In response to the COVID-19 pandemic, the University Imaging Centers are continuing to provide status updates, and have implemented mandatory cleaning and safety protocols for all users using UIC resources. Please visit our sunrise safety protocols page to learn more 

Light Microscopes

Cancer & Cardiovascular Research Building (CCRB)

Nikon Inverted Ti-E Deconvolution Microscope

Nikon TiE

Location: 1-220G CCRB

The Nikon Ti-E Motorized Microscope with Ibidi live cell environment chamber features include Nikon’s patented PFS Perfect Focus III focus lock technology. 100 Watt Illumination system for transmitted and LED reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x*, 40x* NAMC Contrast, 60x, and 100x DIC magnifications (*correction collars). Fluorescence accessories for widefield applications for DAPI, GFP, CFP, YFP, RFP and Cy5...and many others. Note: the 20x and 40x objective have correction collars that are adjusted for each user's plate or coverslip thickness.

Elements software and an offline computer just outside the scope room in CCRB are available. The workstation includes Elements Deconvolution LIM2D/3D module,  a customizable user interface to run deconvolution on entire batches of files.

Learn more about the Nikon Inverted Ti-E Deconvolution Microscope

The 20x and 40x objectives on this scope can be adjusted for use with various thickness sample plates and slides.  

Details on how to adjust these objectives with correction collars can be found here: http://www.microscopyu.com/tutorials/java/aberrations/correctioncollar/i...(link is external)

Fluorescence Illumination: A Lumencor SpectraX pulsed light engine provides excitation with minimize photobleaching/phototoxicity.

Learn more about how deconvolution can improve optical image data: https://www.youtube.com/watch?v=MJ2FOFqr7hw(link is external)

Live Cell Reagents:

Learn more about live cell imaging reagents here:https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellu...(link is external)

Ibidi Environmental Stage with CO2 and O2 regulation:

https://ibidi.com/stage-top-incubators/231-ibidi-stage-top-incubation-system-universal-fit-for-1-chamber-co2o2.html

Dishes and chambered slides can be found here:

https://ibidi.com/12-labware(link is external)

We also have the Braintree Scientific Automated Syringe pump to perfuse solutions! Holds 6 syringes up to 50cc.

http://www.braintreesci.com/prodinfo.asp?number=BS-9000

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
4x 0.13 PlanFluor -- ∞/-- 17.2 Air
10x 0.45 PlanApo DIC N1 ∞/0.17 4 Air
20x 0.75 PlanApo DIC MN2 ∞/0.17 1 Air
40x 0.95 Plan Apo DIC MN2, Correction Collar ∞/0.11-0.23 0.14 Air
60x 1.4 PlanApo DIC N2 ∞/0.17 0.13 Oil Immersion
100x 1.45 PlanApo DIC N2 ∞/0.17 0.13 Oil Immersion

Leica LMD6500 Laser Dissection Microscope

lmd6500

Location: 1-220B CCRB

Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected for subsequent molecular analysis.

Researchers can selectively and routinely analyze regions of interest down to single cells from all kinds of tissues (cryo or paraffin sections 4-20 µm thick), even living cells from cell culture. 

You have the ability to de-paraffinize your slides on site.

Learn more about the Leica LMD6500 Laser Dissection Microscope

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
1.25 0.04 HCX FL FLUOTAR   ∞/-/^ 3.7  
5x 0.12 UVI   ∞/- 11.7 Air
10x 0.3 HCX FL FLUOTAR Ph2 ∞/-/D 11 Air
20x 0.4 HCX FL FLUOTAR Ph1 ∞/0-2/C 6.9 Air
40x 0.6 HCX FL FLUOTAR Corr XT ∞/0-2/C 3.3-1.9 Air
63x 0.7 HCX FL FLUOTAR Ph2 ∞/0.1-1.3/C 2.6 Air

Filters:

Filter Wavelengths Filter Wheel Position
Excitation Filter (nm) Dichoric Mirror (nm) Emission Filter (nm)
DAPI 360-370 (365 CWL) 380 LP 420 LP Open
U/B/G (triple DAPI/GFP/dsRed) 385-415 (405 CWL) 410-470 (440 CWL) 450-470 (460 CWL) UV/B/G
485-505 (495 CWL) 505-55 (530 CWL) 510-540 (525 CWL)
555-585 (570 CWL) 575 LP 590-650 (620 CWL)

Transmitted Light Filters:

  • ND2, ND4, ND8, and ND16
  • Green and cool blue

Lasers:

  • Wavelength: 355 nm
  • Max. pulse energy: 70 µJ
  • Pulse frequency: 80 Hz

Detector:

  • LMD CC7000 color camera

Leica MZ FL III Fluorescence Stereomicroscope

Leica MZ lll

Location: 1-250C CCRB

Sort, manipulate and image your specimens with the stereo objective in 3D, with large fields of view, large working distances and an outstanding depth of field. Simply switch to the HR (High Resolution) micro-objective and analyze the quality of the gene expression immediately and without delay via an optical light microscope at a resolution of 0.7 µm (1320 pairs of lines/mm) and an 800x magnification.

