Light Sheet Microscopes
Jackson Hall
3i cleared tissue Lightsheet microscope

Location: 2-168 Jackson Hall
Cleared Tissue LightSheet (CTLS) is a large field light-sheet microscope designed to image whole organs at high speed. CTLS creates a focused sheet with a narrow waist for better optical sectioning, then uses a spatial light modulator (SLM) to rapidly shift the waist of the sheet along the axis of propagation. A dual excitation setup allows imaging from the right and left sides of the specimen for optimal light-sheet projection throughout. Piezoelectric stages move the specimen in x, y, and z with sub-micron resolution. The result is clear: a 1 cm3 volume can be imaged at up to 1µm x 1µm x 3µm (XYZ) resolution. A cleared mouse brain can be imaged in as little as 1.5 hours at 25µm x 25µm x 25µm (XYZ).
Learn more about the 3i cleared tissue Lightsheet microscope
Need a custom sample chamber? Visit fireflysci cuvette shop.
Magnification | NA | Correction | Optic Properties | Tube Length/Cover Glass Thickness | Working Distance (mm) |
1.5 | 0.37 | 30 | |||
5X | 0.14 | MPlan Apo | ∞/0 | F=200 | |
5X | 0.14 | Mituitoyo | ∞/0 | F=200 |
- The thickness of the sheet can range from 20µm to 2µm depending on the number of tiles.
- The sample moves to theoretically image samples just under 1cm3 in size.
- 488nm
- 561nm
- Hammatsu Flash 4 v3