Confocal Microscopes

Requesting Training and Services

If you are interested in gaining access to UIC resources or requesting services please follow the steps on our training and service request page.

Cancer and Cardiovascular Research Building (CCRB)

Expand all

Cancer and Cardiovascular Research Building (CCRB)

Caliber I.D. RS-G4 Ribbon Scanning Confocal

uic_rsg4

The RS-G4 has been designed with a compact, high-speed resonance scanner and scanning stage with macro and micro-imaging optics that allow the acquisition of in-vivo and ex-vivo images from slides to cleared whole tissue mounts in the micrometer to centimeter scales.

In addition, ex-vivo tissue sections can be imaged in both reflectance and fluorescence modes. Its 360-degree rotation of the scan head allows greater flexibility and precise angling for acquiring images of large tissue, organs, or whole animals. With a single nose piece, the instrument is essentially agnostic as to what type of objectives can be used. We routinely use our Nikon Plan Apo 10xC/0.5 NA (WD: 5.5 mm) and the Nikon CFI90 20xC/1.0 NA (WD: 8.2 mm) glycerol immersion objectives for imaging cleared tissue.

References

Equipment Specifications

We have objective options from 1x to 100x, including the Nikon CFI90 20xC/1.0 NA (WD: 8.2 mm) objective designed for cleared samples. Resolution is determined by which objective is used with individual frames (1024 x 1024 pixels) captured at up to 25fps.

Highlights

  • Supports air, glycerol, water, and oil immersion objectives
  • Fast, large-area mosaic creation up to 120 x 80 mm x 11mm
  • Simultaneous and sequential imaging for reflectance and fluorescence
  • Experiment acquisition setup for snapshots, mosaics, time, Z-series, and multi-points. wavelength

Lasers

  • Wavelengths include: 405 nm, 488 nm, 561 nm, 638 nm, 785 nm

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

2x

0.10

PlanApo λ

 

 

8.5

Air

4x

0.20

PlanApo

 

∞/-

15.7

Air

10x

0.50

PlanApo

Correction Collar

∞/-

5.5

Glycerol+

10x

0.45

PlanApo

DIC N1

∞/0.17

4

Air

20x

1.00

 

Correction Collar

∞/0

8.2

Glycerol+

ELWD 20x

0.45

S Plan Fluor

Correction Collar/ Ph1 Adm

∞/0-2

8.2-6.9

Air

Leica SP5 Upright Confocal Microscope

uic_leica_sp5

Leica DM6000 CFS upright multiphoton microscope equipped with a TCS SP5 MP laser confocal with both galvonomic and resonance scanners and with internal spectral HyD detectors. 405, 458, 476, 488, 514, 543, 594, and 640 nm single photon laser lines and a Mai Tai eHP DS 690-1040 nm tunable multiphoton laser along with 4-channel non-descanned detectors. Objectives available include 63x/NA 1.4 oil and 25x/NA 0.95 water (optimal for intravital deep tissue imaging (up to 2.5 mm)).

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

5x

0.15

HCX PL FLUOTAR/CS

 

∞/-/C

13.7

Air

10x

0.4

HC PL APO/CS

Confocal Series

∞/0.17/A

2.2

Air

20x

0.7

IMM CORR/CS

Confocal Series

∞/-/C

0.26

Water-Glycerol-Oil

L 25x

0.95

HCX IR APO/CS

VISIR

∞/0.17/0

2.4

Water Immersion

L 25x

0.95

HC FLUOTAR

VISIR

∞/0/0

2.5

Water Dipping

40x

1.3

HCX PL APO/CS

Confocal Series

∞/0.17/0

0.24

Oil

63x

1.4-0.60

HCX PL APO/CS

Confocal Series

∞/0.17/E

0.14

Oil

Nikon A1R FLIM Confocal Microscope

uic_a1rflim

Features include Nikon's patented PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and reflected light applications. New Lambda anti-reflective coated objectives at 4x, 10x, 20x, 20x water immersion, 40x oil, and 60x oil magnifications for DIC. Fluorescence illumination and filters for widefield applications for DAPI, CFP, GFP, YFP, RFP, and Cy5. The scope is equipped for widefield imaging using a Hamamatsu Flash 4 V3 CMOS camera. Automated collection and analysis with Nikon Elements JOBS is available.

