Metabolomics Core

Targeted Metabolomics Assay Modules

Activity Assay
#1 Total ketone body in serum
#2 Total ketone body in tissue
#3 Enantiomers βOHB/2-OHB/3-HIB
#4 Total ketone body and enantiomers of βOHB in tissue
#5 13^C- Total ketone body in serum
#6 CoA (4) in tissue
#7 Nucleotides (5) in tissue
#8 CoA (4) and nucleotides (5) in tissue
#9 Total ketone body, CoA and nucleotides in tissue
#10 Total ketone body, enantiomers BOHB, CoA (4) and nucleotides (5) in tissue
#11 Shotgun lipidomics of 4-hydroxynonenal species (5)
#12 Acetate, butyrate and propionate

Total ketone body= Concentration of AcAc and DL-βOHB
2-OHB= 2-hydroxybutyrate
3-HIB= 3-hydroxyisobutyrate
CoA (4)= acetyl-CoA, malonyl-CoA, priopionyl-CoA, succinyl-CoA
Nucleotides (5)= AMP, ADP, ATP, NAD+, NADH
4-hydroxynonenal species: HHE, HNDE, HNE, HDTE, HDDE 

For more information please contact: ppuchals@umn.edu