Light Sheet Microscope

The Cleared Tissue LightSheet (CTLS) is a large field light-sheet microscope designed to image whole organs at high speed. CTLS creates a focused sheet with a narrow waist for better optical sectioning, then uses a spatial light modulator (SLM) to rapidly shift the waist of the sheet along the axis of propagation. A dual excitation setup allows imaging from the right and left sides of the specimen for optimal light-sheet projection throughout. Piezoelectric stages move the specimen in x, y, and z with sub-micron resolution. The result is clear: a 1 cm3 volume can be imaged at up to 1µm x 1µm x 3µm (XYZ) resolution. A cleared mouse brain can be imaged in as little as 10 minutes at 6.2µm x 6.2µm x 10µm (XYZ).

• The thickness of the sheet can range from 20µm to 2µm depending on the number of tiles
• The sample moves to theoretically image samples just under 1cm³ in size

Lasers

• 488 nm, 561 nm, 515 nm, 640 nm

Detector 

• Hamamatsu Flash 4 v3

Objectives