 

  • A1R GaAsP Confocal System
  • Laser Selection: 405 nm, 445 nm, 488 nm, 514 nm, 561 nm, and 640 nm.
  • The Picoquant Fluorescence Lifetime Imaging Microscopy (FLIM) system has 405 nm, 485 nm, and 560 nm pulsed lasers and can perform many FLIM and FCS techniques.
  • A1R Confocal Scanner: Nikon's exclusive hybrid scanner allows flexible switching of two galvo scanners (resonant and non-resonant), this allows ultrafast imaging (up to 420fps) and photoactivation imaging required to unveil cell dynamics and interaction.
  • Detectors: Two GaSaP detectors, two enhanced PMT detectors, one transmitted light detector, one Hamamatsu Flash 4.0 V3 CMOS camera
  • Configured for automated screening of plates and slides
  • A modular system for assay and analysis development (Nikon Elements and JOBS)
  • Environmental control for live cell imaging

References

Objectives

* Some objectives have a correction collar for various coverslip thicknesses. See staff for appropriate adjustments.

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

4x

0.20

PlanApo λ

 

∞/-

20

Air

10x

0.45

PlanApo λ

 

∞/0.17

4.0

Air

20X

0.75

PlanApo λ

DIC N2

∞/0.17

1.0

Air

20x*

0.95

Apo LWD

LWD

∞/0.11-0.23

0.8

Water

40x

1.30

PlanFluor

DIC N2

∞/0.17

0.24

Oil

40x

0.95

Plan Apo 

DIC M/N2

∞/0.11-0.23

0.14

Air

40x

0.60

SPlanFluor

ELWD, NAMC3

∞/0-2

3.6-2.8

Air

60x

1.40

Plan Apo λ

DIC N2

∞/0.17

0.13

Oil

60x*

1.2

Plan Apo VC 

 

∞/0.15-0.18

0.27

Water

Nikon C2+ Upright Spectral Confocal and Widefield Imaging System

uic_c2

Features include Nikon’s motorized Ni microscope body. The CFI60 objectives for the Ni system are 10x, 20x, 40x oil, and 60x oil, all with DIC capabilities. An LED illumination system for fluorescence, and filters for widefield using DAPI, GFP, and Tex Red filters. There is a Prior motorized x/y stage and color camera for tiling of large areas of interest in brighfield, DIC, fluorescence, and confocal modes.

  • There are 405 nm, 488 nm, 561 nm, and 640 nm laser lines for excitation
  • Dual GaSP detectors with continuous filters for spectral imaging

 

  • Three photo-multiplier tubes (PMTs), plus transmitted light detectors, Nikon DS-U3 color camera
  • Transmitted Light Filters: ND2, ND4, ND8, ND16, Green, and Cool Blue
  • Color digital camera for widefield image capture, including tiling in brightfield, DIC, DAPI, AF488, AF568 and CY5 fluorescence
  • Includes Elements modules for 2D and 3D Tracking and Measurements, Ratio and FRET imaging

