Light Microscopes

Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected for subsequent molecular analysis.

• Training video using the LMD6500 on JOVE
• LDM works best when using PEN membrane slides. The PEN membrane on glass slides are here
• Videos on the LDM technique can be found here

Transmitted Light Filters

• ND2, ND4, ND8, and ND16, Green and Cool Blue

Lasers

• Wavelength: 355 nm, Max. pulse energy: 70 µJ, Pulse frequency: 80 Hz

Detector

• LMD CC7000 color camera

Objectives

Filters

Sort, manipulate, and image your specimens with the stereo objective in 3D, with large fields of view, large working distances, and an outstanding depth of field. Simply switch to the HR (High Resolution) micro-objective and analyze the quality of the gene expression immediately and without delay via an optical light microscope at a resolution of 0.7 µm (1320 pairs of lines/mm) and an 800x magnification.

Detector

Leica DFC420 C camera. Digital 5 MP color camera with peltier cooling system to capture high-resolution images for analysis, documentation, and presentation.

Objectives

Filters

The Nikon Ti2 motorized inverted microscope features include Nikon’s patented PFS Perfect Focus III focus lock technology for objectives at 4x, 10x, 20x 20xW, 40x oil, 60x oil, and 100x oil magnifications. The Spectra X light engine has wavelengths tailored to most dyes, including Opal dyes. The Prime 95 camera and triggered light source allow for rapid automated imaging using JOBS, deconvolution, time measurements, and automated collection of slides, dishes, and plates. The system has a 6-slide holder for automated scanning.

The Nikon Ti-E Motorized Microscope with Ibidi live cell environment chamber features include Nikon’s patented PFS Perfect Focus III focus lock technology. 100 Watt Illumination system for transmitted and LED-reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 40x* NAMC Contrast, 60x, and 100x DIC magnifications (*correction collars). Fluorescence accessories for widefield applications for DAPI, GFP, CFP, YFP, RFP and Cy5...and many others. JOBS, deconvolution, time measurements, automated collection of slides, dishes, and plates.

SPECTRA III LED light source lines (nm): 365, 440, 488, 514, 561, 594*, 640, 730*

*New!

Resources

• How to adjust objectives with correction collars
• How deconvolution can improve optical image data

Live Cell

• Live cell imaging reagents
• Ibidi Environmental Stage with CO2 and O2 regulation
• Ibidi dishes and chambered slides
• We also have the Braintree Scientific Automated Syringe pump to perfuse solutions! Holds 6 syringes up to 50cc.

Objectives

Fluorescence (UV, CFP, GFP, YFP, dsRed, and CY5 filter sets ) and transmitted light with DIC

• 1x and 4x PlanApo objectives
• 1X to 8X Optical Zoom Range
• Photometrics ES2 greyscale 12-bit camera for fluorescence
• Nikon DS-Ri1 color 12-bit camera up to 4076x3116 pixels for full-color imaging
• Motorized X/Y stage for multi-position or panoramic images
• Motorized Z for z-series collections for 3D and Extended Depth of Field Imaging
• Nikon Elements AR software for multi-dimensional data collection
• Total Magnification: 10x to 400x

Detector

• Photometrics CoolSnap ES2 12-bit greyscale camera
• 1380x1024 pixels, Nikon DS-Ri1 CCD 12-bit color camera
• Up to 4076x3116 pixels

Objectives

Filters

The Zeiss Widefield and TIRF imaging system achieves the best contrast and optimum resolution in x, y, and z. It permits examinations of layer thicknesses of < 200 nm as well as examinations of processes at the cell membrane and in the cell-free space, in a very thin layer, and in real-time. 

The system provides full environmental control and allows long-term time-lapse in epifluorescence, TIRF, phase contrast, and DIC. 

Microscope

• HXP 120C Mercury Vapor Lamp
• Motorized Tiled Scanning XYZ
• Fully Enclosed Heating, CO2, and Humidity
• DualView 2 for Simultaneous two-channel Imaging
• Zen Blue Acquisition and Analysis Software

Lasers

• 488 nm, 561 nm

Detector 

• Photometrics QuantEM 512SC - High Sensitivity 16 bit, 512 x 512 grayscale EMCCD
• AxioCam MRm - 1388 x 1040, 12 bit grayscale CCD
• DualView 2 for simultaneous two-channel imaging

Objectives

Filters

The Incucyte SX5 Live-Cell Analysis System is a real-time cell monitoring and surveillance system that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside a standard tissue culture incubator.

Vessel Compatibility

Your cells must be in a compatible vessel to utilize the Incucyte SX5. Please review the list below to see if your vessel is compatible.

UIC Incucyte SX5 compatible vessels

System Features:

• 3 interchangeable vessel trays for over 600 vessels types, with up to 6 microplates in the instrument for parallel for unattended time-lapse live cell imaging
• Up to three fluorescence channels (Green/Orange/Near IR) plus phase contrast images collected in a single experiment. Includes a long wavelength, low phototoxicity NIR channel with specific reagents available for all channels for assays in apoptosis, cell proliferation, cytotoxicity, spheroids, and cell and organellar cycles.
• 4x, 10x, and 20x objectives on an automated turret
• 3 interchangeable vessel trays for over 600 vessels types, with up to 6 microplates in the instrument for parallel for unattended time-lapse live cell imaging

The Nikon Eclipse 90i is a fully motorized upright microscope configured to image in transmitted light, DIC, and epi-fluorescence modes. Fluorescence cubes are available for DAPI, GFP, RFP, and HYQ Cy5. Fluorescence illumination is provided by a Lumencor Sola Light Engine. You can do timelapse, XYZ position, Z stacks, multiple excitations or emission wavelengths, and tiling experiments. Equipped with a Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera and a Nikon D2-Fi2 color camera running on Nikon Elements software.

Transmitted Light Filters

• ND8, ND32, and NCB11

Lasers 

Lumencor Sola Light Engine

Detectors

This instrument has two cameras:

• Hamamatsu Orca Flash 4.0 v2 (CMOS) monochromatic Camera.
• Nikon Color DS-Fi2 CCD Camera.

Objectives

Filters