Light Microscopes

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Cancer and Cardiovascular Research Building (CCRB)

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Cancer and Cardiovascular Research Building (CCRB)

Leica LMD6500 Laser Dissection Microscope

uic_lmd6500

Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected by software, excised from the surrounding tissue by a laser, and collected for subsequent molecular analysis.

 

Transmitted Light Filters

  • ND2, ND4, ND8, and ND16, Green and Cool Blue

Lasers

  • Wavelength: 355 nm, Max. pulse energy: 70 µJ, Pulse frequency: 80 Hz

Detector

  • LMD CC7000 color camera

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

1.25

0.04

HCX FL FLUOTAR

 

∞/-/^

3.7

 

5x

0.12

UVI

 

∞/-

11.7

Air

10x

0.3

HCX FL FLUOTAR

Ph2

∞/-/D

11

Air

20x

0.4

HCX FL FLUOTAR

Ph1

∞/0-2/C

6.9

Air

40x

0.6

HCX FL FLUOTAR

Corr XT

∞/0-2/C

3.3-1.9

Air

63x

0.7

HCX FL FLUOTAR

Ph2

∞/0.1-1.3/C

2.6

Air

 

Filters

Filter

Excitation Filter (nm)

Dichoric Mirror (nm)

Emission Filter (nm)

Filter Wheel Position

DAPI

360-370 (365 CWL)

380 LP

420 LP

Open

U/B/G (triple DAPI/GFP/dsRed)

385-415 (405 CWL)

410-470 (440 CWL)

450-470 (460 CWL)

UV/B/G

 

Leica MZ FL III Fluorescence Stereomicroscope

leica_mz_iii

Sort, manipulate, and image your specimens with the stereo objective in 3D, with large fields of view, large working distances, and an outstanding depth of field. Simply switch to the HR (High Resolution) micro-objective and analyze the quality of the gene expression immediately and without delay via an optical light microscope at a resolution of 0.7 µm (1320 pairs of lines/mm) and an 800x magnification.

 

Detector

Leica DFC420 C camera. Digital 5 MP color camera with peltier cooling system to capture high-resolution images for analysis, documentation, and presentation.

 

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

0.5x

 

Plan

 

139

Air

1x

0.035

PlanApo

-/-

55

Air

2x

0.07

PlanApo

-/-

15

Air

 

Filters

Filter

Wavelengths 

Excitation Filter (nm)

Emission Filter (nm)

UV

D350/50x

E420 1pv2

GFP1 

ET425/60x

ET505/40m

GFP2 

480/40

510

546/10

590

 

Nikon Ti2 Microscope

uic_ti2

The Nikon Ti2 motorized inverted microscope features include Nikon’s patented PFS Perfect Focus III focus lock technology for objectives at 4x, 10x, 20x 20xW, 40x oil, 60x oil, and 100x oil magnifications. The Spectra X light engine has wavelengths tailored to most dyes, including Opal dyes. The Prime 95 camera and triggered light source allow for rapid automated imaging using JOBS, deconvolution, time measurements, and automated collection of slides, dishes, and plates. The system has a 6-slide holder for automated scanning.

Nikon Ti-E Inverted Deconvolution Microscope

uic_tie

The Nikon Ti-E Motorized Microscope with Ibidi live cell environment chamber features include Nikon’s patented PFS Perfect Focus III focus lock technology. 100 Watt Illumination system for transmitted and LED-reflected light applications. New Lambda coat anti-reflective coated objectives at 4x, 10x, 20x, 40x* NAMC Contrast, 60x, and 100x DIC magnifications (*correction collars). Fluorescence accessories for widefield applications for DAPI, GFP, CFP, YFP, RFP and Cy5...and many others. JOBS, deconvolution, time measurements, automated collection of slides, dishes, and plates.

 

SPECTRA III LED light source lines (nm): 365, 440, 488, 514, 561, 594*, 640, 730*

*New!

