Scott M OGrady

Bio

Areas of Interest

Cellular Physiology

Research Summary

Investigations in my laboratory focus on understanding mechanisms and regulation of electrolyte transport across epithelial tissues and on innate immune function of the airway epithelium in relation to cystic fibrosis, asthma and allergic airway disease. Cultured human airway epithelial cells are currently used to study regulation of ion transport and cell migration by a variety of signaling molecules. A combination of electrophysiological approaches and molecular techniques provide a means of identifying transport mechanisms and signaling pathways that are important in controlling salt and fluid movement across the airway epithelium. Studies of innate immune function are being conducted in collaboration with Dr. Hirohito Kita from the Mayo Clinic. These studies address the role of the airway epithelium in allergic inflammation induced by environmental allergens and fungal infection of the lung.

Publications

Refereed Articles

Srisomboon, Y., K. Iijima, M. Colwell, P.J. Maniak, Marissa Macchietto, C. Faulk, H. Kita, S.M. O’Grady, Allergen-induced DNA release by the airway epithelium amplifies type 2 immunity, J. Allergy Clin. Immunol. https://doi.org/10.1016/j.jaci.2022.09.034 (Oct 25, 2022) (IF = 14.29)

Srisomboon, Y., Ohkura, N., Iijima, K., Kobayashi, T., Maniak, P.J., Kita, H., O’Grady, S.M., Airway exposure to polyethyleneimine nanoparticles induces type 2 immunity by a mechanism involving oxidative stress and ATP release. International Journal of Molecular Sciences, 22 (16), 9071-9089, 2021.

Srisomboon Y, Squillace DL, Maniak PJ, Kita H, O'Grady SM. Fungal allergen-induced IL-33 secretion involves cholesterol-dependent, VDAC-1-mediated ATP release from the airway epithelium. J Physiol., 598 (10):1829-1845. 2020.

Editors’ Choice Award: The Journal of Physiology. This paper was highlighted in the Perspectives article by: Ahmad S and A. Ahmad. Treating fungus-induced allergic asthma: Do VDACs have the answer! J Physiol. 598 (10), 1799–1800, 2020.

Journal Cover: The editors of The Journal of Physiology chose Fig 5C from this paper as the cover image for volume 598 (10), 2020. 

Graeff R, Guedes A, Quintana R, Wendt-Hornickle E, Baldo C, Walseth T, O'Grady S, Kannan M. Novel Pathway of Adenosine Generation in the Lungs from NAD(+): Relevance to Allergic Airway Disease. Molecules, 25 (21), 1-18, 2020.

Refereed Articles on PubMed

Book Chapter

O’Grady, SM, Ion Transport: Potassium Channels, Encyclopedia of Respiratory Medicine, 2nd Edition, Volume 1, pp 669-677, 2021.

O’Grady, S.M. (Invited review) Ion Transport, Potassium Channels. Encyclopedia of Respiratory Medicine In Press. 2020.

O’Grady, S.M. 2017. CFTR involvement in cell migration and epithelial restitution. In: Progress in Understanding Cystic Fibrosis. D.D. Sriramulu, Ed., Rijeka: InTech; 2017, pp 1-22.

Bankers-Fulbright, J. and S.M. O'Grady. 2012. Mechanisms of eosinophil degranulation: pH Regulation and release of major basic protein. In: Eosinophils: Structure, Biological Properties and Role in Disease. NOVA Science Publishers, Hauppauge, NY.

Brown, D.R. and S.M. O'Grady. 2008. The Ussing chamber and measurement of drug actions on mucosal ion transport. In: Current Protocols in Pharmacology. John Wiley, NY, 41:7.12.1-7.12.17, 2008.

Abstracts

Collins, M., M. Imbrogno, E. Kramer, J. Brewington, N. Zhang, P. Sharon, E. Kopras, J Meeker, H. Morgan, A. Ostmann, S. O'Grady, B. Trapnell, J. Clancy, K. Hudock, Neutrophil extracellular traps disrupt bronchial epithelial barrier function: Alpha-1 antitrypsin to the rescue. J. Cystic Fibrosis, 21: S223, 2022

Iijima, K., T. Kobayashi, K. Ohara, K. Matsumoto, R. Tei, S. O'Grady, H. Kita, Allergen-induced extracellular release of self-DNA promotes innate type 2 immune responses in the lung, J. Allergy Clin Immunol., 149: AB75, 2022

Almutlaq, R., A. Dayton, J. Ross, Y. Srisomboon, S. O'Grady, L.C. Evans, Exposure to excess albumin causes inflammatory activation of the proximal tubule, Hypertension, 79: AP082, 2022.

