immunology-shared services

Employee Services

These shared resources are available to other researchers both within and outside the University of Minnesota community.

They provide a stable, cost-effective, and technologically relevant means of supporting the Center for Immunology's mission to coordinate and promote efforts in immunology research, education, and clinical applications. 

Location: Winston and Maxine Wallin Medical Bioscience Building (MBB)

Contact: Deb Lins, benso010@umn.edu, 612-626-2476

Lab safety training is required for everyone using the equipment and facilities.

Lab safety training and guidelines for New and existing employees

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RS 2000 X-Ray Irradiator

Immunology-RS 2000

The RS 2000 is the non-isotope alternative to radioactive source equipment for research irradiation applications. This system is powerful and flexible enough to assist with biological studies in cancer, bio-technology and selected institutional work including:

  • Human Genome
  • Myeloablation
  • Stem Cell Research
  • Small animal work
  • Feeder cell production

The RS 2000 has user-variable dose rates up to 6 Gy per minute whereas a gamma irradiator's dose rate is dependent on the isotope used and the remaining curies encapsulated. Rad Source has developed the expertise to produce a dose equivalency to gamma irradiators.
 
The RS 2000 allows for one cycle irradiation of animals in a standard filtered Allentown Cage, which is provided with the equipment and provides 95% horizontal dose uniformity within the rearing cage using our patented reflective holder
 
See radsource.com for more information about this product.

Request training for the X-Ray Irradiator 

or contact Deb Lins

CFI Instrumentation

A variety of common laboratory instrumentation is also shared by the center staff members. A sampling of this equipment encompasses centrifugation, spectrophotometry, real-time PCR, and protein purification.

  • C.T.L. ImmunoSpot Elispot
  • Molecular Devices- Spectra Max 384 Plus Plate Reader
  • Savant SpeedVac Rotovap
  • TECAN Infinite Pro M Plex
  • Thermo Scientific NanoDrop OneC
  • UVP EL3 Gel Documentation System

If you need access to equipment please contact CFI at cfi@umn.edu or Deb Lins at benso010@umn.edu for more information.

Flow Cytometry

Please visit the University Flow Cytometry Resource (UFCR) website for all of your flow cytometry needs.

WMBB machines:

BD LSR II (H10100002)

 

BD LSR II

BD LSR II (H10100002)|Online Calendar|Configuration|Fluorescent Guide

  • 4 lasers: 50mW Violet (405nm), 20mW Blue (488nm), 40mW Yellow-Green (561nm) and 40mW Red (640nm) spatially-separated laser sources
  • 14 fluorescent parameters (6V-2B-3YG-3R)

BD LSRFortessa (H17700017)

 
BD LSRFortessa

BD LSRFortessa (H17700017)|Online Calendar|Configuration| Fluorescent Guide

  • 5 lasers: 20mW UV (355nm), 100mW Violet (405nm), 50mW Blue (488nm), 40mW Yellow-Green (561nm), 40mW Red (640 nm) spatially-separated laser sources
  • 18 fluorescent parameters (4UV-6V-2B-3YG-3R)

Location: 2-289 Wallin Medical Biosciences Building (WMBB)

CFI Imaging

CFI maintains an imaging facility in WMBB. 

Equipment available for use:

  • Leica TCS Multiphoton Microscope
  • Leica DM5500B Epifluorescent Wide-field microscope
  • Olympus FV1000 Confocal microscope
  • Leica CM1800 Cryostat
  • Vibratome

To schedule use, please check the calendar.
To learn more information and receive training, talk to Jason Mitchell or Brian Fife.

University Imaging Centers

The University Imaging Centers cover over 11,000 square feet of resources in three primary sites on the University of Minnesota-Twin Cities campuses. Snyder Hall on the St. Paul campus, Jackson Hall on the Minneapolis East Bank campus, and the Cancer and Cardiovascular Research Building located in the Biomedical Discovery District on the East Bank campus. The network of facilities offers instrumentation and support for light and electron microscopy, multiplex ion beam imaging, tissue clearing, in-vivo imaging from subcellular to whole animals. UIC Staff are available to assist in experimental design, probe and labeling selection, sample handling, tissue clearing, as well as image analysis & visualization, slide scanning, large format poster and 3D printing.

In addition to training users to independently operate our instrumentation the University Imaging Centers also offers several end-to-end research and full sample preparation services.

 

Services offered:

​​​​​​​Bio-Medical Library
 Research Animal Resources Electronic Instrument Services Wiley's Current Protocols

RFSS Contacts

Who to contact 

Fill out a non-urgent work request form for all building and FM requests

For Urgent work requests(floods, alarms, card reader doors, etc.):

During business hours

  1. Information Desk: 612-626-5258 or RBMSBDDW@umn.edu (8 a.m. - 4 p.m. Monday - Friday)
  2. James Gladney: 612-301-5884 (office)
  3. Cheryl Miller: 612-625-5905 (office)

After business hours, weekends, and urgent needs

  1. Building Coordinator on Call: 763-760-4794 (cell)
  2. Cheryl Miller: 651-769-4664 (cell)

Radiation and Chemical Spills:

  • During business hours call 612-626-6002.
  • After business hours call 911 from a University phone.