University Imaging Center Services
Overview
In addition to training users to independently operate our instrumentation, the University Imaging Centers offers several end-to-end research and full sample preparation services to University of Minnesota investigators as well as external users.
Full Service Sample Preparation
We have the expertise, equipment, and reagents to prepare specimens for some of your light and electron microscopy needs including tissue clearing, immunofluorescence and immunogold methods. Multiplexed Ion Beam Imaging (MIBI) sample preparation services are also now available.
Free Secondary Antibodies
The University Imaging Centers offer free Thermo Fisher Scientific secondary antibodies (20 µl aliquots) at all UIC locations.
Contact UIC staff to get yours! All the antibodies are derived in goat and available in anti-mouse, anti-rabbit and anti-rat versions, directed to the whole IgG molecule and are highly cross-absorbed. Emission selections are optimized for UIC scopes, so they are intened for UIC users.
List of Available Antibody Aliquot's
For more details, visit the Thermo Fisher Antibodies(link is external) webpage.
Antibody Aliquot Preparation Protocol
| Specificity | Reference | Dye |
| Mouse | Alexa 405 | |
| Alexa 488 | ||
| Alexa 568 | ||
| Alexa 647 | ||
| Rat | Alexa 405 | |
| Alexa 488 | ||
| Alexa 568 | ||
| Alexa 647 | ||
| Rabbit | Alexa 405 | |
| Alexa 488 | ||
| Alexa 568 | ||
| Alexa 647 |
EM Sample Preparation
The University Imaging Centers are fully equipped for SEM, TEM, and Serial Block-Face SEM sample preparation.
SPATIALOMICS
The University Imaging Centers and University of Minnesota Genomics Center are excited to announce the arrival of Spatial Transcriptomics at the University of Minnesota.
Training
The University Imaging Centers (UIC) is a fee-for-use facility open to investigators and projects of members of the University of Minnesota community as well as external users.
MIBI
The UIC Multiplexed Ion Beam Imaging (MIBI) System is a revolutionary imaging platform, enabling comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment.
