New Collaborative Frontier
Spatialomics technology is an exciting new collaborative frontier between the UIC and UMGC that will provide new avenues for researchers to increase their understanding of cells in their morphological context. The relationship between cells and their relative locations within a tissue sample can be critical to understanding disease pathology. Spatialomics allows scientists to measure all the gene activity in a tissue sample and map where the activity is occurring. Already this technology is leading to new discoveries that will prove instrumental in helping scientists gain a better understanding of biological processes and disease.
Multiplexed Ion Beam Imaging (MIBI)
In addition to the collaborative Spatialomic platforms, the University Imaging Centers has also installed a Multiplexed Ion Beam Imaging system (MIBI).
The MIBIscope System is a revolutionary imaging platform, enabling comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment. The MIBIscope allows researchers to visualize over 40 markers simultaneously with higher sensitivity, resolution, and throughput than existing methods.
nanoString: GeoMx Digital Spatial Profiling (DSP)
NanoString’s GeoMx Digital Spatial Profiler (DSP) combines standard immunofluorescence techniques with digital optical barcoding technology to perform highly multiplexed, spatially resolved profiling experiments. In a single reaction, the DSP technology performs whole slide imaging with up to four fluorescent stains to capture tissue morphology and select regions of interest for high plex profiling. The ability to perform tissue morphology guided profiling experiments increases the likelihood of capturing rare events often missed by typical grind and bind assays. The DSP chemistry enables spatially resolved high plex profiling of RNA and protein targets on just two serial sample sections. The ability to perform multianalyte analysis enables deep characterization of the sample.
10x Genomics: Visium Spatial Gene Expression
Visium Spatial Gene Expression measures either the whole transcriptome or a defined set of transcripts in intact tissue sections and maps where gene activity is occurring. With whole transcriptome analysis envision the spatial organization of newly discovered cell types, states, and biomarkers. To validate your discoveries or hone in on all relevant genes and pathways, pair Visium with pre-designed oncology, immunology, or neuroscience targeted gene panels, or design your own custom panels. Visium can also be combined with immunofluorescence for simultaneous visualization of protein and gene expression.