Learn more about the Leica MZ III Fluorescence Stereomicroscope

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm)
0.5x   Plan   139 Air
1x 0.035 PlanApo -/- 55 Air
2x 0.07 PlanApo -/- 15 Air

Filters:

Filter  Wavelengths 
 Excitation Filter (nm)  Emission Filter (nm)
 UV  D350/50x  E420 1pv2
GFP1   ET425/60x  ET505/40m
GFP2   480/40  510
 546/10  590
Detector: 

Leica DFC420 C camera.

Digital 5 MP color camera with peltier cooling system to capture high-resolution images for analysis, documentation and presentation.

http://www.leica-microsystems.com/products/microscope-cameras/industry/d...

Jackson Hall

Zeiss TIRF Scope

ZeissTIRF

Location: 1-164 Jackson Hall

The TIRF imaging system achieves the best contrast and optimum resolution in x, y and z. It permits examinations of layer thicknesses of < 200 nm as well as examinations of processes at the cell membrane and in the cell-free space, in a very thin layer and in real time. 

The system provides full environmental control and allows long term time lapse in epifluorescence, TIRF, phase contrast, and DIC. 

Learn More about the ZEISS Tirf scope

Microscope: 
  • X-Cite 120Q Metal Halide Lamp
  • Motorized Scanning XY
  • Fully Enclosed Heating, CO2, and Humidity
  • DualView 2 for Simultaneous Two Channel Imaging
  • Zen2 Acquisition and Analysis Software

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
4x 0.1 Achroplan   ∞/- 11.1 Air
10x 0.45 Plan-APOCHROMAT   ∞/0.17 2 Air
20x 0.4 LD Plan Neo Phase 2, KORR, Correction Collar ∞/0-1.5 7.9 Air
20x 0.8 Plan-APOCHROMAT Phase 2 ∞/0.17 0.55 Air
40x 0.75 EC Plan-NEOFLUAR Phase 2 ∞/0.17 0.71 Air
100x 1.46 Alpha Plan-APOCHROMAT (UV)VIS-IR, DIC II ∞/0.17 0.11 Oil Immersion

Filters:

Filter Wavelength
Excitation Filter (nm) Dichoric Mirror (nm) Emission Filter (nm)
Blue Set 49 S free G365 FT 395 BP 445/50
Green Set38 HE BP 470/40 X FT 495 BP 525/50
Red Set 63 HE BP 572/25 FT 590 BP 629/62
Filter Set 74 HE DBP 480/30 + 565/25 DFT 505 + 583 DBP 525/31 + 616/57
Filter Set 50 BP 640/30 FT 660 BP 690/50

Lasers:

405 nm, 488 nm, 561 nm

Detector: 
 

Panoptiq Digital Slide Imaging System

panoptiq

Panoptiq is an interactive digital imaging system for the dynamic, real-time creation of panoramic microscope images. Unlike WSI scanners, which stitch images in a predefined pattern until completion, Panoptiq allows the user to retain control of image creation and seamlessly stitch together multiple fields of view while scanning the sample using the microscope stage. A high-frame-rate CCD camera attached directly to the user's microscope captures and transmits images to a high-performance computer, where they are processed by intuitive Panoptiq software

learn more about the Panoptiq Digital Slide Imaging System

Observe through the Z-axis

The Panoptiq™ Z module permits multi-focal and multi-objective slide review at regions of interest by allowing the user to capture Z-stacks and integrate them into digitally scanned slides. Contrary to conventional Z-stacking, which captures images at confined depth intervals, users of the add-on Z Module can rapidly capture the full range of focal depth as a high-frame-rate video with great precision. This extra Z dimension allows users to visualize the three dimensionality of structures and objects at different focal planes with respect to the slide environment.