 

Resources

Live Cell

Objectives

Magnification NA Correction Optic Properties Tube Length/Cover Glass Thickness Working Distance (mm) Immersion Medium
4x 0.13 PlanFluor -- ∞/- 17.1 Air
10x 0.45 PlanApo DIC N1 ∞/0.17 4.0 Air
20x 0.75 PlanApo DIC M/N2 ∞/0.17 1.0 Air
40x 0.95 Plan Apo DIC MN2, Correction Collar ∞/0.11-0.23 0.14 Air
60x* 1.20 Plan Apo VC with correction collar   ∞/0.15-0.18 0.27 Water
60x 1.4 PlanApo DIC N2 ∞/0.17 0.13 Oil
100x 1.45 PlanApo DIC N2 ∞/0.17 0.13 Oil

Jackson Hall

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Jackson Hall

Nikon AZ100M Macro Fluorescence Microscope

uic_az100m

Fluorescence (UV, CFP, GFP, YFP, dsRed, and CY5 filter sets ) and transmitted light with DIC

  • 1x and 4x PlanApo objectives
  • 1X to 8X Optical Zoom Range
  • Photometrics ES2 greyscale 12-bit camera for fluorescence
  • Nikon DS-Ri1 color 12-bit camera up to 4076x3116 pixels for full-color imaging
  • Motorized X/Y stage for multi-position or panoramic images
  • Motorized Z for z-series collections for 3D and Extended Depth of Field Imaging
  • Nikon Elements AR software for multi-dimensional data collection
  • Total Magnification: 10x to 400x

Detector

  • Photometrics CoolSnap ES2 12-bit greyscale camera
  • 1380x1024 pixels, Nikon DS-Ri1 CCD 12-bit color camera
  • Up to 4076x3116 pixels

Objectives

Magnification

NA

Correction

Working Distance (mm)

Immersion Medium

1x

0.1

AZ PlanApo

35 mm

Air

4x

0.4

AZ PlanApo

20 mm

Air

 

Filters

Filter

Excitation Filter (nm)

Dichroic Mirror (nm)

Emission Filter (nm)

DAPI

360-380

400

415 (LP)

CFP

426-446 (436 CWL)

455 LP

460-500 (480 CWL)

YFP

490-510 (500 CWL)

515 LP

520-550 (535 CWL)

GFP

470-

495 LP

525-

dsRed

510-555

560 LP

575-595 (580 CWL)

Zeiss Widefield and TIRF Microscope

uic_zeiss_tirf

The Zeiss Widefield and TIRF imaging system achieves the best contrast and optimum resolution in x, y, and z. It permits examinations of layer thicknesses of < 200 nm as well as examinations of processes at the cell membrane and in the cell-free space, in a very thin layer, and in real-time. 

The system provides full environmental control and allows long-term time-lapse in epifluorescence, TIRF, phase contrast, and DIC. 

Microscope

  • HXP 120C Mercury Vapor Lamp
  • Motorized Tiled Scanning XYZ
  • Fully Enclosed Heating, CO2, and Humidity
  • DualView 2 for Simultaneous two-channel Imaging
  • Zen Blue Acquisition and Analysis Software

Lasers

  • 488 nm, 561 nm

Detector 

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

4x

0.1

Achroplan

 

∞/-

11.1

Air

10x

0.45

Plan-APOCHROMAT

 

∞/0.17

2

Air

20x

0.4

LD Plan Neo

Phase 2, KORR, Correction Collar

∞/0-1.5

7.9

Air

20x

0.8

Plan-APOCHROMAT

Phase 2

∞/0.17

0.55

Air

40x

0.75

EC Plan-NEOFLUAR

Phase 2

∞/0.17

0.71

Air

100x

1.46

Alpha Plan-APOCHROMAT

(UV)VIS-IR, DIC II

∞/0.17

0.11

Oil

 

Filters

Filter

Wavelength

Excitation Filter (nm)

Dichroic Mirror (nm)

Emission Filter (nm)