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

4x

0.13

PlanFluor

PhL DL

∞/1.2

16.5

Air

10x

0.45

PlanApo λ 

DIC N1

∞/0.17

4.0

Air

20x

0.75

PlanApo λ 

DIC N2

∞/0.17

1.0

Air

40x

1.30

PlanFluor

DIC N2

∞/0.17

0.24

Oil

60x

1.40

PlanApo

DIC N2

∞/0.17

0.14

Oil

100x

1.40

PlanApo VC

DIC N2

∞/0.17

0.13

Oil

Jackson Hall

Expand all

Jackson Hall

Nikon A1RHD Multiphoton Microscope

uic_nikon_a1rhdmp

Nikon A1RMP confocal is connected to an upright Nikon FN1 microscope featuring a Plan Apo LWD 25x water immersion, NA 1.1 objective most suitable for imaging deep (it has a 2 mm working distance) into a fixed or living sample. In addition to laser scanning confocal imaging, conventional, and resonant visible laser confocal imaging, a Spectra-Physics 15W Mai Tai eHP tunable IR laser and six, three GaAsPs (epi direction) and three conventional PMTs (trans direction) non-descanned GaAsP detectors allow multiphoton visualization of multiple fluorophores located deep within the specimen. Visible lasers include 405, 457, 488, 514, 561 and 637 nm lines. The system has both galvonomic (4K) and resonant (1k, 8Khz, up to 720fps) scanners that can be used in concert simultaneously for photoactivation and MP imaging. A encoded Prior motorized stage and 200 µm Piezo objective motor can be used for tiling or multiple-position imaging.

The system is also equipped with Second Harmonic Generation (SHG) detection.  SHG doesn't involve the excitation of molecules like other techniques such as fluorescence microscopy. See UIC staff for training and questions.

This instrument was funded in 2012 by the Minnesota Partnership in conjunction with the Mayo Clinic Foundation, Rochester, MN.

In October 2018 the system was upgraded to an A1RHD with 1024 resonant scanning and improved signal-to-noise imaging in resonant mode.

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion

10x

0.3

PlanFluor

DIC L

∞/0.17

16

Dry

10x

0.3

Fluor

DIC L

∞/0

2

Water

10x

0.5

Plan Apo

Correction Collar

∞/-

5.5

Glycerol

20x

0.45

PlanFluor

DIC L

∞/0-2

7.4

Dry

20x

0.75

PlanApo lambda

DIC N2

∞/0.17

1

Dry

25x

1.1

ApoLWD

DIC 2, Correction Collar

∞/0-0.17

2

Water

40x

0.8

Fluor

DIC M

∞/0

2

Water

100x

1.3

PlanFluor

 

∞/0.17

0.2

Oil

 

Filters - MP

Filter cube Emission band Common dyes
99020 450/40 DAPI
9920-IR 460/50 DAPI
99022 525/50 GFP, AF488
99021 485/30 CFP
99023 600/50 mCherry, AF568
99023 660LP AF647, Cy5
99024 545/40 YFP

 

Filters - MP NDD

Filter cube Emission band Common dyes
Epi NDDs
99124 425/50

SHG collagen, DAPI TPE

99121 510/80 GFP
99121 605/70 mCherry
Trasn NDDs
99020 450/40

SHG collagen, DAPI TPE

99121 515/70 GFP
99121 605/70 mCherry
Available on demand
99321 440/80

SHG collagen, DAPI TPE

99324 540/80 YFP
99324 620/52 mCherry

Nikon A1Rsi HD Confocal with SIM Super Resolution

uic_a1rsi_sim

Nikon Ti-E Motorized Microscope featuring PFS Perfect Focus III focus lock technology. LED Illumination systems for both transmitted and fluorescence light applications. Objectives at 10x, 20x, 40x, 60x (water and oil immersion), and 100x magnifications for DIC. Fluorescence accessories for widefield applications for DAPI, GFP, RFP, and Cy5. The Nikon Elements software has the JOBS package that can automate image collection for high-content screening of plates.

SIM Structured Illumination System-Using high-frequency Structured Illumination, the Nikon N-SIM can achieve an image resolution of 120 nm, which was previously considered impossible with optical microscopes. Furthermore, with a temporal resolution of up to 0.6 sec/frame, N-SIM enables super-resolution time-lapse imaging capture of dynamic molecular interactions in living cells.

