Image Analysis

The University Imaging Centers provides image data analysis services to aid clients in generating quantifiable data from digital images, be it histological or fluorescence microscopy, 2D or 3D fixed cell or time-lapse, or large volume tissue data. 

Images are processed and analyzed with either open source software, such as FIJI, QuPath, or CellProfiler, or commercial software available on UIC workstations, including Living Image, Vevo, AutoQuant, PicoQuant, Nikon Elements, and Imaris. Typically, images are processed to remove background noise, then segmented and analyzed to generate colocalization data, time-lapse intensity profiles, morphometry, distance relationships, particle analyses, etc. The raw and processed images, resulting data, and image analysis methods are then copied to Google Drive and shared with the client. 

Image analysis methods can be devised by UIC staff and taught to clients so that they may execute the analyses themselves. Instruction in the use of image analysis software is provided to clients as well. Periodic webinars and workshops in various image analysis methods are given to registered clients. UIC staff members are proficient in the aforementioned software and can customize image analysis methods to generate the most accurate data possible.

The UIC has expanded its Image Analysis Services to include Image Analysis Office Hours at all three locations, offering support for researcher image analysis and processing workflows. Image Analysis Office Hours are available to researchers at no additional cost.

Office Hours:

• Mondays: 9 AM – 12 PM, room 1-153, Jackson Hall
• Mondays: 2 PM – 4 PM, room 23, Snyder Hall
• Fridays: 9 AM – 12 PM, room 1-220 CCRB

These office hours are intended to help users:

• Develop effective image processing and analysis strategies
• Get guidance on selecting and using analysis software
• Troubleshoot specific questions related to image analysis workflows

Larger or more complex projects, such as designing an extensive GA3 processing and analysis pipeline or performing a full end-to-end analysis, will require a separate Image Analysis service appointment.