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Small Animal Imaging

Cancer & Cardiovascular Research Building (CCRB)

Sofie G8 PET/CT

Sofie G8

The Sofie G8 PET/CT high-performance benchtop PET/CT imaging system offers enhanced image quality, proper animal care and safety, and increased study volume.

Employing high-resolution detectors, fast electronics, and advanced 3D image reconstruction, G8 PET / CT’s small size does not sacrifice performance (14% sensitivity, 1.4 mm resolution) or image quality.

Learn more about the Sofie G8 PET/CT

RESOLUTION

  • 200um reconstructed CT
  • 457um PET 

G8 CT Specifications

X-ray Source 59 kVp, 100uA
X-ray Camera 75 micron pixels, 14bits, CMOS+Csl flat panel
Camera Speed 24 fps
Field of View 100mm axial by 50mm transaxial
Standard Scan Time <60 seconds
Dimensions 56 cm x 63 cm x 71 cm (W x D x H)
Weight 90 kg

G8 PET Performance Specifications

Axial FOV 9.5 cm
Transaxial FOV 4.7 cm
Detector element size 1.8 mm x 1.8 mm x 7 mm
Peak absolute system sensitivity* >14%
Reconstructed resolution at center of FOV* 1.4 mm
Average energy resolution ≤18%
Energy window range 150 - 650 keV
Total number of detector elements 5,408
Reconstruction algorithm 3D ML-EM

Publication: Performance Evaluation of G8, a High-Sensitivity Benchtop Preclinical PET/CT Tomograph

IVIS 100 In vivo Imaging System

ivis100

Location: 1-260C CCRB

The IVIS 100 Imaging System is a highly sensitive and large field of view imaging system that offers users the flexibility to image bioluminescent reporters both in vivo and in vitro. It relies on patented optical imaging technologies to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

Learn more about the IVIS 100 In vivo Imaging System

The IVIS offers an adjustable field of view of 10-25 cm, allowing 5 mice or 2 large rats to be imaged. The system features a 25 mm (1.0 inch) square back-thinned, back-illuminated CCD, which is cryogenically cooled to –90°C via a closed cycle refrigeration system (without liquid nitrogen) to minimize electronic background and maximize sensitivity. A patented and extremely light-tight, low background imaging chamber allows the IVIS to be used in standard lab lighting environments. The system includes animal handling features such as a heated sample shelf, gas anesthesia connections, and an optional full gas anesthesia system. The IVIS Imaging System is highly automated with all hardware motor movement, imaging parameters, and image analysis controlled via Living Image® software.

Additional Capabilities:

  • Anesthetics (isoflurane) 
  • Purifier Class II Bio-safety cabinet 
  • Post Processing Analysis
Detector: 
  • Spectral Instruments (Roper) Back Illuminated Grade 1 CCD, cooled to -105 degrees C.
  • 1.74 mega pixels (20 micron pixel size)
  • 21.5 cm maximum image width (up to 5 mice)

Vevo 2100

vevo2100

Locations: 1-260D CCRB & 1-161 Jackson Hall

Ultra High Frequency ultrasound for small animal research has linear array technology, ideal for cardiovascular and cancer imaging.

The MicroScan transducers provide increased frame rates, superb contrast, unrivaled resolution and a wider field of view.

Learn more about the Vevo 2100

Available Imaging options for the Vevo 2100 system:

  • Super B-Mode (2D) imaging for anatomical visualization and quantification, with enhanced temporal resolution with frame rates up to 740 fps (in 2D for a 4x4 mm FOV) , and enhanced image uniformity with multiple focal zones.
  • M-Mode for visualization and quantification of wall motion in cardiovascular research, single line acquisition allows for the very high-temporal (1000fps) resolution necessary for analysis of LV function.
  • Anatomical M-Mode for adjustable anatomical orientation in reconstructed M-Mode imaging; software automatically optimizaes field of view for maxiumum frame rate.
  • Pulsed-Wave Doppler Mode (PW) for quantification of blood flow.
  • Color Doppler Mode for detection of blood vessels including flow directional information and mean velocities; as well as for indentification of small vessels not visible in B-Mode.
  • Power Doppler Mode for detection and quantification of blood flow in small vessels not visible in B-Mode; increased frame rates allow for significantly faster data acquisition. 
  • Tissue Doppler Mode for quantification of myocardial tissue movement; for example in assessing diastolic dysfunction.
  • Vevo MicroMarker Nonlinear Contrast Agent Imaging - for quantification of relative perfusion & molecular expression of endothelial cell surface markers; enhanced sensitivity to Vevo MicroMarker contrast agents as linear tissue signal is suppressed. 
  • 3D-Mode Imaging for anatomical and vascular visualization, when combined with either B-Mode, Power Doppler Mode or Nonlinear Contract Imaging; allows for quantification of volume and vascularity within a defined anatomical structure.
  • Digital RF-Mode for the acquisition and exportation of radio frequency (RF) data in digital format for further analysis; full screen acquisition provides a complete data set for more comprehensive analysis and tissue characterization.

Jackson Hall

IVIS Spectrum In vivo Imaging System

Spectrum

Location: 1-166A Jackson Hall

The IVIS Spectrum is the most versatile and advanced in vivo imaging system available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Learn more about the IVIS Spectrum In vivo Imaging System

Additional details, specifications and vendor protocols can be found on the PerkinElmer site.

Detector: 
  • Andor Back-thinned, Back-illuminated Grage1 CCD, Peltier cooled to -90 degrees C
  • 2048 x 2048 Pixels Array, 13.5 Micron Pixel Size
  • 21.5 cm maximum image width (up to 5 mice)

Excitation:

  • 430-745nm at 35nm increments (10 filters)
  • ND2 Filter

Emission:

10 excitation filters with 35 nm bandwidth, 18 emission filters with 20 nm bandwidth.

Excitation Emission
430 500
465 520
500 540
535 560
570 580
605 600
640 620
675 640
710 660
745 680
  700
  720
  740
  760
  780
  800
  820
  840

Vevo 2100

vevo 2100 jh

Locations: 1-260D CCRB & 1-161 Jackson Hall

High-frequency, high-resolution digital imaging platform with linear array technology and color doppler mode.

Learn more about the Vevo 2100

Available Imaging options for the Vevo 2100 system:

  • Super B-Mode (2D) imaging for anatomical visualization and quantification, with enhanced temporal resolution with frame rates up to 740 fps (in 2D for a 4x4 mm FOV) , and enhanced image uniformity with multiple focal zones.
  • M-Mode for visualization and quantification of wall motion in cardiovascular research, saingle line acquisition allows for the very high-temporal (1000fps) resolution necessary for analysis of LV function.
  • Anatomical M-Mode for adjustable anatomical orientation in reconstructed M-Mode imaging; software automatically optimizaes field of view for maxiumum frame rate.
  • Pulsed-Wave Doppler Mode (PW) for quantification of blood flow
  • Color Doppler Mode for detection of blood vessels including flow directional information and mean velocities; as well as for indentification of small vessels not visible in B-Mode.
  • Power Doppler Mode for detection and quantification of blood flow in small vessels not visible in B-Mode; increased frame rates allow for significantly faster data acquisition. 
  • Tissue Doppler Mode for quantification of myocardial tissue movement; for example in assessing diastolic dysfunction
  • Vevo MicroMarker Nonlinear Contrast Agent Imaging - for quantification of relative perfusion & molecular expression of endothelial cell surface markers; enhanced sensitivity to Vevo MicroMarker contrast agents as linear tissue signal is suppressed. 
  • 3D-Mode Imaging for anatomical and vascular visualization, when combined with either B-Mode, Power Doppler Mode or Nonlinear Contract Imaging; allows for quantification of volume and vasularity within a defined anatomical structure
  • Digital RF-Mode for the acquisition and exportation of radio frequency (RF) data in digital format for further analysis; full screen acquisition provides a complete data set for more comprehensive analysis and tissue characterization.