  • Lasers (A1R): 405 nm, 488 nm, 561 nm, 640 nm
  • Lasers (N-SIM): 405 nm, 488, nm, 561 nm, 640 nm
  • Detectors: The A1R has four PMTs for confocal detection, two of them GaAsPs, with double the sensitivity of traditional PMTs. There is also a transmitted light detector and a 32-channel spectral detector.
  • N-SIM: Hamamatsu FLASH 4

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion

2x

0.1

PlanApo

   

8.5 mm

Dry

10x

0.45

PlanApo

DIC N1

∞/0.17

4

Dry

20x

0.75

PlanApo VC

DIC N2

∞/0.17

1

Dry

40x

0.6

S PlanFluor

DIC N1, Correction Collar

∞/0-2

3.6

Dry

40x

0.95

PlanApo

DIC MN 2, Correction collar

∞/0.11-0.23

0.14

Dry

60x

1.27

PlanApo IR with correction collar

DIC N2

∞/0.15-0.19

0.18-0.16

Water

60x

1.4

Plan Apo Lambda

DIC N2

∞/0.17

0.13

Oil

100x

1.49

SR ApoTIRF with correction collar

DIC N2

∞/0.13

0.12

Oil

 

Filters - Confocal

Filter cube Emission band Common dyes
99020 450/40 DAPI
99022 525/50 GFP, AF488
99023 600/50 mCherry, AF568
99023 660LP AF647, Cy5

 

Filters - Camera

Filter cube Excitation band Dichromatic mirror Emission band Common dyes
Installed
Chroma 49028 395/25 T425lprx 460/50 DAPI
Chroma 49002 470/40 T495lprx 525/50 GFP, AF488
Chroma 49005 545/30 T570lp 620/60 dsRed, AF568
Chroma 49006 620/60 T660lprx 700/75 AF647, Cy5
Available on demand
Chroma 49303 495/25 T515lprx 537/29 Opal 520
Chroma 49304 546/10 T556lprx 572/23 Opal 570
Chroma 49306 580/25 T600lprx 615/30 Opal 620
Opal 620 590/20   635/75 Opal 620
Chroma 49022 650/45 T685lprx 720/60 Opal 690, Cy5.5

 

Filters - SIM

Filter cube Excitation band Dichromatic mirror Emission band Effective emission window Common dyes
Installed
SIM405 395/20 440LP 460/50 440-485 DAPI
SIM488 480/20 515LP 523/45 515-545 GFP, AF488
SIM561 561/10 590LP 605/70 590-640 mCherry, AF568
SIM640 620/60 665LP 700/75 665-738 AF647, Cy5

Olympus FLUOVIEW FV1000 BX2 Upright Confocal

uic_bx2

Olympus FluoView FV1000 is designed for high-resolution, confocal observation of both fixed and living cells. The FV1000 offers advances in confocal system performance while providing the speed and sensitivity required for live cell imaging, with minimal risk of damage to living specimens. The system has four fluorescence detectors plus a transmitted light detector for DIC. 

 

  • Automated upright  BX61 microscope base
  • 100W Mercury fluorescent lamp
  • 100W halogen transmitted illuminator with detector
  • PRIOR ProScanII motorized XY stage 
  • DIC optics

​Methods

  • Fluorescence, Brightfield, DIC, FRAP, FRET

​Automated Capabilities

  • Time-lapse, Multi-channel fluorescence, Co-localization, Z-stacks 

Lasers

  • 405nm 25mW Diode, 458, 488, 515nm 30mW Argon, 543nm 1mW HeNe green, 635nm 10mW HeNe red

Detector 

  • 4 photomultiplier tubes channels, 1 transmission detector

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion

4x

0.16

UPlanApo

--

∞/0.17

13

Dry

10x

0.4

UPlanApo

BX2-DIC10

∞/0.17

3.1

Dry

20x

0.85

UPlanSApo

BX2-DIC20

∞/-/FN26.5

0.17

Oil

40x

1.3

UPlanFLN

BX2-DIC40

∞/0.17/FN26.5

0.2

Oil

60x

1.42

UPlanApo N

BX2-DIC60

∞/0.17/FN26.5

0.15

Oil

60x

0.9

LUMPlanFL

 

∞/0

2

Water

 

Filters

Filter

Excitation Filter (nm)

Dichroic Mirror (nm)

Emission Filter (nm)

DAPI

D350/50

BS20/80

460/50

FITC

D480/30

DM405/488

D535/40

TRITC

D540/25

DM405/488/543/635

D605/55

Cy5

D620/20

DM458/515

D700/75

 

