or living cells. The fluorescence lifetime measures the time that a population of fluorescent molecules spends in the excited state. The fluorescence lifetime does not change upon intensity variations and therefore lifetime measurements are not dependent on the local concentration of fluorophores, or variations in the optical path of the microscope, the local excitation light intensity, or on the local fluorescence detection efficiency.

The FLIM is a time domain system, and uses pulsed lasers at 405 and 488 nm. The detector on the system is a Hybrid GaAsP cathode with three possible emission filter setups - a 480/30nm band pass for CFP emission, a 460LP and a 525/50 nm bandpass filter for GFP. 

Location: 1-160 Jackson Hall

Spinning disk confocal fluorescence microscope for imaging live samples at fast speed. Definite focus, time-lapse, multi-well and tiling of images. DIC and phase contrast capable. Full environmental chamber for temperature and atmosphere control. Three laser lines available (405, 488, 561 nm). 

Location: 35D Snyder Hall

The Nikon A1si is a confocal system equipped with a point-scan scan head, 5 standard PMT detectors and a 32-channel PMT spectral detector. The A1si's highest resolution mode is up to 4096 x 4096 pixels. The system is mounted on a Nikon Eclipse Ti2 inverted, fluorescence microscope with DIC optics. NIS Elements imaging software is used to control acquisition and analysis.

Location: 23B Snyder Hall

The AZ100 Multizoom Macroscope with the C1si Spectral Confocal attachment, make the ultimate platform for in vivo imaging. Ideal for plant biology, developmental biology, cell biology, stem cell and tissue research, the AZ-C1si allows the researcher to view large specimens in confocal mode. The AZ-C1si can capture fields of view up to 1cm and permits deeper confocal imaging than conventional microscopes thanks to its large working distance objectives. Whole organisms can be monitored and documented over time (for example, embryos) offering a wealth of continuous information on development or the organism’s response to experimental variables. Both conventional PMTs and Spectral detectors are installed on this instrument.