Refocus the Image

Detect an out-of-focus area in the panoramic image? Just return to that portion of the slide and readjust the focus with your microscope - the software automatically detects a better focus and replaces the out-of-focus portion. The Panoptiq™ system preserves the best focus achieved on the panoramic image, even if the slide is observed again at a sub-optimal focus.

 

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
4x 0.16 UPlanApo -- ∞/0.17 13 Air
10x 0.3 UplanFLN IX2-DIC10 ∞/-/FN26.5   Air
20x 0.75 UPlanSApo IX2-DIC20 ∞/0.17/FN26.5   Air
40x 1.35 Uapo/340 IX2-DIC40, 1.35-0.65 Aperture ∞/0.17   Oil Immersion
60x 1.42 UPlanApo N IX2-DIC60 ∞/0.17/FN26.5 0.15 Oil Immersion
100x 1.3 UPlanFl   ∞/0.17 0.1 Oil Immersion

Nikon AZ100M Macro Fluorescence Microscope

AZ100M

Location: 1-153 Jackson Hall

  • Fluorescence (UV, CFP, GFP, YFP, dsRed and CY5 fliter sets ) and transmitted light with DIC
  • 1x and 4x PlanApo objectives
  • 1X to 8X Optical Zoom Range
  • Photometrics ES2 greyscale 12-bit camera for fluorescence
  • Nikon DS-Ri1 color 12-bit camera up to 4076x3116 pixels for full color imaging
  • Motorized X/Y stage for multi-position or panoramic images
  • Motorized Z for z-series collections for 3D and Extended Depth of Field Imaging
  • Nikon Elements AR software for multi-dimensional data collection

Learn More about the Nikon AZ100M Macro fluorescence microscope

Microscope: 

Total Magnification: 10x to 400x

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
1x 0.1 AZ PlanApo     35 mm Air
4x 0.4 AZ PlanApo     20 mm Air

Filters:

Filter Wavelengths
Excitation Filter (nm) Dichoric Mirror (nm) Emission Filter (nm)
DAPI 360-380 400 415 (LP)
CFP 426-446 (436 CWL) 455 LP 460-500 (480 CWL)
YFP 490-510 (500 CWL) 515 LP 520-550 (535 CWL)
GFP 470- 495 LP 525-
dsRed 510-555 560 LP 575-595 (580 CWL)
Detector: 

Photometrics CoolSnap ES2 12 - bit greyscale camera
1380x1024 pixels
 

Nikon Biostation Live Cell Incubator

Nikon Biostation

Locations: 1-166 Jackson Hall & 1-220B CCRB

The Nikon BioStation IM is a compact live cell incubator and imaging system that allows users to conduct live cell imaging without a steep learning curve. The BioStation IM facilitates long term time-lapse experiments, including studies of cell growth, morphology, and protein expression, by providing consistent environmental control of temperature, humidity and gas concentration in combination with phase and fluorescence imaging (blue and green excitation) of exceptional quality. Multiple positions and z-series can be collected at various magnifications from 20x to 80x (0.8na). Additional fluorescence filters sets for UV, CYP, YFP and CY5 are available, with any two possible in each experiment.

Learn more about the nikon biostation live cell incubator

There are Nikon Biostations located in both our Jackson Hall and CCRB locations.

The systems are best used with polymer or glass-bottomed 35mm dishes. Inserts are available to allow for multiple samples per experiment. Please contact UIC staff to learn more.

 
Microscope: 
  • Optical; Inverted

Methods Available :

  • Live tissue culture cell imaging
  • Time-lapse 
  • Imaging Stitching

Fluorescence Light Source

  • Metal Halide Lamp

Stage:

  • Motorized

Environmental Controls

  • Heating, CO2 and Humidity

Objectives:

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness
20x (using 0.5x optivar) 0.8   Diascopic Phase Air
40x 0.8   Diascopic Phase Air
80x (using 2x optivar) 0.8   Diascopic Phase Air

Filters:

Filter Wavelengths    
Excitation Filter (nm) Dichoric Mirror (nm) Emission Filter (nm)
GFP ET470/40 X T3951pxr ET525/50m
DsRED ET545/30 X T570LP ET620/60m

Transmitted Light-Path Filters:

  • Red LED illumination
  • Note additional fluorescence filters for UV, CFP, YFP and CY5 are available
Detector: 
  • High Sensitivity Monochrome CCD
  • 1.3 MP, 2180 x 960, 640 x 480 (binning)

Snyder Hall

Nikon Eclipse 90i

90i

Location: 23C Snyder Hall

The Nikon Eclipse 90i is a fully motorized upright microscope configured to image in transmitted light, DIC and epi-fluorescence modes. Fluorescence cubes available for DAPI, GFP, RFP, and HYQ Cy5. Fluorescence illumination is provided by a Lumencor Sola Light Engine. You can do timelapse, XYZ position, Z stacks, multiple excitation or emission wavelengths, and tiling experiments. Equipped with a Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera and a Nikon D2-Fi2 color camera running on Nikon Elements software.