Channels

Channels

Dichroic Mirror (nm)

Barrier Filters (nm)

Channel 1

SDM560, SDM510, SDM490

BA505-525, BA480-495, BA430-470

Channel 2

SDM560

BA505-605, BA560-620, BA535-565, BA505-525

Channel 3

SDM640

BA560-660

Channel 4

--

BA655-755

Olympus FLUOVIEW FV1000 IX2 Inverted Confocal with FLIM Detector

uic_ix2

This system is equipped with pulsed 405 and 485 nm lasers for FLIM. FLIM is the acronym for "Fluorescence Lifetime Imaging Microscopy" and is the technique that maps the spatial distribution of fluorescence lifetimes within microscopic images of fixed or living cells. The fluorescence lifetime measures the time that a population of fluorescent molecules spends in the excited state. The fluorescence lifetime does not change upon intensity variations and therefore lifetime measurements are not dependent on the local concentration of fluorophores, or variations in the optical path of the microscope, the local excitation light intensity, or on the local fluorescence detection efficiency.

The FLIM is a time domain system and uses pulsed lasers at 405 and 488 nm. The detector on the system is a Hybrid GaAsP cathode with three possible emission filter setups - a 480/30nm bandpass for CFP emission, a 460LP, and a 525/50 nm bandpass filter for GFP. 

Automated Capabilities

  • Time-lapse, Stitching, Multi-Channel fluorescence, Co-localization, Spectral unmixing, Z-stacks

External FLIM

  • PicoQuant pulsed lasers at 405 and 485 nm
  • Software includes Becker&Hickl SPCM and SPC Image

Lasers 

  • 405nm 25mW Diode
  • 458, 488, 515nm 30mW Argon
  • 543nm 1mW HeNe green
  • 635nm 10mW HeNe red

Detector

  • 3 photomultiplier tubes channels
  • 1 transmission detector

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion

4x

0.16

UPlanSApo

--

∞/-/FN26.5

13

Dry

10x

0.4

UPlanSApo

IX2-DIC10

∞/0.17/FN26.5

3.1

Dry

20x

0.85

UPlanSApo

IX2-DIC20

∞/-/FN26.5

0.17

Oil

40x

1.3

UPlanFLN

IX2-DIC40

∞/0.17/FN26.5

0.2

Oil

60x

1.42

UPlanApo N

IX2-DIC60

∞/0.17/FN26.5

0.15

Oil

 

Filters

Filter

Excitation Filter (nm)

Dichroic Mirror (nm)

Emission Filter (nm)

DAPI

D350/50

BS20/80

460/50

FITC

D480/30

DM405/488

D535/40

TRITC

D540/25

405/488/543

D605/55

Cy5

D640/20

DM458/515

D680/30

 

Channels

Channels

Dichroic Mirror (nm)

Barrier Filters (nm)

Channel 1

SDM560, SDM510, SDM490

2nm Spectral Monchrometer Grating

Channel 2

SDM640, SDM560

2nm Spectral Monchrometer Grating

Channel 3

SDM640

BA560-620, BA560-660, BA655-755

Zeiss Cell Observer Spinning Disk Confocal

uic_zeiss_sd

Zeiss Cell Observer Spinning Disk Confocal

The Zeiss Cell Observer Spinning Disk confocal fluorescence microscope for imaging live samples at fast speed. Definite focus, time-lapse, multi-well, and tiling of images. DIC and phase contrast capable. Full environmental chamber for temperature and atmosphere control. Three laser lines are available (405, 488, and 561 nm). 

Motorized Zeiss Axio Observer Z1 inverted scope with temperature-controlled enclosure, CO2, Definite Focus, multi-channel acquisition, piezo x/y/z stage.

