Eric Batchelor, PhD

Assistant Professor, Department of Integrative Biology and Physiology (IBP)

Postdoctoral Fellowship, Harvard Medical School, 2011

PhD, Physics, University of Pennsylvania, 2005

MS, Physics, University of Pennsylvania, 2002

BS, Physics, Villanova University, 1999

Summary

Awards & Recognition

  • Spotlighted Young Investigator, Journal of Molecular Biology Biology Career Advancement Initiative, 2017
  • NCI Director's Innovation Award, 2014
  • American Cancer Society, Pamela and Edward Taft Postdoctoral Fellowship, 2011
  • Harvard University Certificate of Distinction in Teaching, 2006
  • American Heart Association Pre-doctoral Research Fellowship, 2005
  • National Science Foundation Pre-doctoral Research Fellowship, 2002
  • Villanova Presidential Schalr, 1999
  • National Merit Scholar, 1995

Research

Research Summary/Interests

My lab seeks to develop a quantitative, system-level understanding of stress response pathways at multiple scales -- from individual cells to whole organisms.  Much of our research focuses on cell stress responses mediated by the tumor suppressor protein p53.  We have used long-term time-lapse imaging to show that p53 undergoes complex dynamics in response to different forms of DNA damage.  For example, in response to DNA double-strange breaks p53 levels increase and decrease in oscillations of fixed amplitude, duration, and frequency.  In contrast, in response to UV-induced DNA damage p53 levels increased in a single pulse with a duration and amplitude that increases with decreasing damage.  We have identified the specific molecular mechanisms that regulate the distinct p53 dynamical modes.  By using small-molecule inhibitors to alter p53 dynamical modes.  By using small-molecular inhibitors to alter p53 dynamics,we can change the transcriptional program regulated by p53 and thereby control individual cell fates.

Our current work focuses on developing a more integrated, network-level view of the regulation and function of p53 dynamics in single cells. We focus on identifying the molecular details by which p53 dynamics regulate downstream damage response pathways, including those regulating cell cycle arrest, DNA repair, senescence, apoptosis, and metabolism.  We aim to identify how specific p53 pulse characteristics (amplitude, duration, or frequency) encode information that is decoded at the promoters of transcriptional targets.  We are also appying quantitative, single-cell approaches to identify novel modes of regulation between p53 and other important signaling pathways, including the MYC proto-oncogene network and MapK signaling.  By developing a more quantitative understanding of these important signaling pathways, we hope to not only increase our basic understanding of these signaling dynamics in regulating cell fate decisions, but also provide. novel methods for chemotherapeutic manipulation of signaling dynamics to alter cell fate in cancers in which the pathways are deregulated.

Publications

  • Harton MD, Koh WS, Bunker AD, Singh A, and Batchelor E. p53 pulse modulation differentially regulates target gene promoters to regulate cell fate decisions. Mol Syst Biol (2019) Sep; 15(9):e8685. PMID: 31556489.
  • Hanson, RL, Porter JR, and Batchelor E. Protein stability of p53 targets determines their temporal expression patterns in response to p53 pulsing. J Cell Biol 2019 Apr 1; 218(4):1282-1297. PMID:30745421
  • Porter JR, Fisher BE, Baranello L, Liu JC, Kambach DM, Nie Z, Koh WS, Luo J, Stommel JM, Levens D, and Batchelor E.Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response. Mol Cell 2017 Sep 21;67(6):1013-1025. PMID: 28867293.
  • Porter JR, Fisher BE, and Batchelor E. p53 pulses diversify target gene expression in an mRNA half-life-dependent manner and delineate co-regulated target gene subnetworks. Cell Systems. 2016; 2(4):272-82. PMID: 27135539.