immunology-shared services

Employee Services

These shared resources are available to other researchers both within and outside the University of Minnesota community.

They provide a stable, cost-effective, and technologically relevant means of supporting the Center for Immunology's mission to coordinate and promote efforts in immunology research, education, and clinical applications. 

Location: Winston and Maxine Wallin Medical Bioscience Building (WMBB)

Contact: Deb Lins, [email protected], 612-626-2476

Lab safety training is required for everyone using the equipment and facilities.

View Lab safety training and guidelines for New and existing employees  

CFI Shared Resources

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CFI Shared Resources

RS 2000 X-Ray Irradiator

Immunology-RS 2000

RS 2000 Xray

The RS 2000 is the non-isotope alternative to radioactive source equipment for research irradiation applications. This system is powerful and flexible enough to assist with biological studies in cancer, biotechnology, and selected institutional work:

  • Human Genome
  • Myeloablation
  • Stem Cell Research
  • Small animal work
  • Feeder cell production

The RS 2000 has user-variable dose rates up to 6 Gy per minute, whereas a gamma irradiator's dose rate is dependent on the isotope used and the remaining curies encapsulated. Rad Source has developed the expertise to produce a dose equivalency to gamma irradiators.
 
The RS 2000 allows for one cycle irradiation of animals in a standard filtered Allentown Cage, which is provided with the equipment and provides 95% horizontal dose uniformity within the rearing cage using our patented reflective holder
 
See radsource.com for more information about this product.

Request training for the X-Ray Irradiator or visit the Xray Irradiator page 

or email Deb Lins

CFI Instrumentation

A variety of common laboratory instrumentation is also shared by the center staff members. A sampling of this equipment encompasses centrifugation, spectrophotometry, real-time PCR, and protein purification.

  • C.T.L. ImmunoSpot Elispot
  • Molecular Devices- Spectra Max 384 Plus Plate Reader
  • Savant SpeedVac Rotovap
  • TECAN Infinite Pro M Plex
  • Thermo Scientific NanoDrop OneC
  • UVP EL3 Gel Documentation System

If you need access to equipment, please contact CFI at [email protected] or Deb Lins at [email protected] for more information.

Flow Cytometry

Please visit the University Flow Cytometry Resource (UFCR) website for all of your flow cytometry needs.

WMBB machines located in Location: 2-289 WMBB 

CFI Imaging

CFI maintains an imaging facility in WMBB. 

Equipment available for use:

  • Leica Stellaris 8 DIVE white light laser confocal microscope with dual multi-photon Mai Tai and Insight lasers
  • Leica Stellaris 8 white light laser inverted confocal microscope
  • Leica DM6000 Thunder epifluorescent microscope
  • Leica CM1800 Cryostat
  • Leica 1000S Vibratome
  • Analysis Workstations with Halo and Imaris software

Usage rate: $45 per hour

To schedule a time, use this link to check the calendar
Email Jason Mitchell or Brian Fife to learn more and request training.

University Imaging Centers

The University Imaging Centers cover over 11,000 square feet of resources in three primary sites on the University of Minnesota-Twin Cities campuses. Snyder Hall on the St. Paul campus, Jackson Hall on the Minneapolis East Bank campus, and the Cancer and Cardiovascular Research Building located in the Biomedical Discovery District on the East Bank campus. The network of facilities offers instrumentation and support for light and electron microscopy, multiplex ion beam imaging, tissue clearing, in-vivo imaging from subcellular to whole animals. UIC Staff are available to assist in experimental design, probe and labeling selection, sample handling, tissue clearing, as well as image analysis & visualization, slide scanning, large format poster and 3D printing.

In addition to training users to independently operate our instrumentation the University Imaging Centers also offers several end-to-end research and full sample preparation services.

For a complete list of services offered, visit the UMN Imaging Center website

Services offered:

  • CONFOCAL MICROSCOPES
  • LIGHT SHEET MICROSCOPES
  • LIGHT MICROSCOPES
  • SLIDE SCANNING
  • MIBI
  • ELECTRON MICROSCOPES
  • SMALL ANIMAL IMAGING
  • MOLECULAR BIOLOGY IMAGERS
  • REMOTE SUPPORT & ONLINE LEARNING RESOURCES

 

​​​​​​​Bio-Medical Library
 Research Animal Resources Electronic Instrument Services Wiley's Current Protocols

RFSS Contacts

Who to contact 

Fill out a non-urgent work request form for all building and FM requests

For Urgent work requests(floods, alarms, card reader doors, etc.), protocol on the contact page:

During business hours

  1. Information Desk open 8 a.m. to 4 p.m. Monday to Friday
    phone:612-626-5258
    Email: [email protected]
  2. James Gladney
    LRB/MTRF & WMBB Building Coordinator
    Phone: (612) 301-5884
    Email: [email protected]

After business hours, weekends, and urgent needs
RFSS After Hours Emergency
On-Call Phone
(763) 760-4794

Radiation and Chemical Spills:

  • During business hours, call 612-626-6002.
  • After business hours, call 911 from a University phone.

For more information about research facility support at the University of Minnesota's Academic Health Center, visit Research Facility Support Services (RFSS)