Blue Set 49 S free

G365

FT 395

BP 445/50

Green Set38 HE

BP 470/40 X

FT 495

BP 525/50

Red Set 63 HE

BP 572/25

FT 590

BP 629/62

Filter Set 74 HE

DBP 480/30 + 565/25

DFT 505 + 583

DBP 525/31 + 616/57

Filter Set 50

BP 640/30

FT 660

BP 690/50

Sartorius Incucyte SX5

uic_incucyte_sx5

The Incucyte SX5 Live-Cell Analysis System is a real-time cell monitoring and surveillance system that automatically captures and analyzes images of living cells around-the-clock for days, weeks, or months, while cells remain undisturbed inside a standard tissue culture incubator.

 

Vessel Compatibility

Your cells must be in a compatible vessel to utilize the Incucyte SX5. Please review the list below to see if your vessel is compatible.

UIC Incucyte SX5 compatible vessels

System Features:

  • 3 interchangeable vessel trays for over 600 vessels typeswith up to 6 microplates in the instrument for parallel for unattended time-lapse live cell imaging
  • Up to three fluorescence channels (Green/Orange/Near IR) plus phase contrast images collected in a single experiment. Includes a long wavelength, low phototoxicity NIR channel with specific reagents available for all channels for assays in apoptosis, cell proliferation, cytotoxicity, spheroids, and cell and organellar cycles.
  • 4x, 10x, and 20x objectives on an automated turret
  • 3 interchangeable vessel trays for over 600 vessels typeswith up to 6 microplates in the instrument for parallel for unattended time-lapse live cell imaging

 

Snyder Hall

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Snyder Hall

Nikon 90i

uic_90i

The Nikon Eclipse 90i is a fully motorized upright microscope configured to image in transmitted light, DIC, and epi-fluorescence modes. Fluorescence cubes are available for DAPI, GFP, RFP, and HYQ Cy5. Fluorescence illumination is provided by a Lumencor Sola Light Engine. You can do timelapse, XYZ position, Z stacks, multiple excitations or emission wavelengths, and tiling experiments. Equipped with a Hamamatsu Orca Flash 4.0 v2 CMOS monochrome camera and a Nikon D2-Fi2 color camera running on Nikon Elements software.

 

Transmitted Light Filters

  • ND8, ND32, and NCB11

Lasers 

Lumencor Sola Light Engine

Detectors

This instrument has two cameras:

  • Hamamatsu Orca Flash 4.0 v2 (CMOS) monochromatic Camera.
  • Nikon Color DS-Fi2 CCD Camera.

Objectives

Magnification

NA

Correction

Optic Properties

Tube Length/Cover Glass Thickness

Working Distance (mm)

Immersion Medium

4x

0.13

Plan Fluor

--

∞/-

17.1

Air

10x

0.45

Plan Apo

 

∞/0.17

4

Air

20x

0.75

Plan Apo

DIC N2

∞/0.17

1

Air

40x

0.75

Plan Fluor

DIC M

∞/0.17

0.72

Air

100x

1.4

Plan Apo VC

DIC N2

∞/0.17

0.21

Oil

100x

1.3

Plan Fluor

Ph3 DLL

∞/0.17

0.2

Oil

 

Filters

Filter

Excitation Filter (nm)

Dichoric Mirror (nm)

Emission Filter (nm)

DAPI

325-375 (350 CWL)

400 LP

435-485 (460 CWL)

DAPI/FITC/TRITC/Cy5

Quad

380-410 (395 CWL)

410-475 (443 CWL)

425-465 (445 CWL)

475-500 (488 CWL)

505-545 (525 CWL)

505-535 (520 CWL)

540-565 (553 CWL)

575-625 (690 CWL)

575-620 (598 CWL)

625-650 (638 CWL)

655-725 (690 CWL)

670-740 (705 CWL)

GFP

450-490 (470 CWL)

495 LP

500-550 (525 CWL)

DS RED

530-560 (545 CWL)

570 LP

590-650 (620 CWL)

Cy5

590-650 (620 CWL)

660 LP

663-738 (700 CWL)