James R. Dutton, PhD

Associate Professor, Department of Genetics, Cell Biology and Development

James R. Dutton

Contact Info

dutto015@umn.edu

Office Phone 612-626-2762

Office Address:
Stem Cell Institute
2-220 MTRF
2001 6th St SE
Minneapolis, MN 55455

Mailing Address:
Stem Cell Institute
LRB/MTRF
2873B (Campus Delivery Code)
2001 6th St SE
Minneapolis, MN 55455

PhD, University of Wales (A Genetic and Biochemical Study of Microbial Phthalate Degradation), 1992

BSc, University of Cardiff (Microbiology with Genetics), 1988

Summary

Expertise

Cell reprogramming, Induced Pluripotent cells

Professional Associations

  • iPS Cell Facility Coordinator, Stem Cell Institute, University of Minnesota, Minneapolis, 2008
  • Assistant Professor Stem Cell Institute, University of Minnesota, Minneapolis, USA, 2007
  • Research Coordinator, Prof. Slack group, Centre for Regenerative Medicine, University of Bath, UK, 2004-07
  • Research Associate, Department of Biology and Biochemistry, University of Bath, 1997-04
  • Research Associate, Department of Microbiology, University of Idaho, USA, 1993-97
  • Postdoctoral Researcher, Department of Microbiology, University of Idaho, USA, 1992-93

Research

Research Summary/Interests

My complete research portfolio is now extremely collaborative using the strength of team science to drive success of investigations designed to find new approaches to disease modeling and treatment.  I am the PI of a project using miPSC-derived limb bud progenitors to improve limb and digit regeneration and the Team PI of an NEI-funded RO1 with Prof. Ferrington in the Department of Ophthalmology generating an extensive biobank of iPSC-derived RPE from patients and eye donors with Dry Age-related Macular Degeneration.  These iPSC-RPE provide an excellent experimental model to study mitochondrial dysfunction in AMD and for screening compounds that may maintain or restore mitochondrial function in the RPE of patients with AMD.  I have also developed novel accelerated neural induction protocols to efficiently generate hiPSC-derived neuronal and glial cells for testing regenerative strategies to treat neural injury and disease.  The experience from this work is directly relevant to the current Harrington proposal where we will utilize a similar protocol to generate the cortical neurons from multiple hiPSC lines for drug screening.  The research team I lead also works on a range of other team projects including using iPSCs and blastocyst complementation to generate liver cells, using iPSCs and automation to investigate DMSO-free cryopreservation strategies for stem cells and stem cell-derived products and we are working with the Lions Gift of Sight Eye bank in Minneapolis to derive limbal stem cells from hiPSCs to support potential clinical use of these cells to repair corneal damage.

Publications

  • Walsh P, Truong V, Nayak S, Saldías Montivero M, Low WC, Parr AM, Dutton JR. (2020) Accelerated differentiation of human pluripotent stem cells into neural lineages via an early intermediate ectoderm population.  Stem Cells.  Aug 3. PMID:32745311
  • So S, Lee Y, Choi J, Kang S, Lee JY, Hwang J, Shin J,Dutton JR, Seo EJ, Lee BH, Kim CJ, Mitalipov S, Oh SJ, Kang E. (2020) The Rho-associated kinase inhibitor fasudil can replace Y-27632 for use in human pluripotent stem cell research. PLoS One. May 12;15 (5) PMID:32396545
  • Li R, Hornberger K, Dutton JR, Hubel A. (2020) Cryopreservation of Human iPS Cell Aggregates in a DMSO-Free Solution-An Optimization and Comparative Study. Front Bioeng Biotechnol. Jan 22;8:1. PMID:32039188
  • Crane AT, Aravalli RN, Asakura A, Grande AW, Krishna VD, Carlson DF, Cheeran MC, Danczyk G, Dutton JR, Hackett PB, Hu WS, Li L, Lu WC, Miller ZD, O'Brien TD, Panoskaltsis-Mortari A, Parr AM, Pearce C, Ruiz-Estevez M, Shiao M, Sipe CJ, Toman NG, Voth J, Xie H, Steer CJ, Low WC.  (2019) Interspecies Organogenesis for Human Transplantation. Cell Transplant. 28 (9-10):1091-1105. PMID: 31426664; PMCID: PMC6767879.
  • Joung D, Truong V, Neitzke CC, Guo SZ, Walsh PJ, Monat JR, Meng F, Park SH,Dutton JR, Parr AM, McAlpine MC. (2018) 3D Printed Stem-Cell Derived Neural Progenitors Generate Spinal Cord Scaffolds. Adv Funct Mater. Sep 26; 28 (39) 1801850. PMID:32595422
  • Su L, Kong X, Lim S, Loo S, Tan S, Poh K, Dutton J, Stewart C, Cook S, Su X, Ma J, Zhang J, Ye L. (2018) The prostaglandin H2 analog U-46619 improves the differentiation efficiency of human induced pluripotent stem cells into endothelial cells by activating both p38MAPK and ERK1/2 signaling pathways. Stem Cell Res Ther. 9 (1) 313. PMID: 30442193; PMCID: PMC6238266.
  • Patil N, Truong V, Holmberg MH, Lavoie NS, McCoy MR, Dutton JR, Holmberg EG, Parr AM. (2018) Safety and Efficacy of Rose Bengal Derivatives for Glial Scar Ablation in Chronic Spinal Cord Injury. J Neurotrauma. 35 (15) 1745-1754. PMID: 29373946; PMCID: PMC6033306.
  • Walsh P, Truong V, Hill C, Stoflet ND, Baden J, Low WC, Keirstead SA, Dutton JR, Parr AM. (2017) Defined Culture Conditions Accelerate Small-molecule-assisted Neural Induction for the Production of Neural Progenitors from Human-induced Pluripotent Stem Cells. Cell Transplant. 26 (12) 1890-1902. PMID: 29390875; PMCID: PMC5802631.
  • Hill CM, Banga A, Abrahante JE, Yuan C, Mutch LA, Janecek J, O'Brien T, Graham ML, Dutton JR (2017) Establishing a large animal model for in vivo reprogramming of bile duct cells into insulin secreting cells to treat diabetes. Human Gene Therapy. Clinical Development. 28 (2)  87-95. PMID: 28363269
  • Geng Z, Walsh PJ, Truong V, Hill C, Ebeling M, Kapphahn RJ, Montezuma SR, Yuan C, Roehrich H, Ferrington DA, Dutton JR. (2017) Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PloS One. 12 (3) e0173575  PMCID: PMC5345835