Moore, P., Wiggen, T., Kent, L., Arif, S., Lucas, S., O’Grady, S.M. Hunter, R., Anaerobic microbiota facilitate Pseudomonas aeruginosa access to the airway epithelium in a novel co-culture model of colonization. Journal of Cystic Fibrosis, 20, S195, 2021.

Ohara, K., Iijima, K., O'Grady, S.M., Kita, H., Detergent induces active release of IL-33 from airway epithelial cells and promotes innate type 2 responses in the lungs. Journal of Allergy and Clinical Immunology 147 (2), AB38, 2021.

Srisomboon, Y,  Maniak PJ,  Kita H, O'Grady SM. K2P channel inhibition enhances allergen‐induced ATP release by a mechanism involving PKC activation in human bronchial epithelial cells. FASEB J. 34(S1): doi.org/10.1096/fasebj.2020.34.s1.05204.

Srisomboon Y, Maniak PJ, Squillace D, Kita H, O’Grady SM. Simvastatin Inhibits Allergen-induced IL-33 Secretion in Human Bronchial Epithelial Cells by Blocking ATP Release and Ca2+ Uptake. FASEB J 33: 734.5-734.5, 2019.

Presentations

O' Grady, S.M. Invited Seminar: City-wide Pulmonary Research Conference, Minneapolis MN, 06/25/2021. "Vital DNA Release by the Airway Epithelium and its role in Inflammation."

O' Grady, S.M. Invited Speaker (Zoom Presentation): Department of Biology, Virginia Commonwealth University, Richmond VA, 10/07/2020: “VDAC-1 mediated ATP release and type 2 immunity in airways”.

O' Grady, S.M. 2019. Epithelial ETosis and airway host-defense following fungal aeroallergen exposure. CVM Research Seminar, St. Paul, MN. March 6. 

O' Grady, S.M. 2018. Glucocorticoid regulation of airway epithelial ion transport during mucociliary differentiation. Department of Medicine, Minneapolis, MN. November 14.

Invited Reviews

Ingbar, D.H., M. Bhargara, and S.M. O'Grady. 2009. Mechanisms of alveolar epithelial chloride absorption. Am. J. Physiology [Lung Cell and Mol. Physiol.] 297: L813-L815.

Chanson, M., B.A. Kotsias, C. Peracchia, and S.M. O'Grady. 2007. Interaction of gap junction channels with other membrane channels and transporters. Prog. Biophys. Mol. Biol. 94:233-244.

O'Grady, S.M. 2006. Ion transport: Potassium channels. Encyclopedia of Respiratory Medicine 8:477-482.

Palmer, M.L., A.C. Fahrenkrug, and S.M. O'Grady. 2006. RNA interference and ion channel physiology. Cell Biochem. Biophys. 46:1-17.

O'Grady, S.M. and S.Y. Lee. 2005. Molecular diversity and function of voltage-gated (Kv) potassium channels in epithelial cells. Int. J. Biochem. Cell Biol. 37:1578-1594.

Lee, S.Y. and S.M. O'Grady. 2003. Modulation of ion channel function by P2Y receptors. Cell Biochem. Biophys. 39:75-88.

O'Grady, S.M. and S.Y. Lee. 2003. Chloride and potassium channels in alveolar epithelial cells. Am. J. Physiology (Lung Cell and Molecular Physiol.) 284:L689-L700.

O'Grady, S.M., X. Jiang, and D.H. Ingbar. 2000. CL channel activation is necessary for stimulation of amiloride-sensitive NA transport in cultured adult rat alveolar epithelial cells. Am. J. Physiol. [Lung Cell and Molecular Physiology] 278:L239-L244.

Honors and Awards

• Dec 2022: Editor’s Choice Recognition; Journal of Allergy and Clinical Immunology.
• Oct 2022: Star Reviewer Award; American Journal of Physiology [Cell Physiology].
• May 2020: Editor’s Choice Recognition; Journal of Physiology.
• Jul 2017: Editor's Choice Recognition; Journal of Physiology.
• Jun 2017: APSselect Award; American Physiological Society.
• Apr 2017: Paper of the Year Award; American Journal of Physiology [Cell Physiology].
• Aug 2016: APSselect Award; American Physiological Society.
• Apr 2012: Star Reviewer Award; American Journal of Physiology [Cell Physiology].
• Jul 2007: Teaching excellence in Veterinary Physiology Award, College of Veterinary Medicine, University of Minnesota.
• May 1990: Gordon L. Starr Outstanding Faculty Contribution Award, University of Minnesota.

Teaching Summary

ANSC/PHSL 5702; PHSL 4702: Cell Physiology

Editorial Service

Editorial Board and Topic Editor, International Journal of Molecular Sciences 

Editorial Advisory Board, American Journal of Physiology [Cell Physiology].

Editorial Advisory Board, International Archives of Clinical Pharmacology.