Small Animal Imaging

The IVIS Spectrum is the most versatile and advanced optical animal in vivo imaging system available on the market today. An optimized set of high-efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near-infrared wavelength region. It also offers 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using the Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans-illumination fluorescent images. The instrument is equipped with 10 narrow-band excitation filters (30 nm bandwidth) and 18 narrow-band emission filters (20 nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Additional details, specifications, and vendor protocols can be found on the PerkinElmer site.

Detector 

• Andor Back-thinned, Back-illuminated CCD, cooled to -90 degrees C
• 2048 x 2048 Pixels Array, 13.5-micron sensor size
• 21.5 cm maximum image width (up to 5 mice)

Excitation

• 430-745 nm (10 filters)
• ND2 filter for trans-illumination

Emission

10 excitation filters with 30 nm bandwidth, and 18 emission filters with 20 nm bandwidth. The central filter wavelength is listed.

The Sofie G8 PET/CT high-performance benchtop PET/CT imaging system offers enhanced image quality, proper animal care, safety, and increased study volume.

Employing high-resolution detectors, fast electronics, and advanced 3D image reconstruction, G8 PET / CT’s small size does not sacrifice performance (14% sensitivity, 1.4 mm resolution) or image quality.

RESOLUTION

• 200um reconstructed CT
• 457um PET

G8 CT Specifications

G8 PET Performance Specifications

Publication: Performance Evaluation of G8, a High-Sensitivity Benchtop Preclinical PET/CT Tomograph

The IVIS 100 Imaging System is a highly sensitive and large field-of-view imaging system that offers users the flexibility to image bioluminescent reporters both in vivo and in vitro. It relies on patented optical imaging technologies to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking, and gene expression patterns in living animals.

The IVIS offers an adjustable field of view of 10-25 cm, allowing 5 mice or 2 large rats to be imaged. The system features a 25 mm (1.0 inch) square back-thinned, back-illuminated CCD, which is cryogenically cooled to –90°C via a closed cycle refrigeration system (without liquid nitrogen) to minimize electronic background and maximize sensitivity. A patented and extremely light-tight, low background imaging chamber allows the IVIS to be used in standard lab lighting environments. The system includes animal handling features such as a heated sample shelf, gas anesthesia connections, and an optional full gas anesthesia system. The IVIS Imaging System is highly automated with all hardware motor movement, imaging parameters, and image analysis controlled via Living Image® software.

Additional Capabilities:

• Anesthetics (isoflurane) 
• Purifier Class II Bio-safety cabinet 
• Post Processing Analysis

Detector: 

• Spectral Instruments (Roper) Back Illuminated Grade 1 CCD, cooled to -105 degrees C.
• 1.74 megapixels (20-micron pixel size)
• 21.5 cm maximum image width (up to 5 mice)

Ultra High-Frequency ultrasound for small animal research has linear array technology, ideal for cardiovascular and cancer imaging.

The MicroScan transducers provide increased frame rates, superb contrast, unrivaled resolution and a wider field of view.

Available Imaging options for the Vevo 2100 system:

• Super B-Mode (2D) imaging for anatomical visualization and quantification, with an enhanced temporal resolution with frame rates up to 740 fps (in 2D for a 4x4 mm FOV) , and enhanced image uniformity with multiple focal zones.

• M-Mode for visualization and quantification of wall motion in cardiovascular research, single line acquisition allows for the very high-temporal (1000fps) resolution necessary for the analysis of LV function.
• Anatomical M-Mode for adjustable anatomical orientation in reconstructed M-Mode imaging; the software automatically optimizes the field of view for maximum frame rate.
• Pulsed-Wave Doppler Mode (PW) for quantification of blood flow.
• Color Doppler Mode for detection of blood vessels including flow directional information and mean velocities; as well as for identification of small vessels not visible in B-Mode.
• Power Doppler Mode for detection and quantification of blood flow in small vessels not visible in B-Mode; increased frame rates allow for significantly faster data acquisition. 
• Tissue Doppler Mode for quantification of myocardial tissue movement; for example in assessing diastolic dysfunction.
• Vevo MicroMarker Nonlinear Contrast Agent Imaging - for quantification of relative perfusion & molecular expression of endothelial cell surface markers; enhanced sensitivity to Vevo MicroMarker contrast agents as linear tissue signal is suppressed. 
• 3D-Mode Imaging for anatomical and vascular visualization, when combined with either B-Mode, Power Doppler Mode, or Nonlinear Contract Imaging; allows for quantification of volume and vascularity within a defined anatomical structure.
• Digital RF-Mode for the acquisition and exportation of radio frequency (RF) data in digital format for further analysis; fullscreen acquisition provides a complete data set for more comprehensive analysis and tissue characterization.

The IVIS Spectrum is the most versatile and advanced optical animal in vivo imaging system available on the market today. An optimized set of high-efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near-infrared wavelength region. It also offers 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using the Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans-illumination fluorescent images. The instrument is equipped with 10 narrow-band excitation filters (30 nm bandwidth) and 18 narrow-band emission filters (20 nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

Additional details, specifications, and vendor protocols can be found on the PerkinElmer site.

Detector 

• Andor Back-thinned, Back-illuminated CCD, cooled to -90 degrees C
• 2048 x 2048 Pixels Array, 13.5-micron sensor size
• 21.5 cm maximum image width (up to 5 mice)

Excitation

• 430-745 nm (10 filters)
• ND2 filter for trans-illumination

Emission

10 excitation filters with 30 nm bandwidth, and 18 emission filters with 20 nm bandwidth. The central filter wavelength is listed.

Ultra High-Frequency ultrasound for small animal research has linear array technology, ideal for cardiovascular and cancer imaging.

The MicroScan transducers provide increased frame rates, superb contrast, unrivaled resolution and a wider field of view.

Available Imaging options for the Vevo 2100 system:

• Super B-Mode (2D) imaging for anatomical visualization and quantification, with an enhanced temporal resolution with frame rates up to 740 fps (in 2D for a 4x4 mm FOV) , and enhanced image uniformity with multiple focal zones.

• M-Mode for visualization and quantification of wall motion in cardiovascular research, single line acquisition allows for the very high-temporal (1000fps) resolution necessary for the analysis of LV function.
• Anatomical M-Mode for adjustable anatomical orientation in reconstructed M-Mode imaging; the software automatically optimizes the field of view for maximum frame rate.
• Pulsed-Wave Doppler Mode (PW) for quantification of blood flow.
• Color Doppler Mode for detection of blood vessels including flow directional information and mean velocities; as well as for identification of small vessels not visible in B-Mode.
• Power Doppler Mode for detection and quantification of blood flow in small vessels not visible in B-Mode; increased frame rates allow for significantly faster data acquisition. 
• Tissue Doppler Mode for quantification of myocardial tissue movement; for example in assessing diastolic dysfunction.
• Vevo MicroMarker Nonlinear Contrast Agent Imaging - for quantification of relative perfusion & molecular expression of endothelial cell surface markers; enhanced sensitivity to Vevo MicroMarker contrast agents as linear tissue signal is suppressed. 
• 3D-Mode Imaging for anatomical and vascular visualization, when combined with either B-Mode, Power Doppler Mode, or Nonlinear Contract Imaging; allows for quantification of volume and vascularity within a defined anatomical structure.
• Digital RF-Mode for the acquisition and exportation of radio frequency (RF) data in digital format for further analysis; fullscreen acquisition provides a complete data set for more comprehensive analysis and tissue characterization.