The main goal of my research is to explore the molecular pathways/factors that control tumor development and progression in the central nervous system. My ultimate objective is to translate our molecular mechanistic knowledge into clinical applications by developing novel therapeutic drugs and early biomarker screening tools for brain tumors. My strategy involves comprehensive analyses of patient-derived specimens such as tumor tissue/cells and sera through use of cutting-edge technologies such as next-generation sequencing, RNA sequencing, miRNA and drug screening, gene arrays, and proteomics in order to identify tumor-specific molecular targets that can be used for novel drug development or employed as early tumor biomarker.
My research topics are as follows:
Clinical Biomarker Development Studies for brain tumors:
Glioblastomas: we are working on several clinical biomarker development studies for glioblastomas using serum, tissues and extracellular vesicles. We are employing comprehensive analyses of tumor tissues/cells through the high-throughput screening methodologies such as proteomics, gene arrays, next generation sequencing (NGS), tumor-associated autoantibody (TAA) array, and Proseek Multiplex Cancer Panel, directing for discovery of novel drug targets and biomarkers.
Meningiomas: we are performing similar screening studies (see above) for meningioma patients as well and develop a panel consisting of 5 circulating protein biomarkers which can be useful in the clinical practice to monitor transition of meningiomas from benign to malignant forms.
Medulloblastomas: we recently discovered an oncogene in the extracellular vesicles of medulloblastoma patients that can be used as a worldwide screening tool in newborns for early detection of medulloblastomas. Additionally, we also developed a nano-string based panel biomarkers that can be useful to genetically diagnose sub-types of medulloblastomas in the patient serums.
Genetically engineering extracellular vesicles (EVs) as a novel small-molecule delivery tool for brain tumors: we have recently developed genetically engineered EVs by expressing high levels of the mRNA and protein (Mizrak et al., 2013; Erkan et al.: 2016 and 2017). We are now using these molecules as a miRNA/RNA/protein and small molecule delivery tool to treat brain tumors
High-throughput drug discoveries for brain tumors: another topic of my research is to perform High-throughput Drug Discovery Studies (HTDDS) for brain tumors using the several libraries consisting of FDA approved drugs and bioactive compounds.