Methods

  • Fluorescence, Brightfield, Phase contrast, DIC, Time Lapse, Live Cell with 37°C / CO2 enclosure

Automated Capabilities

  • Time-lapse, Stitching, Multi-channel fluorescence, Co-localization, Piezo Z-stacks

Lasers

  • 405 nm, 50 mW, 488 nm, 100 mW, 561 nm, 30 mW

Detector

  • Photometrics QuantEM 512SC - High Sensitivity 16 bit, 512 x 512 grayscale CCD

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

10x

0.3

EC Plan-NEOFLUAR

Ph1

∞/-

3.2

Air

20x

0.75

Plan-ApoCHROMAT

Phase 2, DIC II

∞/0.17

0.61

Air

40x

0.95

EC Plan-APOCHROMAT

KORR

∞/0.13-0.21

 

Air

             

63x

1.2

C-ApoCHROMAT

KORR UV-VIS-IR, Correction Collar

∞/0.17

0.28

Water Immersion

100x

1.4

Plan-ApoCHROMAT

DIC

∞/0.17

0.11

Oil Immersion

Snyder Hall

Expand all

Snyder Hall

Nikon AX R

uic_ax_r
  • Nikon NiE fully motorized (multi-position nosepiece, filters, condenser, z-motor, x/y stage) upright microscope with 25 mm field of view. This resonant (15 fps full FOV with up to 720 fps at 2048x16 pixels) and galvanomic scanners (up to 8192x8192 pixels for Nyquist resolution at lower magnifications or larger FOVs)
  • Lasers and detection filters utilizing the  408, 445, 488, 514, 561, and 640  nm excitation lines allow for multiplexed imaging of DAPI, CFP, YFP, GFP, FITC, dsRed, TRITC, CY5, and many more fluorescent reporters.
  • Four-channel GaAsP PMT detector modules, including spectral detection along with transmitted light.

 

  • Objectives 2x, 4x, 10x, 20x, 40x, 60x, 100x including water, silicon and oil immersion options. The system is compatible with many of the UIC’s extensive collection of additional Nikon objectives.
  • Differential Interference Contrast (DIC)
  • Nikon Element ER software has built-in multidimensional (multi-XY, Z, T, multichannel) experiment capabilities. The JOBS module allows customization in setting up non-orthogonal experiments with multiple paths and dimensions. Analysis of data can be done in real-time during the experiment, and the direction of the experiment can even be changed based on the results of the analysis. Fully automated collection and analysis capabilities with JOBS. The UIC staff is excited to help train you from the most basic image acquisition to the full complement of our instrument's capabilities.

Nikon A1si Confocal and Widefield Microscope

uic_a1si

The Nikon A1si is a confocal system equipped with a point-scan confocal scanner, four PMT detectors for fluorescence and one for transmitted light. The scope also has a 32-channel PMT spectral detector. The A1si's highest pixel resolution mode is up to 4096 x 4096 pixels. The system is mounted on a Nikon Eclipse Ti2 inverted scope equipped with Perfect Focus System, fluorescence filters with DIC optics and a widefield Hamamatsu Flash 4 camera. There is also an IBIDI environmental stage top incubator system for long-term live cell imaging. Nikon NIS Elements imaging software is used to control acquisition and analysis. Nikon JOBS will permit investigators to create complex automated image acquisitions with intelligent analysis.

The advantages of multispectral imaging and unmixing have been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging.

Lasers 

  • 405 nm diode at 38mW, Argon multiline laser 457/477/488/514nm at 40mW, 561 nm diode at 40mW, 638 nm diode at 10mW

Detectors

  • 4 PMTs, a Transmitted light detector, and a 32-channel spectral detector

References

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging" Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct; Chapter 14:Unit 14.19. DOI: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

4x

0.2

Plan Apo

--

∞/--

20

Air

10x

0.3

Plan Apo

--

∞/0.17

4

Air

20x

0.75

Plan Apo

DIC N2

∞/0.17

1

Air

60x

1.2

Plan Apo

DIC N2, Correction collar

∞/0.15-0.18

0.27

Water

60x

1.4

Plan Apo

DIC H

∞/0.17

0.21

Oil

 

Filters

Widefield Filters

Common Fluorophore

Excitation Filter (nm) Dichroic Mirror (nm) Emission Filter (nm)

DAPI

340-380 400LP 435-485

CFP

426-446 455LP 460-500

GFP

465-495 505LP 515-555

YFP

490-515 520LP 520-560
Texas Red 532-587 595LP 608-683

 

