Anthony D. Baughn, PhD

Associate Professor, Department of Microbiology and Immunology

Anthony D. Baughn

Contact Info

abaughn@umn.edu

Office Phone 612-626-0460

Fax 612-626-0623

Lab Phone 612-625-9959

Office Address:
4-115 MRF
689 23rd Ave SE
Minneapolis, MN 55455

Mailing Address:
Department of Microbiology and Immunology
4-115 Microbiology Research Facility (MRF)
CDC: 2821A
689 23rd Ave SE
Minneapolis, MN 55455-1507

PhD, Tufts University, 2004

Research

Research Summary/Interests

Metabolic requirements for bacterial persistence

The ability of Mycobacterium tuberculosis (Mtb) to persist in the presence of anti-mycobacterial drugs and immune effector functions is the greatest obstacle in our attempt to eradicate tuberculosis (TB). My laboratory is investigating the hypothesis that persistence is enabled by entrance into and maintenance of a physiological state that is metabolically distinct from that of actively replicating bacilli. The aim of this work is to define this state (or series of states) and characterize pathways that are essential for its establishment and maintenance. My laboratory uses a variety of classical and novel approaches in mycobacterial genetics, biochemistry, physiology, and metabolomics to characterize i) novel aspects of intermediary metabolism in Mtb, ii) metabolic requirements for phenotypic drug tolerance, and iii) drug targets and drug-like compounds effective against persistent bacilli. While these studies address basic science questions about unique aspects of Mtb, they will also provide important contributions to the collaborative and global effort to eradicate TB.

Publications

Structure-Aware Mycobacterium tuberculosis Functional Annotation Uncloaks Resistance, Metabolic, and Virulence Genes.

Modlin SJ, Elghraoui A, Gunasekaran D, Zlotnicki AM, Dillon NA, Dhillon N, Kuo N, Robinhold C, Chan CK, Baughn AD, Valafar F. mSystems. 2021 Nov 2;6(6):e0067321. doi: 10.1128/mSystems.00673-21. Online ahead of print. PMID: 34726489

Pathogen-specific antimicrobials engineered de novo through membrane-protein biomimicry.

Simonson AW, Mongia AS, Aronson MR, Alumasa JN, Chan DC, Lawanprasert A, Howe MD, Bolotsky A, Mal TK, George C, Ebrahimi A, Baughn AD, Proctor EA, Keiler KC, Medina SH. Nat Biomed Eng. 2021 May;5(5):467-480. doi: 10.1038/s41551-020-00665-x. Epub 2021 Jan 4. PMID: 33390588

Genome-wide identification of essential genes in Mycobacterium intracellulare by transposon sequencing - Implication for metabolic remodeling.

Tateishi Y, Minato Y, Baughn AD, Ohnishi H, Nishiyama A, Ozeki Y, Matsumoto S. Sci Rep. 2020 Mar 25;10(1):5449. doi: 10.1038/s41598-020-62287-2. PMID: 32214196

The Bewildering Antitubercular Action of Pyrazinamide.

Lamont EA, Dillon NA, Baughn AD. Microbiol Mol Biol Rev. 2020 Mar 4;84(2):e00070-19. doi: 10.1128/MMBR.00070-19. Print 2020 May 20. PMID: 32132245

Impact of the host environment on the antitubercular action of pyrazinamide.

Lamont EA, Baughn AD. EBioMedicine. 2019 Nov;49:374-380. doi: 10.1016/j.ebiom.2019.10.014. Epub 2019 Oct 25. PMID: 31669220

Revitalizing antifolates through understanding mechanisms that govern susceptibility and resistance.

Kordus SL, Baughn AD. MedChemComm. 2019 May 8;10(6):880-895. doi: 10.1039/c9md00078j. eCollection 2019 Jun 1. PMID: 31303985

Genomewide Assessment of Mycobacterium tuberculosis Conditionally Essential Metabolic Pathways.

Minato Y, Gohl DM, Thiede JM, Chacón JM, Harcombe WR, Maruyama F, Baughn AD. mSystems. 2019 Jun 25;4(4):e00070-19. doi: 10.1128/mSystems.00070-19. PMID: 31239393

Methionine Antagonizes para-Aminosalicylic Acid Activity via Affecting Folate Precursor Biosynthesis in Mycobacterium tuberculosis.

Howe MD, Kordus SL, Cole MS, Bauman AA, Aldrich CC, Baughn AD, Minato Y. Front Cell Infect Microbiol. 2018 Nov 12;8:399. doi: 10.3389/fcimb.2018.00399. eCollection 2018. PMID: 30483484

Mutual potentiation drives synergy between trimethoprim and sulfamethoxazole.

Minato Y, Dawadi S, Kordus SL, Sivanandam A, Aldrich CC, Baughn AD. Nat Commun. 2018 Mar 8;9(1):1003. doi: 10.1038/s41467-018-03447-x. PMID: 29520101

Ribosome Rescue Inhibitors Kill Actively Growing and Nonreplicating Persister Mycobacterium tuberculosis Cells.

Alumasa JN, Manzanillo PS, Peterson ND, Lundrigan T, Baughn AD, Cox JS, Keiler KC. ACS Infect Dis. 2017 Sep 8;3(9):634-644. doi: 10.1021/acsinfecdis.7b00028. Epub 2017 Aug 7. PMID: 28762275