Learn more about the Nikon Eclipse 90i

Objectives:

Magnification  NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
4x 0.13 Plan Fluor -- ∞/- 17.1 Air
10x 0.45 Plan Apo   ∞/0.17 4 Air
20x 0.75 Plan Apo DIC N2 ∞/0.17 1 Air
40x 0.75 Plan Fluor DIC M ∞/0.17 0.72 Air
100x 1.4 Plan Apo VC DIC N2 ∞/0.17 0.21 Oil Immersion
100x 1.3 Plan Fluor Ph3 DLL ∞/0.17 0.2 Oil Immersion

Filters:

Filter Wavelengths
Excitation Filter (nm) Dichoric Mirror (nm) Emission Filter (nm)
DAPI 325-375 (350 CWL) 400 LP 435-485 (460 CWL)

DAPI/FITC/TRITC/Cy5

Quad

380-410 (395 CWL) 410-475 (443 CWL) 425-465 (445 CWL)
475-500 (488 CWL) 505-545 (525 CWL) 505-535 (520 CWL)
540-565 (553 CWL) 575-625 (690 CWL) 575-620 (598 CWL)
625-650 (638 CWL) 655-725 (690 CWL) 670-740 (705 CWL)
GFP 450-490 (470 CWL) 495 LP 500-550 (525 CWL)
DS RED 530-560 (545 CWL) 570 LP 590-650 (620 CWL)
Cy5 590-650 (620 CWL) 660 LP 663-738 (700 CWL)

Transmitted Light Filters:

  • ND8 and ND32
  • NCB11
Lasers: 

Lumencor Sola Light Engine

Detectors: 

This instrument has two cameras:

  • Hamamatsu Orca Flash 4.0 v2 (CMOS) monochromatic Camera.
  • Nikon Color DS-Fi2 CCD Camera.

 

nikon TE2000-E Inverted fluorescence microscope

TE2000-E

Location: 23A Snyder Hall

The Nikon TE2000-E widefield inverted microscope will be capable of automated transmission and multi-color fluorescence imaging, including scanning of slides and plates, with Nikon Elements v5.02 and JOBS software. The scope will be equipped for DAPI, GFP, YFP, dsRed, and optional CFP and far-red probes. The scope will be suited for multi-well plate and slide imaging with motorized x/y stage and z-motor. We have been running the current scope using Nikon Elements for some time but will be expanding the software capabilities to accomplish fully automated image acquisition and analysis using Nikon JOBS. This microscope is equipped with a Hamamatsu ORCA Flash 4.0 grayscale camera for samples where more sensitivity is needed.

Learn more about the nikon TE2000-E Inverted fluorescence microscope

Microscope: 

ProsScan III motorized XY stage and built in Z motor.
Remote controller

Objectives:

Magnification  NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
1x 0.04 Plan UW   ∞/- 3.2 Air
2x 0.1 Plan Apo   ∞/- 8.5 Air
10x 0.3 Plan Fluor Ph1 DL ∞/1.2 15.2 Air
20x 0.45 S Plan Fluor Ph1 ADM, Correction Collar ∞/0-2 8.2-6.9 ELWD Air
40x 0.6 S PlanFluor Ph2 ADM, Correction Collar ∞/0-2 3.6-2.8 ELWD Air

Filters:

Filter Wavelengths
Excitation Filter (nm) Dichoric Mirror (nm) Emission Filter (nm)
ET DAPII 350/50 400 LP 460/50
ET GFP 470/40 495 LP 525/50
HQ R HQ545/30 Q570 LP HQ620/60
ET Cy5 (optional) ET 620/60 660 LP ET 700/75
CY GFP (optional) 436/20 455 LP 480/40
ET YFP 500/20 515 LP 535/30
Lasers: 

Solar Fluorescent light engine (Full Spectrum LED)
No lasers.

Detector: 

Hamamatsu Orca Flash 4
16 bit monochrome 
2048 x 2044 with 2x2 or 4x4 binning