Confocal Filters

Common Fluorophore

Excitation Filter (nm) Dichroic Mirror (nm) Emission Filter (nm)

DAPI

DS350/50 BS20/80 460/50

CFP

DS440/40 400-457/514 470/40

GFP

D480/30 DM405/488 D535/40

YFP

DS 510/50 400-457/514 D 555/30
dsRed DS540/25 408/488/543 D600/50
Cy5 DS640/20 DM458/515 D685/70

Nikon AZ100 C1si Spectral Confocal Microscope

uic_az100_c1si

The AZ100 Multizoom Macroscope with the C1si Spectral Confocal attachment makes the ultimate platform for in vivo imaging. Ideal for plant biology, developmental biology, cell biology, stem cell, and tissue research, the AZ-C1si allows the researcher to view large specimens in confocal mode. The AZ-C1si can capture fields of view up to 1cm and permits deeper confocal imaging than conventional microscopes thanks to its large working distance objectives. Whole organisms can be monitored and documented over time (for example, embryos) offering a wealth of continuous information on development or the organism’s response to experimental variables. Both conventional PMTs and Spectral detectors are installed on this instrument.

The system is equipped with 1x, 4x, and 5x objectives with variable optical zoom.  There are 405, 457, 476, 488, 514, 561, and 637 nm lasers for image, co-localization, FRAP, FRET, and spectral studies.

The system is run using Nikon Elements Software.

The advantages of multispectral imaging and unmixing have been shown in hundreds of publications to reduce autofluorescence by as much as 100-fold, both for microscopy and FFPE tissues and in small animal and plant fluorescence imaging. 

Lasers

405 nm, 457 nm, 488 nm, 514 nm, 567 nm, 637 nm

References

Tam, et al "Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging" Molecular Imaging, 6, 2007, 269.

Mansfield, et al "Visualization of microscopy-based spectral imaging data from multi-label tissue sections" Current Protocols in Molecular Biology, 2008 Oct; Chapter 14:Unit 14.19. DOI: 10.1002/0471142727.mb1419s84.

Levenson, et al "Multispectral imaging in biology and medicine: slices of life." Cytometry A. 2006 Aug 1;69(8):748-58.

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

1x

0.1

AZ Plan Apo

 

 

35 mm

Air

4x

0.4

AZ Plan Apo

 

 

20 mm

Air

5x

0.5

AZ Plan Fluor

 

 

15 mm

Air

 

Filters

Filter

Wavelengths

Excitation Filter (nm)

Dichoric Mirror (nm)

Emission Filter (nm)

DAPI

D350/50

BS20/80

460/50

GFP

D480/30

DM405/488

D535/40

TRITC

D540/25

405/488/543

D605/55

Cy5

D640/20

DM458/515

D680/30

 

Channels

Channels

Dichoric Mirror (nm)

Barrier Filters (nm)

Channel 1

SDM560, SDM510, SDM490

2nm Spectral Monchrometer Grating

Channel 2

SDM640, SDM560

2nm Spectral Monchrometer Grating

Channel 3

SDM640

BA560-620, BA560-660, BA655-755

Wallin Medical Biosciences Building (WMBB)

Expand all

Wallin Medical Biosciences Building (WMBB)

Leica Stellaris 8

uic_stellaris_8

Leica DMi8 inverted laser scanning confocal microscope with both galvonomic and resonance scanners and 5 spectral internal HyD-S detectors.  A fixed 405nm violet laser and pulsed white-light laser (WLL) that is tunable from 440-790nm allows for precise excitation control and multi-spectral imaging.  The WLL and HyD-S detectors allow for Leica's patented Tau gating and separation modalities using fluorescence lifetime measurements.  Environmental control enables long-term live cell imaging and the Leica Navigator interface streamlines stage control and multi-position high-speed tiling. 

 

Objectives include 20x/0.7NA multi-immersion, 40x 1.25NA glycerol motCORR, 63x 1.4NA oil, and 63x 1.2NA water.